Nonlinear Optical Microscope: A History of Interplay between Technology Developments and Biomedical Sciences

2011 ◽  
Author(s):  
Peter So
Keyword(s):  
Nonlinear Optical ◽  
Optical Microscope ◽  
Biomedical Sciences ◽  
History Of

Some early history of replica techniques for electron microscopy

1992 ◽  
Vol 50 (2) ◽  
pp. 1076-1077
Author(s):  
Robert M. Fisher
Keyword(s):  
Thin Film ◽  
Electron Microscopy ◽  
Optical Microscope ◽  
Early History ◽  
Bulk Materials ◽  
Thin Sections ◽  
Transmission Electron ◽  
Reflected Light ◽  
History Of ◽  
Electron Microscopes

By 1940, a half dozen or so commercial or home-built transmission electron microscopes were in use for studies of the ultrastructure of matter. These operated at 30-60 kV and most pioneering microscopists were preoccupied with their search for electron transparent substrates to support dispersions of particulates or bacteria for TEM examination and did not contemplate studies of bulk materials. Metallurgist H. Mahl and other physical scientists, accustomed to examining etched, deformed or machined specimens by reflected light in the optical microscope, were also highly motivated to capitalize on the superior resolution of the electron microscope. Mahl originated several methods of preparing thin oxide or lacquer impressions of surfaces that were transparent in his 50 kV TEM. The utility of replication was recognized immediately and many variations on the theme, including two-step negative-positive replicas, soon appeared. Intense development of replica techniques slowed after 1955 but important advances still occur. The availability of 100 kV instruments, advent of thin film methods for metals and ceramics and microtoming of thin sections for biological specimens largely eliminated any need to resort to replicas.

Depth-of-field extension in optical imaging for rapid crystal screening

2021 ◽  
Vol 77 (4) ◽  
pp. 463-470
Author(s):  
Chen Li ◽  
Changqin Ding ◽  
Minghe Li ◽  
Jiayue Rong ◽  
Hilary Florian ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Wavelength Dependence ◽  
Optical Microscope ◽  
Field Extension ◽  
Protein Crystal ◽  
Depth Of Field ◽  
Theoretical Predictions ◽  
Second Harmonic Generation Imaging

The depth of field (DoF) was extended 2.8-fold to achieve rapid crystal screening by retrofitting a custom-designed micro-retarder array (µRA) in the optical beam path of a nonlinear optical microscope. The merits of the proposed strategy for DoF enhancement were assessed in applications of second-harmonic generation imaging of protein crystals. It was found that DoF extension increased the number of crystals detected while simultaneously reducing the number of `z-slices' required for screening. Experimental measurements of the wavelength-dependence of the extended DoF were in excellent agreement with theoretical predictions. These results provide a simple and broadly applicable approach to increase the throughput of existing nonlinear optical imaging methods for protein crystal screening.

HISTORY AND PRESENT STATE OF TRANSLATIONAL ELECTRO-OPTICAL APPLICATIONS TO MEDICINE

2008 ◽  
Vol 01 (01) ◽  
pp. 1-15
Author(s):  
BRITTON CHANCE ◽  
SHOKO NIOKA
Keyword(s):  
Breast Cancer ◽  
Cancer Detection ◽  
Heat Production ◽  
Biomedical Applications ◽  
Breast Cancer Detection ◽  
Biomedical Sciences ◽  
Personal Experiences ◽  
Infra Red ◽  
History Of ◽  
Optical Applications

This paper reviews the history of the optoelectric devices applied to biomedical sciences in 20th century. It describes history of Vacuum tubes and Spectroscopies with the author's personal experiences, especially doublebeam spectroscopy. Further, the present developments of Near Infra Red (NIR) devices are described in translational biomedical applications. It includes particulary micro optoelectronics developments and present status of NIR breast cancer detection. Lastly, intrinsic molecular biomarkers are discussed especially NIR measurements of angiogenensis, hypermetabolism and heat production for cancer detection.

scholarly journals Kuru, the First Human Prion Disease

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 232 ◽  
Author(s):  
Paweł Liberski ◽  
Agata Gajos ◽  
Beata Sikorska ◽  
Shirley Lindenbaum
Keyword(s):  
Electron Microscopy ◽  
Confocal Microscopy ◽  
Prion Disease ◽  
Amyloid Plaques ◽  
Human Medicine ◽  
Spongiform Encephalopathy ◽  
Biomedical Sciences ◽  
Human Prion Disease ◽  
History Of ◽  
Jakob Disease

Kuru, the first human prion disease was transmitted to chimpanzees by D. Carleton Gajdusek (1923–2008). In this review, we summarize the history of this seminal discovery, its anthropological background, epidemiology, clinical picture, neuropathology, and molecular genetics. We provide descriptions of electron microscopy and confocal microscopy of kuru amyloid plaques retrieved from a paraffin-embedded block of an old kuru case, named Kupenota. The discovery of kuru opened new vistas of human medicine and was pivotal in the subsequent transmission of Creutzfeldt–Jakob disease, as well as the relevance that bovine spongiform encephalopathy had for transmission to humans. The transmission of kuru was one of the greatest contributions to biomedical sciences of the 20th century.

Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope

2019 ◽  
Vol 75 (6) ◽  
pp. 485-489
Author(s):  
Sung-Ho Lee ◽  
Seung-Hyun Hong ◽  
Seung-Han Park ◽  
Boram Kim ◽  
Dong Hee Kim ◽  
...  
Keyword(s):  
Cell Division ◽  
Nonlinear Optical ◽  
Optical Microscope ◽  
Fertilized Egg

Langmuir-Blodgett Films of Squarylium Dye J-Aggregates Exhibiting Femtosecond Optical Responses

1999 ◽  
Vol 561 ◽  
Author(s):  
M. Furuki ◽  
H. Kawashima ◽  
T. Tani ◽  
L. S. Pu
Keyword(s):  
Nonlinear Optical ◽  
Layer Structure ◽  
Molecular Layer ◽  
Optical Microscope ◽  
Langmuir Films ◽  
Lb Films ◽  
Langmuir Blodgett ◽  
Lb Film ◽  
Optical Responses ◽  
J Aggregates

ABSTRACTJ-aggregates of squarylium dyes in Langmuir films have been found to exhibit highly efficient and ultrafast nonlinear optical properties. We established a novel method for making Langmuir- Blodgett (LB) films of J-aggregates with a single absorption band at 784 nm. Deposition of the LB-films was carried out under excess compression of the Langmuir films at a constant speed. Coating the surface of the LB-film with a glassy poly-perfluorocarbon was found to enhance and stabilize the formation of J-aggregates. Characterization of morphology using a near-field scanning optical microscope (NSOM) showed that this LB-film has a highly ordered structure comprising 2- dimensional domains of J-aggregates. We also observed nonlinear-optical responses from this LBfilm. Ultrafast decay of absorption change (250 fs) with quite low saturation energy (3.4 μJ/cm2•pulse) was observed in femtosecond pump-probe measurements. These results suggest highly delocalization of excited states in the J-aggregates of this LB-film with the 2-dimensional mono-molecular layer structure which initially formed in the Langmuir film.

scholarly journals Multimodal laser-scanning nonlinear optical microscope with a rapid broadband Fourier-transform coherent Raman modality

2020 ◽  
Vol 28 (14) ◽  
pp. 20794
Author(s):  
Faris Sinjab ◽  
Kazuki Hashimoto ◽  
Venkata Ramaiah Badarla ◽  
Junko Omachi ◽  
Takuro Ideguchi
Keyword(s):  
Fourier Transform ◽  
Laser Scanning ◽  
Nonlinear Optical ◽  
Optical Microscope ◽  
Coherent Raman
Sign in / Sign up

Export Citation Format

Share Document