scholarly journals Pitch-rotational manipulation of single cells and particles using single-beam thermo-optical tweezers

2020 ◽  
Vol 11 (7) ◽  
pp. 3555
Author(s):  
Sumeet Kumar ◽  
M. Gunaseelan ◽  
Rahul Vaippully ◽  
Amrendra Kumar ◽  
Mithun Ajith ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Cells ◽  
Single Beam

A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning

Lab on a Chip ◽  
2010 ◽  
Vol 10 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Emma Eriksson ◽  
Kristin Sott ◽  
Fredrik Lundqvist ◽  
Martin Sveningsson ◽  
Jan Scrimgeour ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Device ◽  
Environmental Changes ◽  
Single Cells ◽  
Cell Selection

Why Single-Beam Optical Tweezers Trap Gold Nanowires in Three Dimensions

ACS Nano ◽  
2013 ◽  
Vol 7 (10) ◽  
pp. 8794-8800 ◽  
Author(s):  
Zijie Yan ◽  
Matthew Pelton ◽  
Leonid Vigderman ◽  
Eugene R. Zubarev ◽  
Norbert F. Scherer
Keyword(s):  
Optical Tweezers ◽  
Three Dimensions ◽  
Gold Nanowires ◽  
Single Beam

scholarly journals Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers

2010 ◽  
Vol 3 (3) ◽  
pp. 213-228 ◽  
Author(s):  
Nathalie Nève ◽  
Sean S. Kohles ◽  
Shelley R. Winn ◽  
Derek C. Tretheway
Keyword(s):  
Optical Tweezers ◽  
Single Cells

scholarly journals Preferential attachment of membrane glycoproteins to the cytoskeleton at the leading edge of lamella.

1991 ◽  
Vol 114 (5) ◽  
pp. 1029-1036 ◽  
Author(s):  
D F Kucik ◽  
S C Kuo ◽  
E L Elson ◽  
M P Sheetz
Keyword(s):  
Optical Tweezers ◽  
Particle Transport ◽  
Preferential Attachment ◽  
Leading Edge ◽  
Membrane Glycoproteins ◽  
Filamentous Actin ◽  
Con A ◽  
Forward Movement ◽  
Single Beam ◽  
Apparent Diffusion

The active forward movement of cells is often associated with the rearward transport of particles over the surfaces of their lamellae. Unlike the rest of the lamella, we found that the leading edge (within 0.5 microns of the cell boundary) is specialized for rearward transport of membrane-bound particles, such as Con A-coated latex microspheres. Using a single-beam optical gradient trap (optical tweezers) to apply restraining forces to particles, we can capture, move and release particles at will. When first bound on the central lamellar surface, Con A-coated particles would diffuse randomly; when such bound particles were brought to the leading edge of the lamella with the optical tweezers, they were often transported rearward. As in our previous studies, particle transport occurred with a concurrent decrease in apparent diffusion coefficient, consistent with attachment to the cytoskeleton. For particles at the leading edge of the lamella, weak attachment to the cytoskeleton and transport occurred with a half-time of 3 s; equivalent particles elsewhere on the lamella showed no detectable attachment when monitored for several minutes. Particles held on the cell surface by the laser trap attached more strongly to the cytoskeleton with time. These particles could escape a trapping force of 0.7 X 10(-6) dyne after 18 +/- 14 (sd) s at the leading edge, and after 64 +/- 34 (SD) s elsewhere on the lamella. Fluorescent succinylated Con A staining showed no corresponding concentration of general glycoproteins at the leading edge, but cytochalasin D-resistant filamentous actin was found at the leading edge. Our results have implications for cell motility: if the forces used for rearward particle transport were applied to a rigid substratum, cells would move forward. Such a mechanism would be most efficient if the leading edge of the cell contained preferential sites for attachment and transport.

scholarly journals Construction of 3D Cellular Composites with Stem Cells Derived from Adipose Tissue and Endothelial Cells by Use of Optical Tweezers in a Natural Polymer Solution

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1759 ◽  
Author(s):  
Takehiro Yamazaki ◽  
Toshifumi Kishimoto ◽  
Paweł Leszczyński ◽  
Koichiro Sadakane ◽  
Takahiro Kenmotsu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Adipose Tissue ◽  
Optical Tweezers ◽  
Single Cells ◽  
Three Dimensional ◽  
Cell Types ◽  
Cellular Interactions ◽  
Research Fields ◽  
Wide Range

To better understand the regulation and function of cellular interactions, three-dimensional (3D) assemblies of single cells and subsequent functional analysis are gaining popularity in many research fields. While we have developed strategies to build stable cellular structures using optical tweezers in a minimally invasive state, methods for manipulating a wide range of cell types have yet to be established. To mimic organ-like structures, the construction of 3D cellular assemblies with variety of cell types is essential. Our recent studies have shown that the presence of nonspecific soluble polymers in aqueous solution is the key to creating stable 3D cellular assemblies efficiently. The present study further expands on the construction of 3D single cell assemblies using two different cell types. We have successfully generated 3D cellular assemblies, using GFP-labeled adipose tissue-derived stem cells and endothelial cells by using optical tweezers. Our findings will support the development of future applications to further characterize cellular interactions in tissue regeneration.

scholarly journals Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 308 ◽  
Author(s):  
Phalguni Tewari Kumar ◽  
Deborah Decrop ◽  
Saba Safdar ◽  
Ioannis Passaris ◽  
Tadej Kokalj ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Cell Sorting ◽  
Magnetic Beads ◽  
Single Cells ◽  
Digital Microfluidics ◽  
Cell Behavior ◽  
Bacterial Cells ◽  
Microfluidic Platforms ◽  
Dynamic Cell ◽  
Downstream Analysis

When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled methods have typically been used. However, the former, although allowing cell sorting, fails to track dynamic cell behavior, while the latter has been limited to complex channel-based microfluidic platforms. In this study, digital microfluidics (DMF) was integrated with OT for selective trapping, relocation, and further proliferation of single bacterial cells, while offering continuous imaging of cells to evaluate dynamic cell behavior. To enable this, magnetic beads coated with Salmonella Typhimurium-targeting antibodies were seeded in the microwell array of the DMF platform, and used to capture single cells of a fluorescent S. Typhimurium population. Next, OT were used to select a bead with a bacterium of interest, based on its fluorescent expression, and to relocate this bead to a different microwell on the same or different array. Using an agar patch affixed on top, the relocated bacterium was subsequently allowed to proliferate. Our OT-integrated DMF platform thus successfully enabled selective trapping, retrieval, relocation, and proliferation of bacteria of interest at single-cell level, thereby enabling their downstream analysis.

Is it possible to enlarge the trapping range of optical tweezers via a single beam?

2019 ◽  
Vol 114 (8) ◽  
pp. 081903 ◽  
Author(s):  
X. Z. Li ◽  
H. X. Ma ◽  
H. Zhang ◽  
M. M. Tang ◽  
H. H. Li ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Beam

Microfluidics Supporting an Optical Instrument for Multimodal Single Cell Biomechanics

2007 ◽  
Author(s):  
Nathalie Ne`ve ◽  
James K. Lingwood ◽  
Shelley R. Winn ◽  
Derek C. Tretheway ◽  
Sean S. Kohles
Keyword(s):  
Particle Image Velocimetry ◽  
Hydrostatic Pressure ◽  
Single Cell ◽  
Optical Tweezers ◽  
Particle Image ◽  
Single Cells ◽  
Optical Instrument ◽  
Image Velocimetry ◽  
Wide Range ◽  
Induced Stresses

Interfacing a novel micron-resolution particle image velocimetry and dual optical tweezers system (μPIVOT) with microfluidics facilitates the exposure of an individual biologic cell to a wide range of static and dynamic mechanical stress conditions. Single cells can be manipulated in a sequence of mechanical stresses (hydrostatic pressure variations, tension or compression, as well as shear and extensional fluid induced stresses) while measuring cellular deformation. The unique multimodal load states enable a new realm of single cell biomechanical studies.

Optical Tweezers: A Practical Guide

1995 ◽  
Vol 1 (2) ◽  
pp. 65-74
Author(s):  
Scot C. Kuo
Keyword(s):  
Optical Tweezers ◽  
Optical Microscope ◽  
Gradient Force ◽  
Biological Applications ◽  
Single Beam ◽  
Practical Guide ◽  
Microscopy Techniques ◽  
Displacement Measurements

Optical tweezers, or the single-beam optical gradient force trap, is becoming a major tool in biology for noninvasive micromanipulation on an optical microscope. The principles and practical aspects that influence construction are presented in an introductory primer. Quantitative theories are also reviewed but have yet to supplant user calibration. Various biological applications are summarized, including recent quantitative force and displacement measurements. Finally, tantalizing developments for new, nonimaging microscopy techniques based on optical tweezers are included.

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