Multi-purpose nonlinear optical microscope. Its principle and applications to polar thin-film observation

1999 ◽  
Vol 41 (5) ◽  
pp. 688-692 ◽  
Author(s):  
Y. Uesu ◽  
N. Kato
Author(s):  
Robert M. Fisher

By 1940, a half dozen or so commercial or home-built transmission electron microscopes were in use for studies of the ultrastructure of matter. These operated at 30-60 kV and most pioneering microscopists were preoccupied with their search for electron transparent substrates to support dispersions of particulates or bacteria for TEM examination and did not contemplate studies of bulk materials. Metallurgist H. Mahl and other physical scientists, accustomed to examining etched, deformed or machined specimens by reflected light in the optical microscope, were also highly motivated to capitalize on the superior resolution of the electron microscope. Mahl originated several methods of preparing thin oxide or lacquer impressions of surfaces that were transparent in his 50 kV TEM. The utility of replication was recognized immediately and many variations on the theme, including two-step negative-positive replicas, soon appeared. Intense development of replica techniques slowed after 1955 but important advances still occur. The availability of 100 kV instruments, advent of thin film methods for metals and ceramics and microtoming of thin sections for biological specimens largely eliminated any need to resort to replicas.


2021 ◽  
Vol 77 (4) ◽  
pp. 463-470
Author(s):  
Chen Li ◽  
Changqin Ding ◽  
Minghe Li ◽  
Jiayue Rong ◽  
Hilary Florian ◽  
...  

The depth of field (DoF) was extended 2.8-fold to achieve rapid crystal screening by retrofitting a custom-designed micro-retarder array (µRA) in the optical beam path of a nonlinear optical microscope. The merits of the proposed strategy for DoF enhancement were assessed in applications of second-harmonic generation imaging of protein crystals. It was found that DoF extension increased the number of crystals detected while simultaneously reducing the number of `z-slices' required for screening. Experimental measurements of the wavelength-dependence of the extended DoF were in excellent agreement with theoretical predictions. These results provide a simple and broadly applicable approach to increase the throughput of existing nonlinear optical imaging methods for protein crystal screening.


2009 ◽  
Author(s):  
Bahareh Haji-saeed ◽  
Jed Khoury ◽  
Charles L. Woods ◽  
John Kierstead ◽  
Nasser Peyghambarian

2010 ◽  
Vol 6 (2) ◽  
pp. 121-126 ◽  
Author(s):  
Fitria Rahmawati ◽  
Sayekti Wahyuningsih ◽  
Pamularsih A.W

Thin film of TiO2 on graphite substrat has been prepared by means of chemical bath deposition. Cetyltrimethylammonium Bromide served  as linking agent of synthesized TiO2 to graphite substrate.The optical microscope and Scanning Electron Microscope (SEM) indicate that surfactant concentration affects the pore morphology of thin film Surface Area Analysis (SAA) of thin film indicated that the pore of thin film included in mesopore category. The anatase phase of TiO2 quantity arised as the surfactant concentration increase, gave high efficiency of induced photon conversion to current efficiency (% IPCE).   Keywords: thin film, TiO2, deposition, graphite


2021 ◽  
Vol 30 (4) ◽  
pp. 32-38
Author(s):  
Tharaa Sony ◽  
Thoalfiqar Zaker ◽  
Ammar Zakar ◽  
Hala Mohammed

2016 ◽  
Vol 481 ◽  
pp. 97-103 ◽  
Author(s):  
Asim Jilani ◽  
M.Sh Abdel-wahab ◽  
Attieh A. Al-ghamdi ◽  
Ammar sadik Dahlan ◽  
I.S. Yahia

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