Prospective investigation of applicability and the prognostic significance of bone marrow involvement in patients with neuroblastoma detected by quantitative reverse transcription PCR

2018 ◽  
Vol 65 (11) ◽  
pp. e27354 ◽  
Author(s):  
Alexander E. Druy ◽  
Egor V. Shorikov ◽  
Grigory A. Tsaur ◽  
Alexander M. Popov ◽  
Artem N. Zaychikov ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Reverse Transcription ◽  
Bone Marrow Involvement ◽  
Prognostic Significance ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr ◽  
Prospective Investigation

scholarly journals IFT80 Improves Invasion Ability in Gastric Cancer Cell Line via ift80/p75NGFR/MMP9 Signaling

2018 ◽  
Vol 19 (11) ◽  
pp. 3616 ◽  
Author(s):  
Rui Wang ◽  
Xiaoyan Deng ◽  
Chengfu Yuan ◽  
Hongmei Xin ◽  
Geli Liu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Reverse Transcription ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cells ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr ◽  
Gastric Cancer Cell Lines

The assembly and maintenance of cilia depend on intraflagellar transport (IFT) proteins, which play an important role in development and homeostasis. IFT80 is a newly defined IFT protein and partial mutation of IFT80 in humans causes diseases such as Jeune asphyxiating thoracic dystrophy (JATD) and short rib polydactyly (SRP) type III, both characterized by abnormal skeletal development. However, the role and mechanism of IFT80 in the invasion of gastric cancer is unknown. We established SGC-7901 and MKN-45 gastric cancer cell lines that stably overexpressed IFT80, as verified by quantitative reverse transcription-PCR, Western blot, and immunofluorescence. Matrix metalloproteinase-9 (MMP9) plays an important role in tumor invasion, and its expression was assessed by quantitative reverse transcription-PCR, Western blotting, and immunofluorescence. The invasion ability of IFT80 on SGC-7901 and MKN-45 cells was examined by the Matrigel invasion assay. The relationship between p75NGFR, and the p75NGFR antagonists, PD90780 and IFT80, were detected by quantitative reverse transcription-PCR and Western blotting. We first detected an IFT80 expression pattern, and found that IFT80 was highly expressed in gastric cancer clinical samples. Overexpression of IFT80 in the gastric cancer cell lines, SGC-7901 and MKN-45, led to lengthening cilia. Additionally, overexpression of IFT80 significantly improved proliferation and invasion, but inhibited apoptosis, in gastric cancer cells. We further found that overexpression of IFT80 increased p75NGFR and MMP9 mRNA and protein expression. Treatment with the p75NGFR antagonist PD90780 inhibited the increased invasion ability resulting from overexpression of IFT80 in SGC-7901 and MKN-45 gastric cancer cells. Thus, these results suggest that IFT80 plays an important role in invasion of gastric cancer through regulating the ift80/p75NGFR/MMP9 signal pathways.

scholarly journals Two Internal Origins of Replication in Streptomyces Linear Plasmid pFRL1

2010 ◽  
Vol 76 (17) ◽  
pp. 5676-5683 ◽  
Author(s):  
Ran Zhang ◽  
Shiyuan Peng ◽  
Zhongjun Qin
Keyword(s):  
Reverse Transcription ◽  
Northern Hybridization ◽  
Linear Plasmids ◽  
Time Points ◽  
Linear Mode ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr ◽  
Origins Of Replication ◽  
Additional Locus

ABSTRACT Previous reports showed that Streptomyces linear plasmids usually contain one internal replication locus. Here, we identified two new replication loci on pFRL1, one (rep1A-ncs1) next to a telomere and another (rep2A-ncs2) ∼10 kb from it. The rep1A-ncs1 locus was able to direct replication independently in both linear and circular modes, whereas rep2A-ncs2 required an additional locus, rlrA-rorA, in order to direct propagation in linear mode. Rep1A protein bound to ncs1 in vitro. By quantitative reverse transcription-PCR and Northern hybridization, we showed that transcription of rep1A and rep2A varied during development and that each dominated at different time points. pFRL1-derived linear plasmids were inherited through spores more stably than circular plasmids and were more stable with pSLA2 telomeres than with pFRL1 telomeres in Streptomyces lividans.

High-Throughput Real-Time Quantitative Reverse Transcription PCR

2006 ◽  
Vol 73 (1) ◽  
pp. 15.8.1-15.8.28 ◽  
Author(s):  
Angie L. Bookout ◽  
Carolyn L. Cummins ◽  
David J. Mangelsdorf ◽  
Jean M. Pesola ◽  
Martha F. Kramer
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Reverse Transcription ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

scholarly journals Multiplex, Quantitative, Reverse Transcription PCR Detection of Influenza Viruses Using Droplet Microfluidic Technology

Micromachines ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 63-79 ◽  
Author(s):  
Ravi Prakash ◽  
Kanti Pabbaraju ◽  
Sallene Wong ◽  
Anita Wong ◽  
Raymond Tellier ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Pcr Detection ◽  
Influenza Viruses ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr ◽  
Microfluidic Technology
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