Cell population evolution in tissue cultures from embryo barley (Hordeum vulgare L.) after caffeine treatment

PROTOPLASMA ◽  
1981 ◽  
Vol 107 (1-2) ◽  
pp. 13-20 ◽  
Author(s):  
M. L. Ruiz ◽  
A. M. V�zquez
Euphytica ◽  
1996 ◽  
Vol 91 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Ana Isabel González ◽  
Maria Isabel Peláez ◽  
María Luisa Ruiz

Author(s):  
Renata Orłowska ◽  
Joanna Machczyńska ◽  
Sylwia Oleszczuk ◽  
Janusz Zimny ◽  
Piotr Tomasz Bednarek

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 261-266 ◽  
Author(s):  
M. Molnár-Láng ◽  
G. Galiba ◽  
G. Kovács ◽  
J. Sutka

A total of 41 regenerant plants were raised from two consecutive in vitro propagation cycles from a barley (Hordeum vulgare L. cv. Martonvásári 50) × wheat (Triticum aestivum L. cv. Chinese Spring) hybrid. The second-cycle regenerants exhibited great variability for most morphological traits. The female fertility of certain regenerant hybrids considerably exceeded that of the initial hybrid, giving substantially higher seed set when pollinated with wheat. This character was transferred to the BC1 and BC2 progeny produced from the regenerants. The cytological analysis of the second-cycle regenerants indicated that these had a higher degree of meiotic instability than the initial hybrid. The proportion of cells with 28 chromosomes (21 wheat + 7 barley) dropped to one-half of that in the initial hybrid, with a rise in the number of hypoploid and hyperploid cells. The number of chiasmata per cell increased from 1.7 in the initial hybrid to 4.7 in the regenerants, and there was also an increase in the number of misdivisions.Key words: intergeneric hybrid, tissue culture, fertility, meiotic behaviour, somaclonal variation.


Author(s):  
R.H.M. Cross ◽  
C.E.J. Botha ◽  
A.K. Cowan ◽  
B.J. Hartley

Senescence is an ordered degenerative process leading to death of individual cells, organs and organisms. The detection of a conditional lethal mutant (achloroplastic) of Hordeum vulgare has enabled us to investigate ultrastructural changes occurring in leaf tissue during foliar senescence.Examination of the tonoplast structure in six and 14 day-old mutant tissue revealed a progressive degeneration and disappearance of the membrane, apparently starting by day six in the vicinity of the mitochondria associated with the degenerating proplastid (Fig. 1.) where neither of the plastid membrane leaflets is evident (arrows, Fig. 1.). At this stage there was evidence that the mitochondrial membranes were undergoing retrogressive changes, coupled with disorganization of cristae (Fig. 2.). Proplastids (P) lack definitive prolamellar bodies. The cytoplasmic matrix is largely agranular, with few endoplasmic reticulum (ER) cisternae or polyribosomal aggregates. Interestingly, large numbers of actively-budding dictysomes, associated with pinocytotic vesicles, were observed in close proximity to the plasmalemma of mesophyll cells (Fig. 3.). By day 14 however, mesophyll cells showed almost complete breakdown of subcellular organelle structure (Fig. 4.), and further evidence for the breakdown of the tonoplast. The final stage of senescence is characterized by the solubilization of the cell wall due to expression and activity of polygalacturonase and/or cellulose. The presence of dictyosomes with associated pinocytotic vesicles formed from the mature face, in close proximity to both the plasmalemma and the cell wall, would appear to support the model proposed by Christopherson for the secretion of cellulase. This pathway of synthesis is typical for secretory glycoproteins.


Author(s):  
А.В. ЖЕЛЕЗНОВ ◽  
◽  
Н.Б. ЖЕЛЕЗНОВА ◽  
Т.В. КУКОЕВА ◽  
Н.В. БУРМАКИНА ◽  
...  

Author(s):  
А.В. ДИКАРЕВ ◽  
◽  
В.Г. ДИКАРЕВ ◽  
Н.С. ДИКАРЕВА ◽  
С.А. ГЕРАСЬКИН ◽  
...  

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