Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope

2019 ◽  
Vol 75 (6) ◽  
pp. 485-489
Author(s):  
Sung-Ho Lee ◽  
Seung-Hyun Hong ◽  
Seung-Han Park ◽  
Boram Kim ◽  
Dong Hee Kim ◽  
...  
Keyword(s):  
Cell Division ◽  
Nonlinear Optical ◽  
Optical Microscope ◽  
Fertilized Egg

Depth-of-field extension in optical imaging for rapid crystal screening

2021 ◽  
Vol 77 (4) ◽  
pp. 463-470
Author(s):  
Chen Li ◽  
Changqin Ding ◽  
Minghe Li ◽  
Jiayue Rong ◽  
Hilary Florian ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Wavelength Dependence ◽  
Optical Microscope ◽  
Field Extension ◽  
Protein Crystal ◽  
Depth Of Field ◽  
Theoretical Predictions ◽  
Second Harmonic Generation Imaging

The depth of field (DoF) was extended 2.8-fold to achieve rapid crystal screening by retrofitting a custom-designed micro-retarder array (µRA) in the optical beam path of a nonlinear optical microscope. The merits of the proposed strategy for DoF enhancement were assessed in applications of second-harmonic generation imaging of protein crystals. It was found that DoF extension increased the number of crystals detected while simultaneously reducing the number of `z-slices' required for screening. Experimental measurements of the wavelength-dependence of the extended DoF were in excellent agreement with theoretical predictions. These results provide a simple and broadly applicable approach to increase the throughput of existing nonlinear optical imaging methods for protein crystal screening.

scholarly journals Toxicity at the cellular level of artificial synthetic flavorings

2016 ◽  
Vol 38 (3) ◽  
pp. 297
Author(s):  
Ila Monize Sousa Sales ◽  
Jussara Damascena de Oliveira ◽  
Fabelina Karollyne Silva dos Santos ◽  
Lidiane De Lima Feitoza ◽  
João Marcelo de Castro e Sousa ◽  
...  
Keyword(s):  
Cell Division ◽  
Root Meristem ◽  
Combined Treatment ◽  
Optical Microscope ◽  
Cellular Level ◽  
Combined Treatments ◽  
Division Rate ◽  
Cell Division Rate ◽  
Allium Cepa L ◽  
Genotoxic Action

 The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents cookie and tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hour using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of tutti-frutti caused no change in cell division rate. In addition, doses of both flavorings and treatments combining these solutions induced cell aberrations in a significant number of cells to the A. cepa system. Therefore, under these analytical conditions, cookie flavoring and combined doses were cytotoxic and genotoxic, and tutti-frutti flavoring, although non-cytotoxic, demonstrated genotoxic action. 

Langmuir-Blodgett Films of Squarylium Dye J-Aggregates Exhibiting Femtosecond Optical Responses

1999 ◽  
Vol 561 ◽  
Author(s):  
M. Furuki ◽  
H. Kawashima ◽  
T. Tani ◽  
L. S. Pu
Keyword(s):  
Nonlinear Optical ◽  
Layer Structure ◽  
Molecular Layer ◽  
Optical Microscope ◽  
Langmuir Films ◽  
Lb Films ◽  
Langmuir Blodgett ◽  
Lb Film ◽  
Optical Responses ◽  
J Aggregates

ABSTRACTJ-aggregates of squarylium dyes in Langmuir films have been found to exhibit highly efficient and ultrafast nonlinear optical properties. We established a novel method for making Langmuir- Blodgett (LB) films of J-aggregates with a single absorption band at 784 nm. Deposition of the LB-films was carried out under excess compression of the Langmuir films at a constant speed. Coating the surface of the LB-film with a glassy poly-perfluorocarbon was found to enhance and stabilize the formation of J-aggregates. Characterization of morphology using a near-field scanning optical microscope (NSOM) showed that this LB-film has a highly ordered structure comprising 2- dimensional domains of J-aggregates. We also observed nonlinear-optical responses from this LBfilm. Ultrafast decay of absorption change (250 fs) with quite low saturation energy (3.4 μJ/cm2•pulse) was observed in femtosecond pump-probe measurements. These results suggest highly delocalization of excited states in the J-aggregates of this LB-film with the 2-dimensional mono-molecular layer structure which initially formed in the Langmuir film.

scholarly journals Multimodal laser-scanning nonlinear optical microscope with a rapid broadband Fourier-transform coherent Raman modality

2020 ◽  
Vol 28 (14) ◽  
pp. 20794
Author(s):  
Faris Sinjab ◽  
Kazuki Hashimoto ◽  
Venkata Ramaiah Badarla ◽  
Junko Omachi ◽  
Takuro Ideguchi
Keyword(s):  
Fourier Transform ◽  
Laser Scanning ◽  
Nonlinear Optical ◽  
Optical Microscope ◽  
Coherent Raman

Scanless nonlinear optical microscope for image reconstruction and space-time correlation analysis

2017 ◽  
Author(s):  
N. G. Ceffa ◽  
F. Radaelli ◽  
P. Pozzi ◽  
M. Collini ◽  
L. Sironi ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Correlation Analysis ◽  
Nonlinear Optical ◽  
Time Correlation ◽  
Optical Microscope ◽  
Space Time
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