Simultaneous spatial and temporal focusing for remote axial scanning in wide field imaging

2006 ◽  
Author(s):  
Michael Durst ◽  
Guanghao Zhu ◽  
Chris Xu
Keyword(s):  
Wide Field ◽  
Temporal Focusing ◽  
Axial Scanning ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media

2021 ◽  
Vol 7 (28) ◽  
pp. eaay5496
Author(s):  
Cheng Zheng ◽  
Jong Kang Park ◽  
Murat Yildirim ◽  
Josiah R. Boivin ◽  
Yi Xue ◽  
...  
Keyword(s):  
High Resolution ◽  
Structural Features ◽  
Computational Imaging ◽  
Tissue Imaging ◽  
Wide Field ◽  
Scattering Media ◽  
Temporal Focusing ◽  
Field Imaging ◽  
Wide Field Imaging

Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found successes in optically cleared tissues and at shallower depths, but the scattering of emission photons in thick turbid samples severely degrades image quality at the camera. To address this challenge, we introduce a novel technique called De-scattering with Excitation Patterning or “DEEP,” which uses patterned nonlinear excitation followed by computational imaging–assisted wide-field detection. Multiphoton temporal focusing allows high-resolution excitation patterns to be projected deep inside specimen at multiple scattering lengths due to the use of long wavelength light. Computational reconstruction allows high-resolution structural features to be reconstructed from tens to hundreds of DEEP images instead of millions of point-scanning measurements.

scholarly journals Camera-based localization microscopy optimized with calibrated structured illumination

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Martin Schmidt ◽  
Adam C. Hundahl ◽  
Henrik Flyvbjerg ◽  
Rodolphe Marie ◽  
Kim I. Mortensen
Keyword(s):  
Data Analysis ◽  
Super Resolution ◽  
Wide Field ◽  
Structured Illumination ◽  
Uniform Illumination ◽  
Localization Microscopy ◽  
Localization And Tracking ◽  
Field Imaging ◽  
Wide Field Imaging ◽  
Localization Information

AbstractUntil very recently, super-resolution localization and tracking of fluorescent particles used camera-based wide-field imaging with uniform illumination. Then it was demonstrated that structured illuminations encode additional localization information in images. The first demonstration of this uses scanning and hence suffers from limited throughput. This limitation was mitigated by fusing camera-based localization with wide-field structured illumination. Current implementations, however, use effectively only half the localization information that they encode in images. Here we demonstrate how all of this information may be exploited by careful calibration of the structured illumination. Our approach achieves maximal resolution for given structured illumination, has a simple data analysis, and applies to any structured illumination in principle. We demonstrate this with an only slightly modified wide-field microscope. Our protocol should boost the emerging field of high-precision localization with structured illumination.

scholarly journals Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain

2021 ◽  
Vol 2 (2) ◽  
pp. 100542
Author(s):  
Taiga Takahashi ◽  
Hong Zhang ◽  
Kohei Otomo ◽  
Yosuke Okamura ◽  
Tomomi Nemoto
Keyword(s):  
Mouse Brain ◽  
Wide Field ◽  
Cranial Window ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals The Virmos Project

1998 ◽  
Vol 11 (1) ◽  
pp. 492-492
Author(s):  
D. MacCagni ◽  
O. Le Fèvre ◽  
G. Vettolani ◽  
D. Mancini ◽  
J.P. Picat ◽  
...  
Keyword(s):  
Large Scale ◽  
Large Scale Structure ◽  
Scale Structure ◽  
Structure Evolution ◽  
Wide Field ◽  
Look Back ◽  
Field Imaging ◽  
Wide Field Imaging

Large and deep spectroscopic samples of galaxies are essential to study galaxies and large scale structure evolution out to look-back times ~ 10% the current age of the vmiverse. Keeping this scientific and observational goal in mind, we designed and are presently building two wide-field imaging spectrographs to be installed at the Nasmyth foci of the ESO-VLT Unit Telescopes 3 and 4.

scholarly journals Label Free High Speed Wide Field Imaging of Single Microtubules using Interference Reflection Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 504a-505a
Author(s):  
Mohammed Mahamdeh ◽  
Steve Simmert ◽  
Anna Łuchniak ◽  
Erik Schäffer ◽  
Jonathon Howard
Keyword(s):  
High Speed ◽  
Wide Field ◽  
Label Free ◽  
Field Imaging ◽  
Wide Field Imaging

The Near-Earth Asteroid Tracking (NEAT) Program: An Automated System for Telescope Control, Wide-Field Imaging, and Object Detection

1999 ◽  
Vol 117 (3) ◽  
pp. 1616-1633 ◽  
Author(s):  
Steven H. Pravdo ◽  
David L. Rabinowitz ◽  
Eleanor F. Helin ◽  
Kenneth J. Lawrence ◽  
Raymond J. Bambery ◽  
...  
Keyword(s):  
Object Detection ◽  
Automated System ◽  
Wide Field ◽  
Earth Asteroid ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals Chronic wide-field imaging of brain hemodynamics in behaving animals

2016 ◽  
Vol 8 (1) ◽  
pp. 436 ◽  
Author(s):  
Peng Miao ◽  
Lingke Zhang ◽  
Miao Li ◽  
Yiguang Zhang ◽  
Shihan Feng ◽  
...  
Keyword(s):  
Wide Field ◽  
Brain Hemodynamics ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals The Brazilian wide field imaging camera (WFI) for the China/Brazil earth resources satellite: CBERS 3 and 4

2018 ◽  
Author(s):  
S. H. Evangelista ◽  
A. G. Santos ◽  
L. C. N. Scaduto ◽  
E. G. Carvalho ◽  
R. G. Modugno ◽  
...  
Keyword(s):  
Wide Field ◽  
Imaging Camera ◽  
Field Imaging ◽  
Wide Field Imaging
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