scholarly journals Molecular Beacon Reverse Transcription-PCR of Human Chorionic Gonadotropin-β-3, -5, and -8 mRNAs Has Prognostic Value in Breast Cancer

2003 ◽  
Vol 49 (7) ◽  
pp. 1074-1080 ◽  
Author(s):  
Paul N Span ◽  
Peggy Manders ◽  
Joop J T M Heuvel ◽  
Chris M G Thomas ◽  
Remko R Bosch ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Chorionic Gonadotropin ◽  
Reverse Transcription ◽  
Human Chorionic Gonadotropin ◽  
Prognostic Value ◽  
Molecular Beacon ◽  
High Expression ◽  
Node Negative ◽  
Reverse Transcription Pcr ◽  
Modified Molecular Beacon

Abstract Background: The β-subunit of human chorionic gonadotropin (hCG) is encoded by four genes, of which expression of the hCGβ-3, -5, and -8 genes could have prognostic value in breast cancer. Methods: Applying a new, modified Molecular Beacon reverse transcription-PCR assay, we investigated the prognostic value of the hCGβ-3, -5, and -8 gene transcripts in 129 sporadic unilateral breast cancer samples from patients with a median follow-up of 62.3 months. Results: Expression of hCGβ-3, -5, -8 was significantly (P = 0.020) associated with relapse-free survival (RFS). In multivariate survival analysis, hCGβ-3, -5, and -8 maintained prognostic value for RFS, with high expression predicting shorter RFS (P = 0.015; hazard ratio, 2.25; 95% confidence interval, 1.17–4.34). Only 1 of 24 (4%) node-negative patients with low hCGβ-3, -5, -8 expression relapsed, in contrast to 7 of 26 (27%) patients with high expression (P = 0.046). Conclusions: Expression of hCGβ-3, -5, -8, which differ by only one nucleotide from other hCGβ genes, can be assessed by our modified Molecular Beacon assay in breast cancer tissues. Expression of hCGβ-3, -5, -8 has independent, prognostic value for RFS in breast cancer and may help identify node-negative patients with poor prognosis.

scholarly journals Human chorionic gonadotropin (hCG) and prevention of breast cancer

2007 ◽  
Vol 269 (1-2) ◽  
pp. 93-98 ◽  
Author(s):  
Jaak Ph. Janssens ◽  
José Russo ◽  
Irma Russo ◽  
Luc Michiels ◽  
Gilbert Donders ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Prevention Of Breast Cancer

scholarly journals Prognostic Value of Gene Signatures and Proliferation in Lymph-Node-Negative Breast Cancer

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90642 ◽  
Author(s):  
Kristin Jonsdottir ◽  
Jörg Assmus ◽  
Aida Slewa ◽  
Einar Gudlaugsson ◽  
Ivar Skaland ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Prognostic Value ◽  
Node Negative ◽  
Gene Signatures ◽  
Node Negative Breast Cancer

scholarly journals Comparing the Prognostic Value of PTEN and Akt Expression with the Mitotic Activity Index in Adjuvant Chemotherapy-Treated Node-Negative Breast Cancer patients aged <55 years

2007 ◽  
Vol 29 (1) ◽  
pp. 25-35
Author(s):  
Emiel A. M. Janssen ◽  
Håvard Søiland ◽  
Ivar Skaland ◽  
Einar Gudlaugson ◽  
Kjell H. Kjellevold ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cancer Patients ◽  
Mitotic Activity ◽  
Invasive Breast Cancer ◽  
Prognostic Value ◽  
Activity Index ◽  
Breast Cancer Patients ◽  
Node Negative ◽  
Mitotic Activity Index

Background: The prognostic value of the PI3K/Akt/mTOR pathway and PTEN in invasive breast cancer (IBC) is controversial. Cell proliferation, especially the Mitotic Activity Index (MAI), is strongly prognostic in lymph node-negative (LNneg) invasive breast cancer. However, its prognostic value has not been compared with the value of Akt and PTEN expression. Material and Methods: Prognostic comparison of Her2Neu, p110alpha (PIK3CA), Akt, mTOR, PTEN, MAI and cell-cycle regulators in 125 LNneg patients aged <55 years with cyclophosphamide, methotrexate, and 5-fluorouracil (CMF)-based adjuvant systemic chemotherapy. Results: Twenty-one (17%) patients developed distant metastases = DMs (median follow-up: 134 months). p110alpha correlated (p = 0.01) with pAkt but only in PTEN-negatives; pAkt correlated (p = 0.02) with mTOR. PTEN-negativity correlated with high MAI, high grade and ER-negativity (p = 0.009). The MAI was the strongest prognosticator (Hazard Ratio = HR = 2.9, p = 0.01). Her2Neu/p110α/Akt/mTOR features have no additional prognostic value to the MAI. PTEN had additional value but only in MAI < 3 (39/125 = 31%; 8% DMs). 19/39 = 49% of the MAI < 3 patients have combined MAI < 3 / PTEN+ with 0% DMs, contrasting 15% DMs in MAI < 3 / PTEN− (p = 0.03). Conclusions: In T1−3N0M0 adjuvant CMF-treated breast cancer patients aged <55 years, MAI was the strongest survival predictor. The PI3K/Akt/mTOR pathway and cell-cycle regulator characteristics had no additional prognostic value, but PTEN has. Patients with combined MAI < 3 & PTEN-positivity had 100% survival. The small subgroup of MAI < 3 patients that died were PTEN-negative.

Enhancement of Radiosensitivity of the MCF-7 Breast Cancer Cell Line with Human Chorionic Gonadotropin

2002 ◽  
Vol 72 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Sunthorn Pond-Tor ◽  
Ryan G. Rhodes ◽  
Paul E. Dahlberg ◽  
John T. Leith ◽  
John McMichael ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Chorionic Gonadotropin ◽  
Breast Cancer Cell Line ◽  
Human Chorionic Gonadotropin ◽  
Cancer Cell Line ◽  
Mcf 7

An Eco-friendly Fabrication of Silver Chloride Nanoparticles (AgCLNPs) using Onopordum acanthium L extract Induces Apoptosis in Breast Cancer MDA-MB-232 Cells

2021 ◽  
Author(s):  
Seyedeh Negin Shahcheraghi ◽  
Seyed Ataollah Sadat Shandiz ◽  
Bahareh Pakpour
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Cell Lines ◽  
Reverse Transcription ◽  
Hek293 Cell ◽  
Silver Chloride ◽  
Hek293 Cells ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Onopordum Acanthium

Abstract In the current experimental work, silver chloride nanoparticles (AgClNPs) were fabricated using Onopordum acanthium L extract and their apoptotic and cytotoxicity properties on breast cancer MDA_MB232 and normal HEK293 cell lines were also evaluated. AgClNPs formation was determined by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) profile. Effect of fabricated AgClNPs on MDA_MB232 and HEK293 cells viability was performed using colorimetric MTT assay. Alterations in the mRNA expression levels of CAD and Bax genes in MDA-MB-232 cells were done using quantitative real-time reverse transcription-PCR (qRT-PCR) method. Subsequently, apoptotic properties were determined using flow cytometry and fluorescence microscopy studies. MTT results investigated that AgCLNPs have a significant dose-dependent lethal activity on MDA_MB232 compared to HEK293 cell lines. Quantitative real-time reverse transcription-PCR (qRT-PCR) results have also shown that AgCLNPs could up-regulate the apoptotic Bax and CAD gene expressions in the MDA_MB232 cells. Additionally, apoptotic assessment was performed by cell cycle analysis, annexin V/PI test, Hoescht 33258 dye, acridine orange and ethidium bromide (AO/EB) staining along with the detection of the reactive oxygen species (ROS) generation. Our results suggest that novel silver chloride nanoparticles fabricated by Onopordum acanthium L extract can display some promising cytotoxic properties through inducing apoptosis pathway.

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