scholarly journals Simulation design of wide-field temporal-focusing multiphoton excitation with a tunable excitation wavelength

OSA Continuum ◽  
2019 ◽  
Vol 2 (4) ◽  
pp. 1174 ◽  
Author(s):  
Chi-Hsiang Lien ◽  
Chun-Yu Lin ◽  
Chia-Yuan Chang ◽  
Fan-Ching Chien
Keyword(s):  
Excitation Wavelength ◽  
Wide Field ◽  
Simulation Design ◽  
Multiphoton Excitation ◽  
Temporal Focusing

scholarly journals Wide-field three-dimensional optical imaging using temporal focusing for holographically trapped microparticles

2015 ◽  
Vol 40 (21) ◽  
pp. 4847 ◽  
Author(s):  
Roman Spesyvtsev ◽  
Helen A. Rendall ◽  
Kishan Dholakia
Keyword(s):  
Optical Imaging ◽  
Three Dimensional ◽  
Wide Field ◽  
Temporal Focusing

scholarly journals De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media

2021 ◽  
Vol 7 (28) ◽  
pp. eaay5496
Author(s):  
Cheng Zheng ◽  
Jong Kang Park ◽  
Murat Yildirim ◽  
Josiah R. Boivin ◽  
Yi Xue ◽  
...  
Keyword(s):  
High Resolution ◽  
Structural Features ◽  
Computational Imaging ◽  
Tissue Imaging ◽  
Wide Field ◽  
Scattering Media ◽  
Temporal Focusing ◽  
Field Imaging ◽  
Wide Field Imaging

Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found successes in optically cleared tissues and at shallower depths, but the scattering of emission photons in thick turbid samples severely degrades image quality at the camera. To address this challenge, we introduce a novel technique called De-scattering with Excitation Patterning or “DEEP,” which uses patterned nonlinear excitation followed by computational imaging–assisted wide-field detection. Multiphoton temporal focusing allows high-resolution excitation patterns to be projected deep inside specimen at multiple scattering lengths due to the use of long wavelength light. Computational reconstruction allows high-resolution structural features to be reconstructed from tens to hundreds of DEEP images instead of millions of point-scanning measurements.

scholarly journals Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device

2014 ◽  
Vol 5 (8) ◽  
pp. 2526 ◽  
Author(s):  
Li-Chung Cheng ◽  
Chi-Hsiang Lien ◽  
Yong Da Sie ◽  
Yvonne Yuling Hu ◽  
Chun-Yu Lin ◽  
...  
Keyword(s):  
Digital Micromirror Device ◽  
Structured Illumination ◽  
Multiphoton Excitation ◽  
Temporal Focusing

scholarly journals Two-color temporal focusing multiphoton excitation imaging with tunable-wavelength excitation

2017 ◽  
Vol 22 (2) ◽  
pp. 026008 ◽  
Author(s):  
Chi-Hsiang Lien ◽  
Gerald Abrigo ◽  
Pei-Hsuan Chen ◽  
Fan-Ching Chien
Keyword(s):  
Multiphoton Excitation ◽  
Temporal Focusing ◽  
Tunable Wavelength

scholarly journals Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography

2018 ◽  
Vol 23 (11) ◽  
pp. 1 ◽  
Author(s):  
Yong Da Sie ◽  
Chia-Yuan Chang ◽  
Chun-Yu Lin ◽  
Nan-Shan Chang ◽  
Paul J. Campagnola
Keyword(s):  
Digital Micromirror Device ◽  
Multiphoton Excitation ◽  
Temporal Focusing
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