The features of temporal focusing multiphoton excitation fluorescence microscopy using the different excitation wavelength (Conference Presentation)

2019 ◽  
Author(s):  
Fan-Ching Chien ◽  
Chun-Yu Lin ◽  
Chia-Yuan Chang ◽  
Chi-Hsiang Lien
Keyword(s):  
Fluorescence Microscopy ◽  
Excitation Wavelength ◽  
Multiphoton Excitation ◽  
Temporal Focusing

scholarly journals Simulation design of wide-field temporal-focusing multiphoton excitation with a tunable excitation wavelength

OSA Continuum ◽  
2019 ◽  
Vol 2 (4) ◽  
pp. 1174 ◽  
Author(s):  
Chi-Hsiang Lien ◽  
Chun-Yu Lin ◽  
Chia-Yuan Chang ◽  
Fan-Ching Chien
Keyword(s):  
Excitation Wavelength ◽  
Wide Field ◽  
Simulation Design ◽  
Multiphoton Excitation ◽  
Temporal Focusing

scholarly journals Scanless multitarget-matching multiphoton excitation fluorescence microscopy

2018 ◽  
Vol 11 (02) ◽  
pp. 1750013 ◽  
Author(s):  
Junpeng Qiu ◽  
Lei Wang ◽  
Bruce Zhi Gao ◽  
Junle Qu ◽  
Yonghong Shao
Keyword(s):  
Fluorescence Microscopy ◽  
Near Infrared ◽  
Objective Lens ◽  
Fluorescence Signal ◽  
Multiphoton Excitation ◽  
Light Modulator ◽  
Blazed Grating ◽  
Temporal Focusing ◽  
Excitation Pattern ◽  
Effective Use

Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved. The SLM shaped an incoming mode-locked, near-infrared Ti:sapphire laser beam into an excitation pattern with addressable shapes and sizes that matched the samples of interest in the field of view. Temporal and spatial focusing were simultaneously realized by combining an objective lens and a blazed grating. The fluorescence signal from illuminated areas was recorded by a two-dimensional sCMOS camera. Compared with a conventional temporal focusing multiphoton microscope, our microscope achieved effective use of the laser power and decreased photodamage with higher axial resolution.

scholarly journals Multiphoton excitation fluorescence microscopy in planar membrane systems

2010 ◽  
Vol 1798 (7) ◽  
pp. 1301-1308 ◽  
Author(s):  
Jonathan Brewer ◽  
Jorge Bernardino de la Serna ◽  
Kerstin Wagner ◽  
Luis A. Bagatolli
Keyword(s):  
Fluorescence Microscopy ◽  
Membrane Systems ◽  
Multiphoton Excitation ◽  
Planar Membrane

How Multiphoton Excitation can Illuminate Biophysics

1997 ◽  
Vol 3 (S2) ◽  
pp. 299-300
Author(s):  
W. W. Webb
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Infrared Light ◽  
Focal Volume ◽  
Multiphoton Excitation ◽  
Fluorescence Excitation ◽  
Photon Excitation ◽  
Rate Of Absorption ◽  
Molecular Excitation

Multiphoton molecular excitation by the strongly focused femtosecond pulses of infrared light generated as an 80 MHZ pulse train by a mode locked laser provides intrinsic submicron three dimensional spatial resolution of fluorescence excitation and photochemistry for laser scanning fluorescence microscopy. Because two-photon excitation requires simultaneous (∼10-16 seconds), absorption of two-photons focused laser intensities of about 1022 photons/cm2s are required. Since the rate of absorption is proportional to the square of the intensity, excitation is limited to the focal volume and is negligible elsewhere along the double cone of the focused illumination. Therefore, out of focus photodamage and fluorescence are generally negligible and laser scanning fluorescence microscopy with multiphoton excitation is intrinsically three dimensionally resolved with no out of focus background. Since the appropriate wave lengths are infrared for multiphoton excitation of ultraviolet or visible absorbing molecules, out of focus photodamage is eliminated. This allows imaging of useful ultraviolet absorbing indicators, vital DNA stains and autofluorescence in living cells with minimal, but not necessarily negligible, photodamage.

High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation

2010 ◽  
Author(s):  
Keisuke Isobe ◽  
Akira Suda ◽  
Hiroshi Hashimoto ◽  
Fumihiko Kannari ◽  
Hiroyuki Kawano ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Multiphoton Excitation
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