Cultivation of Chlorella vulgaris in aquaculture wastewater for protein production

2018 ◽  
Vol 11 (2) ◽  
pp. 93-100 ◽  
Author(s):  
Estefany Blanco-Carvajal ◽  
Eduar Sanchez-Galvis ◽  
Angel Dario Gonzalez-Delgado ◽  
Janet Bibiana Garcia Martinez ◽  
Andres Fernando Barajas-Solano
Keyword(s):  
Chlorella Vulgaris ◽  
Protein Production ◽  
Aquaculture Wastewater

scholarly journals Biotesting of cultivation zoocompost of Hermetia illucens larvae

2020 ◽  
Vol 28 (4) ◽  
pp. 324-335
Author(s):  
Elena N. Goncharova ◽  
Ivan R. Kurzenev ◽  
Marina I. Vasilenko ◽  
Evgeniy A. Pendyurin
Keyword(s):  
Daphnia Magna ◽  
Organic Compounds ◽  
Chlorella Vulgaris ◽  
Avena Sativa ◽  
Protein Production ◽  
Hazard Class ◽  
Hermetia Illucens ◽  
Triticum Vulgare ◽  
Test Organisms ◽  
Hermetia Illucens Larvae

A by-product of protein production from the larvae of the black soldier fly ( Hermetia illucens ) is a zoocompost consisting of a variety of organic compounds. The purpose of the work was to determine the hazard class of the zoo complex - the waste of cultivation of Hermetia illucens larvae using biotesting methods, since the use of calculated methods to determine the hazard class is difficult. Biotesting experiments were carried out on test organisms: planktonic crustacean Daphnia magna , algae Chlorella vulgaris , seeds of Avena sativa , Triticum vulgare and Hordeum sativum . It has been established that the zoocompost refers to low-risk waste. Extractions of the zoocompost stimulate the growth of plants Avena sativa and Hordeum sativum , it is supposed to be used as a phytostimulator. It has been shown that an environmentally friendly wasteless method for the production of protein additive from insects is possible.

scholarly journals Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content

Catalysts ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 573
Author(s):  
Patryk Ratomski ◽  
Małgorzata Hawrot-Paw
Keyword(s):  
Chlorophyll A ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Synthetic Medium ◽  
Nutrient Stress ◽  
Stress Conditions ◽  
Microalgal Biomass ◽  
Soxhlet Apparatus ◽  
Aquaculture Wastewater ◽  
Cellular Components

Microalgal biomass and its cellular components are used as substrates for the production of fuels. A valuable group among the components of microalgal biomass is lipids, which act as a precursor for the production of biodiesel in the transesterification process. Some methods, including the creation of stressful conditions, are applied to increase the accumulation of lipids. This study aimed to determine the effect of limited nutrient access on the growth and development of the microalga Chlorella vulgaris and the amount of lipids stored in its cells. Aquaculture wastewater (AWW) was used in the study as a source of nutrients at doses of 20%, 40%, 60%, 80% and 100%. The amount of microalgal biomass, optical density, lipid content after extraction of the biomass in Soxhlet apparatus and chlorophyll a content were determined. It was observed that the microalgae efficiently used the nutrients contained in the AWW. The largest amount of biomass was obtained in AWW80 (727 ± 19.64 mg·L−1). The OD680 (0.492 ± 0.00) determined under the same conditions was almost five times higher in AWW than in the synthetic medium. Under nutrient-stress conditions, the content of lipids in biomass ranged from 5.75% (AWW80) to 11.81% (AWW20). The highest content of chlorophyll a in microalgal cells was obtained in AWW20 (206 ± 11.33 mg∙m−3).

scholarly journals Comparison ability of algae and nanoparticles on nitrate and phosphate removal from aquaculture wastewater

2019 ◽  
Vol 6 (3) ◽  
pp. 171-177
Author(s):  
Majid Askari Hesni ◽  
Seyed Aliakbar Hedayati ◽  
Amir Qadermarzi ◽  
Mojtaba Pouladi ◽  
Somayeh Zangiabadi ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Statistical Data ◽  
Porous Plate ◽  
Phosphate Removal ◽  
Control Treatment ◽  
Laboratory System ◽  
Fish Farms ◽  
Weekly Treatment ◽  
Aquaculture Wastewater ◽  
Reactor Space

Background: Aquaculture wastewater contains high levels of phosphate and nitrate. The reuse of this water requires standards beyond the secondary standards to eliminate more organic pollutants from aquaculture effluents. In this research, the removal of these pollutants from wastewater using Chlorella vulgaris and Fe3 O4 nanoparticles in the reactor space was investigated. Methods: This study was conducted on fish farms effluent in the laboratory system. For this purpose, a 5-L semi-industrial reactor with a mixer blade, porous plate, and a compressor was designed. Chlorella vulgaris samples were collected from the natural environment and cultured in the laboratory environment. Also, Fe3 O4 nanoparticles were prepared from Iranian Nano Pishgaman Company to make the desired solution. During the experiment (3 weeks), samples were taken weekly (in one phase) from the effluent. Dissolved oxygen (DO), pH, nitrate (NO3 ), and phosphate (PO4 ) factors from the influent and effluent of the farms were measured. The statistical data were analyzed using SPSS version 21 and Excel 2013. Results: The amounts of nitrate and phosphate were decreased by about 80.76 and 80.55% in the biological reactor, whereas these amounts were 70.52 and 70.48% in the nanoparticle reactor, respectively. Also, there were significant differences in the amounts of NO3 and PO4 between the control treatment and weekly treatment (P<0.05). Conclusion: Based on the results, both reactors were able to reduce nitrate and phosphate from aquaculture wastewater, but the efficiency of the biological reactor was higher than that of the nanoparticle reactor.

scholarly journals Assessment of Antioxidant Contents and Free Radical-Scavenging Capacity of Chlorella vulgaris Cultivated in Low Cost Media

2020 ◽  
Vol 10 (23) ◽  
pp. 8611
Author(s):  
Kulwa Mtaki ◽  
Margareth S. Kyewalyanga ◽  
Matern S. P. Mtolera
Keyword(s):  
Free Radical ◽  
Chlorella Vulgaris ◽  
Low Cost ◽  
Radical Scavenging ◽  
Basal Medium ◽  
Extraction Yield ◽  
Free Radical Scavenging ◽  
Aquaculture Wastewater ◽  
Synthetic Media ◽  
Β Carotene

The current study assessed antioxidants contents (total phenolics and total flavanoids, β-carotene and lycopene) present in Chlorella vulgaris (C. vulgaris) cultivated in low cost media and their free radical scavenging activities. Microalgae was cultured using Bold basal medium (BBM) as a control, 5% banana stem compost (BCM) and aquaculture wastewater supplemented with 1.0 g/L NPK (ANM). The free radical scavenging ability was analysed using 1,1-diphenyl -2-picrylhydrazyl assay. Cells grown on BCM resulted in higher (13.73 ± 0.121%) extraction yield than in other media. The phenolic (8.53 ± 0.10 mg/g GAE) and lycopene (0.29 ± 0.008 mg/g) content were highest in cells grown on BCM and BBM, respectively. Microalgae cultured in ANM displayed higher (547.023 ± 34.703 mg/g RE) flavanoid and β-carotene (2.887 ± 0.121 mg/g) content than in other media. Furthermore, cells cultivated in BCM showed highest (97.87 ± 0.088%) scavenging activity than in BBM. These results indicated that the BCM and ANM can be used as alternative to expensive synthetic media for antioxidant production in C. vulgaris.

Bioremediation of aquaculture wastewater using microalgae Chlorella vulgaris

2017 ◽  
Vol 10 (35) ◽  
pp. 1701-1708 ◽  
Author(s):  
Estefany Blanco-Carvajal ◽  
Angel Dario Gonzalez-Delgado ◽  
Janet Bibiana Garcia-Martinez ◽  
Eduar Sanchez-Galvis ◽  
Andres Fernando Barajas-Solano
Keyword(s):  
Chlorella Vulgaris ◽  
Aquaculture Wastewater

scholarly journals Supplementing wastewater with NPK fertilizer as a cheap source of nutrients in cultivating live food (Chlorella vulgaris)

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Kulwa Mtaki ◽  
Margareth S. Kyewalyanga ◽  
Matern S. P. Mtolera
Keyword(s):  
Chlorella Vulgaris ◽  
Large Scale ◽  
Biomass Productivity ◽  
Growth Media ◽  
Potential Growth ◽  
Dry Cell Weight ◽  
Chlorophyll Protein ◽  
Aquaculture Wastewater ◽  
Large Scale Cultivation ◽  
Dry Cell

Abstract Introduction The decline in fishery resources from the wild has led to an ever increasing focus on aquaculture in recent years. With increasing aquaculture of animal species, there is an increasing need for suitable microalgae in the production of these animals. However, cultivation of microalgae in expensive pure chemical media is one of the major challenges facing large-scale cultivation of microalgae. Purpose The present study investigated the suitability of aquaculture wastewater (AWW) supplemented with NPK (nitrogen:phosphorus:potassium) fertilizer as a cheap source of nutrient to cultivate a microalga Chlorella vulgaris (C. vulgaris). Methods C. vulgaris with an initial cell density of 0.8 × 106 cells/mL was batch cultured in AWW supplemented with NPK at 0.1, 0.5, 1.0 g/L and BBM for 20 days under laboratory conditions using 2000 mL Erlenmeyer flasks. The proximate composition, chlorophyll, minerals, and vitamins analysis of C. vulgaris biomass were done using standard analytical methods. Results The highest values in optical density (4.872 ± 0.025), dry cell weight (2.858 ± 0.015 g/L), specific growth rate (0.2097 ± 0.0038 day–1), and biomass productivity (0.1701 ± 0.0007 g/L/day) were obtained in C. vulgaris grown in AWW + 1.0 NPK medium. The total chlorophyll, protein, lipid, and carbohydrate content of the microalgae biomass were in the range of 0.05–0.862%, 44.062–57.089%, 17.064–23.260%, and 15.217–21.896%, respectively. Furthermore, microalgae grown in AWW + 1.0 NPK showed good vitamin and mineral content compared to BBM grown alga. Conclusion These findings indicated that the AWW + 0.1 NPK, AWW + 0.5 NPK, and AWW + 1.0 NPK are potential growth media for C. vulgaris cultivation and can replace the BBM medium, which is very expensive and less accessible to users.

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