scholarly journals Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device

2014 ◽  
Vol 5 (8) ◽  
pp. 2526 ◽  
Author(s):  
Li-Chung Cheng ◽  
Chi-Hsiang Lien ◽  
Yong Da Sie ◽  
Yvonne Yuling Hu ◽  
Chun-Yu Lin ◽  
...  
2018 ◽  
Vol 23 (11) ◽  
pp. 1 ◽  
Author(s):  
Yong Da Sie ◽  
Chia-Yuan Chang ◽  
Chun-Yu Lin ◽  
Nan-Shan Chang ◽  
Paul J. Campagnola

2014 ◽  
Vol 39 (11) ◽  
pp. 3134 ◽  
Author(s):  
Jenq-Nan Yih ◽  
Yvonne Yuling Hu ◽  
Yong Da Sie ◽  
Li-Chung Cheng ◽  
Chi-Hsiang Lien ◽  
...  

2021 ◽  
Vol 120 (3) ◽  
pp. 179a
Author(s):  
Peter T. Brown ◽  
Rory Kruithoff ◽  
Gregory J. Seedorf ◽  
Douglas P. Shepherd

2019 ◽  
Vol 12 (03) ◽  
pp. 1950014 ◽  
Author(s):  
Xibin Yang ◽  
Qian Zhu ◽  
Zhenglong Sun ◽  
Gang Wen ◽  
Xin Jin ◽  
...  

Structured illumination microscopy (SIM) is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly used fluorescent labeling methods. Structured illumination can be obtained by either laser interference or projection of fringe patterns. Here, we proposed a fringe projector composed of a compact multi-wavelength LEDs module and a digital micromirror device (DMD) which can be directly attached to most commercial inverted fluorescent microscopes and update it into a SIM system. The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured light field were studied. With the optimized fringe pattern, [Formula: see text] resolution improvement could be obtained with high-end oil objectives. Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated. Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in the field of life science and medicine.


2017 ◽  
Vol 56 (5) ◽  
pp. 052501 ◽  
Author(s):  
Keisuke Isobe ◽  
Keisuke Toda ◽  
Qiyuan Song ◽  
Fumihiko Kannari ◽  
Hiroyuki Kawano ◽  
...  

CLEO: 2014 ◽  
2014 ◽  
Author(s):  
Keisuke Isobe ◽  
Kyohei Mochizuki ◽  
Qiyuan Song ◽  
Akira Suda ◽  
Fumihiko Kannari ◽  
...  

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