scholarly journals Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content

Catalysts ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 573
Author(s):  
Patryk Ratomski ◽  
Małgorzata Hawrot-Paw
Keyword(s):  
Chlorophyll A ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Synthetic Medium ◽  
Nutrient Stress ◽  
Stress Conditions ◽  
Microalgal Biomass ◽  
Soxhlet Apparatus ◽  
Aquaculture Wastewater ◽  
Cellular Components

Microalgal biomass and its cellular components are used as substrates for the production of fuels. A valuable group among the components of microalgal biomass is lipids, which act as a precursor for the production of biodiesel in the transesterification process. Some methods, including the creation of stressful conditions, are applied to increase the accumulation of lipids. This study aimed to determine the effect of limited nutrient access on the growth and development of the microalga Chlorella vulgaris and the amount of lipids stored in its cells. Aquaculture wastewater (AWW) was used in the study as a source of nutrients at doses of 20%, 40%, 60%, 80% and 100%. The amount of microalgal biomass, optical density, lipid content after extraction of the biomass in Soxhlet apparatus and chlorophyll a content were determined. It was observed that the microalgae efficiently used the nutrients contained in the AWW. The largest amount of biomass was obtained in AWW80 (727 ± 19.64 mg·L−1). The OD680 (0.492 ± 0.00) determined under the same conditions was almost five times higher in AWW than in the synthetic medium. Under nutrient-stress conditions, the content of lipids in biomass ranged from 5.75% (AWW80) to 11.81% (AWW20). The highest content of chlorophyll a in microalgal cells was obtained in AWW20 (206 ± 11.33 mg∙m−3).

scholarly journals Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 31 ◽  
Author(s):  
Maria N. Metsoviti ◽  
George Papapolymerou ◽  
Ioannis T. Karapanagiotidis ◽  
Nikolaos Katsoulas
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Growth Rates ◽  
Solar Irradiance ◽  
White Led ◽  
Microalgal Biomass ◽  
Nm Range ◽  
Effect Of Light

In this research, the effect of solar irradiance on Chlorella vulgaris cultivated in open bioreactors under greenhouse conditions was investigated, as well as of ratio of light intensity in the 420–520 nm range to light in the 580–680 nm range (I420–520/I580–680) and of artificial irradiation provided by red and white LED lamps in a closed flat plate laboratory bioreactor on the growth rate and composition. The increase in solar irradiance led to faster growth rates (μexp) of C. vulgaris under both environmental conditions studied in the greenhouse (in June up to 0.33 d−1 and in September up to 0.29 d−1) and higher lipid content in microalgal biomass (in June up to 25.6% and in September up to 24.7%). In the experiments conducted in the closed bioreactor, as the ratio I420–520/I580–680 increased, the specific growth rate and the biomass, protein and lipid productivities increased as well. Additionally, the increase in light intensity with red and white LED lamps resulted in faster growth rates (the μexp increased up to 0.36 d−1) and higher lipid content (up to 22.2%), while the protein, fiber, ash and moisture content remained relatively constant. Overall, the trend in biomass, lipid, and protein productivities as a function of light intensity was similar in the two systems (greenhouse and bioreactor).

scholarly journals Production of Microalgal Biomass Using Aquaculture Wastewater as Growth Medium

Water ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 106 ◽  
Author(s):  
Małgorzata Hawrot-Paw ◽  
Adam Koniuszy ◽  
Małgorzata Gałczyńska ◽  
Grzegorz Zając ◽  
Joanna Szyszlak-Bargłowicz
Keyword(s):  
Wastewater Treatment ◽  
Environmental Pollution ◽  
Lipid Content ◽  
Biomass Concentration ◽  
Huge Amount ◽  
Initial Value ◽  
Nitrogen And Phosphorus ◽  
Microalgal Biomass ◽  
High Lipid ◽  
Aquaculture Wastewater

Aquaculture wastewater contains a huge amount of substances that can cause environmental pollution. However, microalgae can absorb these compounds and convert them into useful biomass. In this study, Chlorella minutissima was grown in the wastewater resulting from saline aquaculture. The microalgae were found to effectively utilize nitrogen and phosphorus in the wastewater for its growth. During wastewater treatment, the cell density increased almost fivefold compared to the initial value (OD680 0.502). Moreover, batch culture resulted in the maximum biomass concentration and productivity of 4.77 g/L and 0.55 g/L/day, respectively. The contents of total nitrogen and total phosphorus in wastewater decreased by 88% and over 99%, respectively. In addition, the content of N-NO3 was reduced by 88.6%, N-NO2 by 74.3%, and dissolved orthophosphates (V) by 99%. At the beginning and throughout the experiment, the content of N-NH4 in wastewater remained below 0.05 mg/L. Furthermore, a high lipid content of 46.4% (w/w) was also obtained from the studied microalgae.

scholarly journals Proteome Analysis for Understanding Abiotic Stress (Salinity and Drought) Tolerance in Date Palm (Phoenix dactyliferaL.)

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Abdulrahman L. Al-Malki ◽  
Khalid O. Abulnaja ◽  
Wolfgang Rohde
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Date Palm ◽  
Light Harvesting ◽  
Binding Protein ◽  
Proteome Analysis ◽  
Rubisco Activase ◽  
Stress Conditions ◽  
Spectrometric Analysis ◽  
Salt And Drought Stress

This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar “Sagie” were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

scholarly journals PHYSIOLOGICAL AND BIOCHEMICAL CHANGES IN ALGAL CULTURES OF CHLORELLA VULGARIS AND SYNECHOCYSTIS SALINA (MESOPHILIC AND ANTARCTIC ISOLATES) OCCURING AFTER TREATMENT WITH UV-B RADIATION

2017 ◽  
pp. 73-82
Author(s):  
Dilyana Doneva ◽  
Juliana Ivanova ◽  
Lyudmila Kabaivanova
Keyword(s):  
Oxidative Stress ◽  
Chlorophyll A ◽  
Chlorella Vulgaris ◽  
Markers Of Oxidative Stress ◽  
Protective Strategies ◽  
The Antarctic ◽  
Antarctic Algae ◽  
Β Carotene ◽  
Stimulation Of

Determination of biomass production and viability of algal cells of Chlorella vulgaris and Synechocystis salina exposed to UV-B radiation were carried out in this study together with comparison of the mesophilic and antarctic isolates of both investigated strains. Estimation of the content of the pigments: chlorophyll a, chlorophyll b, β-carotene, C-phycocyanin and allo-phycocyanin in algal cells exposed to UV-B radiation was also accomplished. The obtained results showed that the antarctic algae are more resistant to oxidative stress than their mesophilic counterparts. The antarctic isolates of Ch. vulgaris and S. salina compared with the mesophilic ones - up to 72 h showed tolerance to low exposures of radiation, expressed in a slight stimulation of growth and viability of the cells. Antarctic isolates also showed greater resistance to low doses of UV-B radiation manifested by stimulation of the synthesis of chlorophyll a and β-carotene. The registered increase in the amount of C- and allo-phycocyanin in antarctic isolates of S. salina showed that they had developed protective strategies against UV-B radiation by increasing the concentration of the phycobiliproteins. As a result of increased UV-B background, in antarctic isolates, stronger antioxidant defence mechanisms are triggered, which proved the possibility of using them as markers of oxidative stress.

scholarly journals Study of Chlorella vulgaris sedimentation process

2018 ◽  
Vol 240 ◽  
pp. 05023 ◽  
Author(s):  
Agnieszka Patyna ◽  
Małgorzata Płaczek ◽  
Stanisław Witczak
Keyword(s):  
Chlorella Vulgaris ◽  
Dry Mass ◽  
Separation Process ◽  
Cultivation Condition ◽  
Microalgal Biomass ◽  
Algae Biomass ◽  
Algae Cultivation ◽  
Sedimentation Process ◽  
Microalgae Harvesting

The paper reports the results of Chlorella vulgaris sedimentation process including description of cultivation condition of microalgal biomass. The process of algae cultivation was carried out in photobioreactor comprising systems of carbon dioxide supply, mixing and artificial LED illumination. The growth of microalgae was determined alternatively in three ways by measuring the amount of dry mass over time, counting the cells and measurement of optical density by use of a spectrophotometer. Algae biomass with different concentration was subjected to the separation process by gravity. This led to the determination of the characteristic of sedimentation process for different concentrations and cell sizes. The experimental results indicate that sedimentation process offers a tool with a potential application for microalgae harvesting.

The Use of Light Spectra to Improve the Growth and Lipid Content of Chlorella vulgaris for Biofuels Production

2020 ◽  
Vol 13 (2) ◽  
pp. 487-498 ◽  
Author(s):  
M. del Pilar Sánchez-Saavedra ◽  
Daniel Sauceda-Carvajal ◽  
Fátima Y. Castro-Ochoa ◽  
Ceres A. Molina-Cárdenas
Keyword(s):  
Chlorella Vulgaris ◽  
Lipid Content ◽  
Light Spectra

scholarly journals Flow behavior analysis of Chlorella Vulgaris microalgal biomass

Heliyon ◽  
2019 ◽  
Vol 5 (6) ◽  
pp. e01845
Author(s):  
Suresh Kumar Yatirajula ◽  
Anuj Shrivastava ◽  
Vinod Kumar Saxena ◽  
Jagadeeshwar Kodavaty
Keyword(s):  
Behavior Analysis ◽  
Chlorella Vulgaris ◽  
Flow Behavior ◽  
Microalgal Biomass

Ultrastructure and lipid changes in Pyrenochaeta terrestris during aging

1973 ◽  
Vol 19 (4) ◽  
pp. 491-496 ◽  
Author(s):  
M. Gunasekaran ◽  
W. M. Hess ◽  
D. J. Weber
Keyword(s):  
Fatty Acids ◽  
Lipid Content ◽  
Lipid Analysis ◽  
Synthetic Medium ◽  
Growth Period ◽  
Ultrastructural Level ◽  
Dense Substance ◽  
Total Fatty Acids ◽  
Total Growth ◽  
Pyrenochaeta Terrestris

Pyrenochaeta terrestris, the onion pink root fungus, was grown on a reciprocal shaker in a synthetic medium which contained cellulose as the only carbon source. The mycelium was processed for lipid analysis and ultrastructural investigations after 8, 16, 24, and 32-day growth intervals.Hyphal cells contained membrane complexes. An electron-dense substance was present in large quantities on hyphal cell walls until after the 24-day period. As the cultures aged, organelles in some hyphal cells disintegrated and viable hyphal cells grew inside senescent cells in some cases. It was not possible to correlate positively lipid content observed at the ultrastructural level with the biochemical lipid analysis as a result of the relatively small amount of lipid observed in hyphal cells at the ultrastructural level.The lipid analysis indicated that the lipid content and mycelial weight reached a maximum at the 16-day period. The major fatty acids present were: C16:0, C18:0, C18:1, and C18:2. As aging occurred, the amount of unsaturation increased in the free fatty acids, the total fatty acids, and the fatty acids of the diglycerides and triglycerides. Only the fatty acids of the monoglycerides failed to increase in unsaturation. The sterol content increased slightly over the total growth period.

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