Is it possible to enlarge the trapping range of optical tweezers via a single beam?

2019 ◽  
Vol 114 (8) ◽  
pp. 081903 ◽  
Author(s):  
X. Z. Li ◽  
H. X. Ma ◽  
H. Zhang ◽  
M. M. Tang ◽  
H. H. Li ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Beam

Why Single-Beam Optical Tweezers Trap Gold Nanowires in Three Dimensions

ACS Nano ◽  
2013 ◽  
Vol 7 (10) ◽  
pp. 8794-8800 ◽  
Author(s):  
Zijie Yan ◽  
Matthew Pelton ◽  
Leonid Vigderman ◽  
Eugene R. Zubarev ◽  
Norbert F. Scherer
Keyword(s):  
Optical Tweezers ◽  
Three Dimensions ◽  
Gold Nanowires ◽  
Single Beam

scholarly journals Preferential attachment of membrane glycoproteins to the cytoskeleton at the leading edge of lamella.

1991 ◽  
Vol 114 (5) ◽  
pp. 1029-1036 ◽  
Author(s):  
D F Kucik ◽  
S C Kuo ◽  
E L Elson ◽  
M P Sheetz
Keyword(s):  
Optical Tweezers ◽  
Particle Transport ◽  
Preferential Attachment ◽  
Leading Edge ◽  
Membrane Glycoproteins ◽  
Filamentous Actin ◽  
Con A ◽  
Forward Movement ◽  
Single Beam ◽  
Apparent Diffusion

The active forward movement of cells is often associated with the rearward transport of particles over the surfaces of their lamellae. Unlike the rest of the lamella, we found that the leading edge (within 0.5 microns of the cell boundary) is specialized for rearward transport of membrane-bound particles, such as Con A-coated latex microspheres. Using a single-beam optical gradient trap (optical tweezers) to apply restraining forces to particles, we can capture, move and release particles at will. When first bound on the central lamellar surface, Con A-coated particles would diffuse randomly; when such bound particles were brought to the leading edge of the lamella with the optical tweezers, they were often transported rearward. As in our previous studies, particle transport occurred with a concurrent decrease in apparent diffusion coefficient, consistent with attachment to the cytoskeleton. For particles at the leading edge of the lamella, weak attachment to the cytoskeleton and transport occurred with a half-time of 3 s; equivalent particles elsewhere on the lamella showed no detectable attachment when monitored for several minutes. Particles held on the cell surface by the laser trap attached more strongly to the cytoskeleton with time. These particles could escape a trapping force of 0.7 X 10(-6) dyne after 18 +/- 14 (sd) s at the leading edge, and after 64 +/- 34 (SD) s elsewhere on the lamella. Fluorescent succinylated Con A staining showed no corresponding concentration of general glycoproteins at the leading edge, but cytochalasin D-resistant filamentous actin was found at the leading edge. Our results have implications for cell motility: if the forces used for rearward particle transport were applied to a rigid substratum, cells would move forward. Such a mechanism would be most efficient if the leading edge of the cell contained preferential sites for attachment and transport.

Optical Tweezers: A Practical Guide

1995 ◽  
Vol 1 (2) ◽  
pp. 65-74
Author(s):  
Scot C. Kuo
Keyword(s):  
Optical Tweezers ◽  
Optical Microscope ◽  
Gradient Force ◽  
Biological Applications ◽  
Single Beam ◽  
Practical Guide ◽  
Microscopy Techniques ◽  
Displacement Measurements

Optical tweezers, or the single-beam optical gradient force trap, is becoming a major tool in biology for noninvasive micromanipulation on an optical microscope. The principles and practical aspects that influence construction are presented in an introductory primer. Quantitative theories are also reviewed but have yet to supplant user calibration. Various biological applications are summarized, including recent quantitative force and displacement measurements. Finally, tantalizing developments for new, nonimaging microscopy techniques based on optical tweezers are included.

3D controlling the bead linking to DNA molecule in a single-beam nonlinear optical tweezers

2016 ◽  
Vol 48 (12) ◽  
Author(s):  
Trung Thai Dinh ◽  
Khoa Doan Quoc ◽  
Kien Bui Xuan ◽  
Quy Ho Quang
Keyword(s):  
Optical Tweezers ◽  
Nonlinear Optical ◽  
Single Beam ◽  
Dna Molecule

Optical tweezers as a tool to study cellular function

1992 ◽  
Vol 50 (1) ◽  
pp. 424-425
Author(s):  
Steven M. Block
Keyword(s):  
Optical Tweezers ◽  
Optical Trap ◽  
Three Dimensional ◽  
Characteristic Size ◽  
Gradient Force ◽  
Single Beam ◽  
Temporal Precision ◽  
Dielectric Particles ◽  
Ligand Interactions ◽  
20 Nm

A single beam gradient force optical trap1-3, or “optical tweezers”, exerts forces on microscopic dielectric particles using a highly focused beam of laser light, and can achieve stable, three-dimensional trapping of such particles (for a review, see ref. 4). Using an infrared laser, calibratable forces in the piconewton (pN) range can be easily generated without causing significant damage to living biological specimens. Optical tweezers work through the microscope, without mechanical intrusion within sealed preparations, and can even reach directly inside transparent cells or organelles. Because it is formed by light, an optical trap can be controlled with very high spatial and temporal precision. Its characteristic size (i.e., its “grasp”) is approximately equal to the wavelength of light, but it can be used to capture and/or manipulate objects ranging in size from ∼20 nm to ∼100 mm. Biological preparations (e.g., cells, vesicles, organelles) or small particles (e.g., latex or silica microspheres, perhaps carrying reagents coupled to their surfaces) can be held, maneuvered, or released at will. Already, researchers have begun to contemplate experiments that were practically impossible just a few years ago. Some possibilities include: (1) the sorting and isolation of cells, vesicles, organelles, chromosomes, etc.; (2) the direct measurement of the mechanical properties of cytoskeletal assemblies, membranes, or membrane-bound elements; (3) measurement of the tiny forces produced by mechanoenzymes; (4) establishing cell-cell contacts, or measuring receptor-ligand interactions; (5) studying cellular rheology on the micrometer scale; (6) doing cellular microsurgery, membrane fusion, and building novel cellular (or noncellular) structures; (7) capturing and maintaining fragile biological structures away from vessel surfaces, in order to study them in isolation under optimal viewing conditions; (8) and much more! The principles by which optical tweezers work will be explained, and a videotape illustrating a number of experimental uses will be shown.

scholarly journals Pitch-rotational manipulation of single cells and particles using single-beam thermo-optical tweezers

2020 ◽  
Vol 11 (7) ◽  
pp. 3555
Author(s):  
Sumeet Kumar ◽  
M. Gunaseelan ◽  
Rahul Vaippully ◽  
Amrendra Kumar ◽  
Mithun Ajith ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Cells ◽  
Single Beam

The optical stretcher as a tool for single-particle X-ray imaging and diffraction

2018 ◽  
Vol 25 (4) ◽  
pp. 1196-1205 ◽  
Author(s):  
Jan-David Nicolas ◽  
Johannes Hagemann ◽  
Michael Sprung ◽  
Tim Salditt
Keyword(s):  
Elastic Properties ◽  
Optical Tweezers ◽  
Free Electron ◽  
High Throughput Screening ◽  
Structural Parameters ◽  
Biological Cells ◽  
X Ray Diffraction ◽  
X Ray ◽  
Single Beam ◽  
Optical Stretcher

For almost half a century, optical tweezers have successfully been used to micromanipulate micrometre and sub-micrometre-sized particles. However, in recent years it has been shown experimentally that, compared with single-beam traps, the use of two opposing and divergent laser beams can be more suitable in studying the elastic properties of biological cells and vesicles. Such a configuration is termed an optical stretcher due to its capability of applying high deforming forces on biological objects such as cells. In this article the experimental capabilities of an optical stretcher as a potential sample delivery system for X-ray diffraction and imaging studies at synchrotrons and X-ray free-electron laser (FEL) facilites are demonstrated. To highlight the potential of the optical stretcher its micromanipulation capabilities have been used to image polymer beads and label biological cells. Even in a non-optimized configuration based on a commercially available optical stretcher system, X-ray holograms could be recorded from different views on a biological cell and the three-dimensional phase of the cell could be reconstructed. The capability of the setup to deform cells at higher laser intensities in combination with, for example, X-ray diffraction studies could furthermore lead to interesting studies that couple structural parameters to elastic properties. By means of high-throughput screening, the optical stretcher could become a useful tool in X-ray studies employing synchrotron radiation, and, at a later stage, femtosecond X-ray pulses delivered by X-ray free-electron lasers.

Sign in / Sign up

Export Citation Format

Share Document