scholarly journals Prognostic Value of Multiple Reverse Transcription-PCR Tyrosinase Testing for Circulating Neoplastic Cells in Malignant Melanoma

2003 ◽  
Vol 49 (9) ◽  
pp. 1450-1457 ◽  
Author(s):  
Jolanta Szenajch ◽  
Bogdan Jasiński ◽  
Agnieszka Synowiec ◽  
Jadwiga Kulik ◽  
Małgorzata Chomicka ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Malignant Melanoma ◽  
Reverse Transcription ◽  
Multiple Testing ◽  
Prognostic Value ◽  
Prognostic Significance ◽  
Detection Threshold ◽  
Logistic Function ◽  
Reverse Transcription Pcr ◽  
Increased Risk

Abstract Background: The reverse transcription-PCR tyrosinase assay (TYR test) cannot reliably detect malignant melanoma (MM) cells in blood as the cells often circulate at low concentrations. We evaluated the prognostic value of multiple TYR testing, the prognostic significance of individual positive TYR test results (TYR+) in asymptomatic melanoma patients, and whether statistical analysis could help in the interpretation of results of a test that measures phenomena that typically occur below its detection threshold. Methods: MM patients in stages I-IV (n = 150) underwent multiple testing with the TYR test during the course of their disease. TYR testing was performed as described by Smith et al. (Lancet 1991;38:1227–9). Statistical analyses were performed with the logistic function and t-test procedures. Results: The relationship between MM stage and the frequency of TYR+ was statistically significant (P = 0.011). Higher frequency of TYR+ in clinically asymptomatic patients after complete resection of the primary tumor was associated with an increased risk of recurrence of MM (prognostic sensitivity, 62%; specificity, 78%). Conclusions: A single positive TYR test provides a warning for disease relapse, suggesting that multiple TYR testing might provide more reliable predictions of disease progression. Multiple testing and statistical analysis using a logistic function might allow for the interpretation of apparently inconsistent results of tests for very rare cells.

scholarly journals Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus, Beet Soilborne Virus, and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet

2003 ◽  
Vol 69 (4) ◽  
pp. 2356-2360 ◽  
Author(s):  
Alexandre Meunier ◽  
Jean-François Schmit ◽  
Arnaud Stas ◽  
Nazli Kutluk ◽  
Claude Bragard
Keyword(s):  
Sugar Beet ◽  
Reverse Transcription ◽  
Detection Threshold ◽  
Simultaneous Detection ◽  
Yellow Vein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Polymyxa Betae ◽  
Reverse Transcription Pcr ◽  
Soilborne Viruses ◽  
Beet Virus Q

ABSTRACT Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and BVQ, together with their vector, P. betae. The detection threshold of the test was up to 128 times greater than that of an enzyme-linked immunosorbent assay. Systematic association of BNYVV with one or two different pomoviruses was observed. BVQ was detected in samples from Belgium, Bulgaria, France, Germany, Hungary, Italy, Sweden, and The Netherlands but not in samples from Turkey.

PROGNOSTIC VALUE OF SERUM URIC ACID IN PATIENTS WITH ACUTE HEART FAILURE:A META-ANALYSIS

2021 ◽  
pp. 44-45
Author(s):  
Mohit Desai ◽  
Brajendra kumar
Keyword(s):  
Uric Acid ◽  
Observational Studies ◽  
Prognostic Value ◽  
Meta Analysis ◽  
Prognostic Significance ◽  
Adverse Outcomes ◽  
Improve Risk Stratification ◽  
Increased Risk ◽  
All Cause Mortality ◽  
Comprehensive Search

Our meta-analysis aimed to determine the prognostic significance of SUA level in patients with AHF.We made a comprehensive search in databases from inception to April 6, 2018. All available observational studies that evaluated the prognostic value of SUA level in patients with AHF were eligible. Outcome of interests were all-cause mortality and the combined endpoint of death or readmission. Prognostic values of SUA level were summarized as higher vs lower SUA category or per 1 mg/ml SUA rise.Eleven studies involving 12,854 AHF patients were identified and analyzed. AHF patients with the highest SUA level had an increased risk of all-cause mortality (risk ratio [RR] 1.43; 95% confidence intervals [CI] 1.31–1.56) and combined endpoint of death or readmission (RR 1.68; 95% CI 1.33–2.13) after adjusting potential variables. In addition, per 1 mg/ml SUA rise significantly increased by 11% and 12% higher risk all-cause mortality and combined endpoint of death or readmission, respectively..This meta-analysis indicates that elevated SUA level independently predicts all-cause mortality and the combined endpoint of death or readmission in AHF patients. Measurement of SUA level may improve risk stratification of adverse outcomes in these patients

scholarly journals Quantification of Melanoma Cell-specific MART-1 mRNA in Peripheral Blood by a Calibrated Competitive Reverse Transcription-PCR

2000 ◽  
Vol 46 (12) ◽  
pp. 1923-1928 ◽  
Author(s):  
Boe Sandahl Sørensen ◽  
Henrik Schmidt ◽  
Hans von der Maase ◽  
Per Thor Straten ◽  
Ebba Nexø
Keyword(s):  
Malignant Melanoma ◽  
Melanoma Cell ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Pcr Amplification ◽  
Melanoma Cells ◽  
Rt Pcr ◽  
Blood Samples ◽  
Reverse Transcription Pcr

Abstract Background: Reverse transcription-PCR (RT-PCR) amplification of melanoma cell-specific mRNA can detect melanoma cells in the peripheral blood of patients with malignant melanoma. We present a method to quantify mRNA coding for the melanoma-specific melanoma antigen recognized by T cells #1 (MART-1) in RNA isolated from peripheral blood. Methods: To establish a calibration curve, we measured the concentration of MART-1 mRNA in SK-MEL-28 melanoma cells grown in vitro by competitive RT-PCR. Serial dilutions of these cells were used as calibrators in the assay. The assay was conducted by adding a fixed amount of a RNA internal standard to RNA isolated from either peripheral blood or the calibrators before RT-PCR amplification with MART-1 primers in a nested PCR design. The amount of MART-1 mRNA in blood samples was calculated from the calibration curve. Results: Addition of melanoma cells grown in vitro to blood from healthy donors demonstrated that the method can detect a single SK-MEL-28 melanoma cell in 1 mL of blood (1.5 × 10−21 mol MART-1 mRNA/mL). MART-1 mRNA was observed in 4 of 12 blood samples from patients with malignant melanoma, at concentrations of 3–18 × 10−21 mol MART-1 mRNA/mL of blood. No MART-1 mRNA was detected in blood samples from 25 controls without malignant melanoma. Intra- and interassay CVs were 15% (n = 12; mean = 44 × 10−21 mol MART-1 mRNA/mL) and 33% (15 samples analyzed in two different analytical runs; mean = 30 × 10−21 mol MART-1 mRNA/mL), respectively. Conclusions: Our method is the first competitive RT-PCR assay for quantification of melanoma cells in blood samples that compensates for the variation of both the reverse transcription and PCR reactions. The method allows the inclusion of control samples for continuous quality assessment.

scholarly journals Molecular Beacon Reverse Transcription-PCR of Human Chorionic Gonadotropin-β-3, -5, and -8 mRNAs Has Prognostic Value in Breast Cancer

2003 ◽  
Vol 49 (7) ◽  
pp. 1074-1080 ◽  
Author(s):  
Paul N Span ◽  
Peggy Manders ◽  
Joop J T M Heuvel ◽  
Chris M G Thomas ◽  
Remko R Bosch ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Chorionic Gonadotropin ◽  
Reverse Transcription ◽  
Human Chorionic Gonadotropin ◽  
Prognostic Value ◽  
Molecular Beacon ◽  
High Expression ◽  
Node Negative ◽  
Reverse Transcription Pcr ◽  
Modified Molecular Beacon

Abstract Background: The β-subunit of human chorionic gonadotropin (hCG) is encoded by four genes, of which expression of the hCGβ-3, -5, and -8 genes could have prognostic value in breast cancer. Methods: Applying a new, modified Molecular Beacon reverse transcription-PCR assay, we investigated the prognostic value of the hCGβ-3, -5, and -8 gene transcripts in 129 sporadic unilateral breast cancer samples from patients with a median follow-up of 62.3 months. Results: Expression of hCGβ-3, -5, -8 was significantly (P = 0.020) associated with relapse-free survival (RFS). In multivariate survival analysis, hCGβ-3, -5, and -8 maintained prognostic value for RFS, with high expression predicting shorter RFS (P = 0.015; hazard ratio, 2.25; 95% confidence interval, 1.17–4.34). Only 1 of 24 (4%) node-negative patients with low hCGβ-3, -5, -8 expression relapsed, in contrast to 7 of 26 (27%) patients with high expression (P = 0.046). Conclusions: Expression of hCGβ-3, -5, -8, which differ by only one nucleotide from other hCGβ genes, can be assessed by our modified Molecular Beacon assay in breast cancer tissues. Expression of hCGβ-3, -5, -8 has independent, prognostic value for RFS in breast cancer and may help identify node-negative patients with poor prognosis.

scholarly journals CD86+/CD206+ tumor-associated macrophages predict prognosis of patients with intrahepatic cholangiocarcinoma

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8458 ◽  
Author(s):  
Dalong Sun ◽  
Tiancheng Luo ◽  
Pingping Dong ◽  
Ningping Zhang ◽  
Jing Chen ◽  
...  
Keyword(s):  
Intrahepatic Cholangiocarcinoma ◽  
Prognostic Value ◽  
Prognostic Significance ◽  
Tumor Development ◽  
Prognostic Index ◽  
Tissue Microarrays ◽  
Prognostic Indicator ◽  
Rank Test ◽  
Tumor Associated Macrophages ◽  
Increased Risk

Background As the main cellular ingredients of tumor microenvironment, tumor-associated macrophages (TAMs) play a vital role in tumor development and progression. Recent studies have suggested that TAMs are sensitive and specific prognostic factors in numerous cancers. The primary purpose of this study is to determine the prognostic significance of TAMs in intrahepatic cholangiocarcinoma (ICC). Methods Immunohistochemical staining of CD68, CD86 and CD206 were performed in tissue microarrays containing 322 patients, who underwent surgical resection and were pathologically diagnosed with ICC. The prognostic value of CD68, CD86 and CD206 were evaluated by Kaplan–Meier analysis (log-rank test) and nomogram models. Results We demonstrated that the CD86+/CD206+ TAMs model was an independent prognostic index for ICC patients. Patients with low CD86+ TAMs and high CD206+ TAMs infiltration had a markedly worse prognosis and increased risk of post-operative recurrence when compared to high CD86+ TAMs and low CD206+ TAMs intratumoral infiltration. Furthermore, subgroup analysis indicated that the CD86+/CD206+ TAMs model predicted prognosis of ICC patients more powerfully than single macrophage immunomarker. Interestingly, the CD86+/CD206+ TAMs model could further distinguish prognosis of CA-199 negative ICC patients, who were generally presumed to have a more favorable outcome. In order to further perfect the prognostic value of the CD86+/CD206+ TAMs model, we constructed and validated a postoperative nomogram to predict overall survival and recurrence-free survival time in ICC patients. Conclusions These findings indicate that the CD86+/CD206+ TAMs model possess potential value as a novel prognostic indicator for ICC patients.

scholarly journals Potential Application of ELAVL4 Real-Time Quantitative Reverse Transcription-PCR for Detection of Disseminated Neuroblastoma Cells

2006 ◽  
Vol 52 (3) ◽  
pp. 438-445 ◽  
Author(s):  
Katrien Swerts ◽  
Barbara De Moerloose ◽  
Catharina Dhooge ◽  
Jo Vandesompele ◽  
Claire Hoyoux ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Real Time ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Mononuclear Cells ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cells ◽  
Reverse Transcription Pcr ◽  
Reliable Detection

Abstract Background: Reliable detection of neuroblastoma cells in bone marrow (BM) is critical because BM involvement influences staging, risk assessment, and evaluation of therapeutic response in neuroblastoma patients. Standard cytomorphologic examination of BM aspirates is sensitive enough to detect single tumor cells. Consequently, more sensitive and specific detection methods are indispensable. Methods: We used real-time quantitative reverse transcription-PCR (QPCR) of the tyrosine hydroxylase (TH), GD2 synthetase (GALGT), and embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) genes to detect disseminated neuroblastoma cells. We assessed assay sensitivity by addition experiments and then analyzed 97 neuroblastic tumor, BM, peripheral blood (PB), or peripheral blood stem cell (PBSC) samples from 30 patients. The QPCR results were compared with those of a standardized immunocytochemical assay. Results: The molecular markers were highly expressed in all evaluated tumor samples. In addition, 32%, 11%, and 38% of all BM, PB, and PBSC samples scored positive for TH, GALGT, or ELAVL4, respectively. The TH and ELAVL4 assays could detect 1 neuroblastoma cell in 106 mononuclear cells. By contrast, the GALGT QPCR assay could detect 1 neuroblastoma cell in 104 mononuclear cells. We assessed the potential prognostic value of TH, GALGT, and ELAVL4 QPCR by analyzing subsequent samples from 3 patients with stage 4 disease. Preliminary results indicated that persistence of high ELAVL4 expression has prognostic value. Conclusions: ELAVL4 QPCR can be used to detect residual neuroblastoma cells in clinical samples. However, combination of several molecular markers and screening techniques should be considered to ensure reliable detection of rare neuroblastoma cells.

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