scholarly journals Assessment of Antioxidant Contents and Free Radical-Scavenging Capacity of Chlorella vulgaris Cultivated in Low Cost Media

2020 ◽  
Vol 10 (23) ◽  
pp. 8611
Author(s):  
Kulwa Mtaki ◽  
Margareth S. Kyewalyanga ◽  
Matern S. P. Mtolera
Keyword(s):  
Free Radical ◽  
Chlorella Vulgaris ◽  
Low Cost ◽  
Radical Scavenging ◽  
Basal Medium ◽  
Extraction Yield ◽  
Free Radical Scavenging ◽  
Aquaculture Wastewater ◽  
Synthetic Media ◽  
Β Carotene

The current study assessed antioxidants contents (total phenolics and total flavanoids, β-carotene and lycopene) present in Chlorella vulgaris (C. vulgaris) cultivated in low cost media and their free radical scavenging activities. Microalgae was cultured using Bold basal medium (BBM) as a control, 5% banana stem compost (BCM) and aquaculture wastewater supplemented with 1.0 g/L NPK (ANM). The free radical scavenging ability was analysed using 1,1-diphenyl -2-picrylhydrazyl assay. Cells grown on BCM resulted in higher (13.73 ± 0.121%) extraction yield than in other media. The phenolic (8.53 ± 0.10 mg/g GAE) and lycopene (0.29 ± 0.008 mg/g) content were highest in cells grown on BCM and BBM, respectively. Microalgae cultured in ANM displayed higher (547.023 ± 34.703 mg/g RE) flavanoid and β-carotene (2.887 ± 0.121 mg/g) content than in other media. Furthermore, cells cultivated in BCM showed highest (97.87 ± 0.088%) scavenging activity than in BBM. These results indicated that the BCM and ANM can be used as alternative to expensive synthetic media for antioxidant production in C. vulgaris.

MECHANISMS OF FREE-RADICAL SCAVENGING BY THE NUTRITIONAL ANTIOXIDANT β-CAROTENE

1995 ◽  
Vol 23 (2) ◽  
pp. 230S-230S ◽  
Author(s):  
STEVEN A. EVERETT ◽  
SUBHAS. C. KUNDU ◽  
SUSAN MADDIX ◽  
ROBIN L. WILLSON
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Β Carotene

Phytochemical, Free Radical Scavenging and Cytotoxic Assay of Cucumis Melo L. Extract and β-Carotene

2015 ◽  
Vol 2 (2) ◽  
Author(s):  
Wahyu Widowati ◽  
Rachma Micho Widyanto ◽  
Dian Ratih Laksmitawati ◽  
Pande Putu Erawijantari ◽  
Laura Wijaya ◽  
...  
Keyword(s):  
Free Radical ◽  
Cucumis Melo ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cytotoxic Assay ◽  
Cucumis Melo L ◽  
Β Carotene

scholarly journals Kandungan beta karoten, polifenol total dan aktifitas ”merantas” radikal bebas kefir susu kacang hijau (Vigna radiata) oleh pengaruh jumlah starter (Lactobacillus bulgaricus dan Candida kefir) dan konsentrasi glukosa

2014 ◽  
Vol 2 (2) ◽  
pp. 65-71
Author(s):  
Teguh Supriyono ◽  
Retno Murwani ◽  
Nurrahman Nurrahman
Keyword(s):  
Free Radical ◽  
Glucose Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Lactobacillus Bulgaricus ◽  
Scavenging Activity ◽  
Total Polyphenol ◽  
Β Carotene

Background: Kefir is a well known fermented dairy milk product which is functional for gut health. However kefir can also be made from vegetable milk such as soy or mungbean milk. Objective: To study the chemical  and functional property of Mungbean milk (MBM) Kefir Methods:Mungbean milk was fermented into MBM Kefir using Lactobacillus bulgaricus and Candida kefir. Three levels of cultures and glucose concentration were tested and a complete randomized design experiment was employed. The resulting MBM Kefir were tested for β-carotene level, total polyphenol content, and free radical scavenging activity. Beta carotene level, total polyphenols and free radicals scavenging activity were determined by spectrophotometry, Folin-Ciocalteu method, and DPPH radical scavenging assay respectively. The data were analyzed by ANOVA and when the result of ANOVA was significant, Tukey’s multiple comparison was conducted. Results: Culture concentration increased  β-carotene level (p = 0.049), total polyphenol (p = 0.015) and free radical scavenging activity (p = 0,000) of MBM kefir. Glucose concentration increased total polyphenol (p = 0.002), but had no effect on free radical scavenging activity and β-carotene level. However, there were no interaction among treatments at 0.05 significance level. Total acid level ranged from 1.57 to 2.54%, with the highest and level at 15% cultures concentration and 15% glucose concentration. Alcohol level ranged from 0.26 to 0.90% which was in accordance with the maximum standard for kefir (1%). Conclusion: An optimal product of mungbean milk kefir with highest free radical scavenging activity can be obtained using 15% cultures (Lactobacillus bulgaricus and Candida kefir) and 10% glucose concentration.

scholarly journals Synthesis of Aryl and Heterocyclic Polyenes and Their Activity in Free Radical Scavenging

2018 ◽  
Vol 42 (3) ◽  
pp. 129-132
Author(s):  
Kaini Deng ◽  
Zuxing Yang ◽  
Juan Luo ◽  
Kaiqun Wu
Keyword(s):  
Free Radical ◽  
Spectrophotometric Method ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
New Compounds ◽  
Β Carotene

Aryl polyenes and heterocyclic polyenes with the same central C20 unit of β,β-carotene but different terminal groups were synthesised. Their free radical scavenging activity was measured by a 1,1-diphenyl-2-picrylhydrazinyl spectrophotometric method. The results indicated that all the new compounds have free radical scavenging activity.

scholarly journals A Novel Isopimarane Diterpenoid with Acetylcholinesterase Inhibitory Activity from Nepeta sorgerae, an Endemic Species to the Nemrut Mountain

2012 ◽  
Vol 7 (6) ◽  
pp. 1934578X1200700
Author(s):  
Anıl Yılmaz ◽  
Pınar Çağlar ◽  
Tuncay Dirmenci ◽  
Nezhun Gören ◽  
Gülaçtı Topçu
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Endemic Species ◽  
Structure Elucidation ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Lipid Peroxidation Inhibition ◽  
Ellman’S Method ◽  
Β Carotene

From the dichloromethane extract of Nepeta sorgerae, the isolation and structure elucidation are now reported of a new isopimarane diterpenoid, named sorgerolone, and two known triterpenoids, oleanolic acid and ursolic acid. Antioxidant activity of the extracts and the isolated terpenoids was determined by the DPPH free radical scavenging and lipid peroxidation inhibition (β-carotene bleaching) methods. Anticholinesterase activity of the extracts and isolates was investigated by Ellman's method against AChE and BChE enzymes. Although the antioxidant activity results were low, the AChE enzyme inhibition of the extracts and terpenoids was very promising.

scholarly journals Sambucus racemosa L. fruit extracts obtained with conventional and deep eutectic solvents

2020 ◽  
Vol 18 (2) ◽  
pp. 89-97
Author(s):  
Vojkan Miljkovic ◽  
Biljana Djordjevic ◽  
Biljana Arsic ◽  
Marija Markovic ◽  
Ljubisa Nikolic ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Choline Chloride ◽  
Radical Scavenging ◽  
Deep Eutectic Solvents ◽  
Extraction Yield ◽  
Free Radical Scavenging ◽  
Total Phenol Content ◽  
Total Flavonoids ◽  
Fruit Extracts

This study aimed to provide information on the extraction yields, total phenols, total flavonoids, antioxidant activity, and qualitative-quantitative chemical composition of the extracts obtained from the dried fruit of Sambucus racemosa L. with conventional solvents (methanol, acetone, chloroform) and deep eutectic solvents (choline chloride urea and choline chloride glycerol). Solid-liquid extraction, Folin- Ciocalteu method, DPPH free radical scavenging, HPLC-UV/VIS were the methods used in this study. The highest extraction yield (18%) was obtained with ChCl:G as an extragent. The highest amount of total flavonoids was the content of this extract (12.3 mg QE/g d.e.). Extracts obtained with acetone and ChCl:U possess the highest amount of total phenol content (125.26 and 104.38 mg GAE/g d.e. respectively). All tested extracts showed free radical scavenging activity. Identified components of extracts are gallic acid, rutin, caftaric acid, caffeic acid, hyperoside, vitexin, and quercetin. Fruit extracts of S. racemosa are a rich source of phenols and flavonoids. The extracts obtained with DESs are more active in free radical scavenging than those obtained with conventional solvents, and possess qualitatively and quantitatively more components, apart from vitexin that was detected only in the methanol extract.

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