Age changes of the lymphatic channel of amucous membrane ofavagina

2016 ◽  
Vol 21 (32) ◽  
pp. 39-41
Author(s):  
Бураева ◽  
Zarina Buraeva ◽  
Каркусова ◽  
Alla Karkusova
Keyword(s):  
Lymphatic Vessels ◽  
Volume Density ◽  
Vaginal Mucosa ◽  
Age Changes ◽  
Lymphatic Channel

The results of researchof the lymphatic channel of the vaginal mucosa, of 28 women in the aged aspect were presented. The method of interstitial injection of lymphatic bed of Gerota blue mass revealed that post-climacteric period revealed rare, randomly arranged lymphatic capillaries and lymphatic vessels are severely deformed, unrelated general architectonics, as well as a decrease of volume density of lymphatic channel occursin the process of involution and may indicate the violation of drainage function of lymphatic device.

scholarly journals Age Changes in Cerebellar Oligodendrocytes: The Appearance of Nuclear Filaments and Increase in the Volume Density of the Nucleus and in the Number of Dark Cell Forms.

1995 ◽  
Vol 58 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Rogério A. F. MONTEIRO ◽  
Luís E. C. CONCEIÇÃO ◽  
Eduardo ROCHA ◽  
Mário M. MARINI-ABREU
Keyword(s):  
Volume Density ◽  
Age Changes ◽  
Dark Cell

Comparative characteristic of lymphatic channels of the regional lymphatic nodes in the small pelvis of women during the reproductive and postmenopausal periods

2015 ◽  
Vol 21 (31) ◽  
pp. 48-50
Author(s):  
Бураева ◽  
Zarina Buraeva
Keyword(s):  
Volume Density ◽  
The State ◽  
Comparative Characteristic ◽  
Lymphatic Channel

The results present the investigation of the state of the lymphatic channel of the regional lymphatic notes of the small pelvis of the 36 women at the aged aspect. With the use of all histologic methods with subsequent stereometric assessment of volume density of the lymphatic sinuses of lymphatic nodes, it is shown that the drainage function of the lymphatic nodes in post-climacteric period was much lower, than in women of childbearing period.

scholarly journals Pathogenic variant in EPHB4 results in central conducting lymphatic anomaly

2018 ◽  
Vol 27 (18) ◽  
pp. 3233-3245 ◽  
Author(s):  
Dong Li ◽  
Tara L Wenger ◽  
Christoph Seiler ◽  
Michael E March ◽  
Alvaro Gutierrez-Uzquiza ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Donor Site ◽  
Lymphatic Vessels ◽  
Immunoblot Analysis ◽  
Mapk Signaling ◽  
Heterozygous Mutation ◽  
Normal Donor ◽  
Loss Of Function ◽  
Lymphatic Channel ◽  
Therapeutic Benefits

Abstract Central conducting lymphatic anomaly (CCLA) is one of the complex lymphatic anomalies characterized by dilated lymphatic channels, lymphatic channel dysmotility and distal obstruction affecting lymphatic drainage. We performed whole exome sequencing (WES) of DNA from a four-generation pedigree and examined the consequences of the variant by transfection of mammalian cells and morpholino and rescue studies in zebrafish. WES revealed a heterozygous mutation in EPHB4 (RefSeq NM_004444.4; c.2334 + 1G>C) and RNA-Seq demonstrated that the EPHB4 mutation destroys the normal donor site, which leads to the use of a cryptic splice donor that results in retention of the intervening 12-bp intron sequence. Transient co-expression of the wild-type and mutant EPHB4 proteins showed reduced phosphorylation of tyrosine, consistent with a loss-of-function effect. Zebrafish ephb4a morpholino resulted in vessel misbranching and deformities in the lymphatic vessel development, indicative of possible differentiation defects in lymphatic vessels, mimicking the lymphatic presentations of the patients. Immunoblot analysis using zebrafish lysates demonstrated over-activation of mTORC1 as a consequence of reduced EPHB4 signaling. Strikingly, drugs that inhibit mTOR signaling or RAS-MAPK signaling effectively rescued the misbranching phenotype in a comparable manner. Moreover, knock-in of EPHB4 mutation in HEK293T cells also induced mTORC1 activity. Our data demonstrate the pathogenicity of the identified EPHB4 mutation as a novel cause of CCLA and suggesting that ERK inhibitors may have therapeutic benefits in such patients with complex lymphatic anomalies.

scholarly journals Identification of lymphatic pathway involved in the spread of bladder cancer: Evidence obtained from fluorescence navigation with intraoperatively injected indocyanine green

2013 ◽  
Vol 7 (5-6) ◽  
pp. 322 ◽  
Author(s):  
Shogo Inoue ◽  
Hiroaki Shiina ◽  
Yozo Mitsui ◽  
Hiroaki Yasumoto ◽  
Akio Matsubara ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Radical Cystectomy ◽  
Fluorescence Intensity ◽  
Ex Vivo ◽  
Lymphatic Vessels ◽  
Rational Approach ◽  
Lymphatic Channel ◽  
Internal Iliac ◽  
Lymphatic Pathway

Introduction: We identify lymphatic vessels draining from the bladderby using fluorescence navigation (FN) system.Methods: In total, 12 candidates for radical cystectomy and pelviclymph node dissection (PLND) were included in this study. Afteran indocyanine green (ICG) solution was injected into the bladderduring radical cystectomy, lymphatic vessels draining from thebladder were analyzed using a FN system. PLND was based onthe lymphatic mapping created from the FN measurements (in vivoprobing) in the external iliac, obturator and internal iliac regions;after PLND, the fluorescence of the removed lymph nodes (LNs)was analyzed on the bench (ex vivo probing).Results: There were no patients with complications associated withthe intravesical ICG injection. A lymphatic pathway along inferiorvesical vessels to internal iliac LNs was clearly illustrated in 7 cases.Under in-vivo probing, the fluorescence intensity of internal iliacnodes was greater than that of external iliac or obturator nodes.Under ex-vivo probing, the fluorescence intensity of internal iliacand obturator nodes was greater than that of external iliac nodes.Conclusions: Using an FN system after injecting ICG during a radicalcystectomy operation is a safe and rational approach to detectingthe lymphatic channel draining from the bladder.

scholarly journals До особливостей лімфатичного русла травної трубки дрібної рогатої худоби

2015 ◽  
pp. 47-50
Author(s):  
Н. Б. Колич ◽  
М. В. Скрипка
Keyword(s):  
Lymph Node ◽  
Oral Cavity ◽  
Lymph Nodes ◽  
Mucous Membrane ◽  
Lymphatic Vessels ◽  
Capillary Network ◽  
Regional Lymph Nodes ◽  
Lymphatic Channel ◽  
Pancreatic Lymph Node ◽  
Different Parts

Від слизової оболонки різних ділянок ротової порожнини відтік лімфи здійснюється поверхневим і глибокими лімфатичними судинами в реґіонарні лімфовузли. Слизова та м’язова оболонки передшлунків має добре розвинене лімфатичне русло, що складається зі звивистих капілярів зі сліпими виростами. У серозній оболонці розташовуються лімфатичні капіляри з колбоподібними виступами. Капіляри, з'єднуючись між собою, формують капілярну сітку. Відтік лімфи з реґіонарних лімфатичних вузлів книжки до 70 % завершуються в передшлунковому лімфатичному вузлі. From the mucous membrane of the different parts of oral cavity the outflow of lymph is carried out by superficial and deep lymphatic vessels to regional lymph nodes. The mucous and muscular membranes of proventriculus have well developed lymphatic channel, consisting of meandering capillaries with blind outgrowths. The lymphatic capillaries with flask-shaped protrusions are located in serosa. The capillaries, interconnecting with each other, form a capillary network. In 70 % of cases the outflow of lymph from the omasum regional lymph nodes is completed in pancreatic lymph node.

scholarly journals Identification of lymphatic pathway involved in the spreading of prostate cancer by fluorescence navigation approach with intraoperatively injected indocyanine green

2013 ◽  
Vol 5 (4) ◽  
pp. 254
Author(s):  
Shogo Inoue ◽  
Hiroaki Shiina ◽  
Naoko Arichi ◽  
Yozo Mitsui ◽  
Takeo Hiraoka ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Ex Vivo ◽  
Lymphatic Vessels ◽  
Lymphatic Channel ◽  
De Se ◽  
Internal Iliac ◽  
Green Solution ◽  
Vaisseaux Lymphatiques ◽  
Lymphatic Pathway

Objective: The objective of this study was to identify lymphaticvessels draining from the prostate by using a fluorescence navigation(FN) system.Methods: Fourteen subjects were candidates for radical retropubicprostatectomy (RRP) and pelvic lymph node dissection (PLND).After an indocyanine green solution was injected into the prostateduring RRP, lymphatic vessels draining from the prostate were analyzedusing a FN system. After PLND based on lymphatic mappingby the FN system (in vivo probing) was performed in the externaliliac, obturator and internal iliac regions; the fluorescence of theremoved lymph nodes (LNs) was analyzed on the bench (ex vivoprobing).Results: Under in vivo and ex vivo probing, the fluorescence intensityof internal iliac nodes was greater than that of external iliacor obturator nodes.Conclusion: The current study suggests that using a FN systemafter injecting indocyanine green is a safe and rational approachfor detecting the lymphatic channel draining from the prostate.The major lymphatic pathway involved in the spreading of prostatecancer appears to relate to internal iliac LNs, which wouldmean that the standard PLND covering external iliac and obturatorregions would not keep the cancer from spreading.Objectif : L’objectif de l’étude était de repérer les vaisseaux lymphatiquesquittant la prostate à l’aide d’un système d’imagerie parfluorescence (IF).Méthodologie : Quatorze sujets devaient subir une prostatectomieradicale rétropubienne (PRR) et une lymphadénectomie pelvienne.Après injection d’une solution de vert d’indocyanine dansla prostate pendant la PRR, les vaisseaux lymphatiques drainant laprostate ont été analysés par IF. Une lymphadénectomie pelviennefondée sur la cartographie lymphatique par IF (exploration in vivo)a ensuite été réalisée dans les régions de la fosse iliaque externe,de l’obturateur et de la fosse iliaque interne; la fluorescence desganglions lymphatiques retirés a été analysée sans délai (explorationex vivo).Résultats : Lors de l’exploration in vivo et ex vivo, l’intensité dela fluorescence des ganglions iliaques internes était plus forte quecelle des ganglions iliaques externes ou des ganglions obturateurs.Conclusion : Cette étude porte à croire que l’IF après injectionde vert d’indocyanine est une méthode sûre et rationnelle pourrepérer les vaisseaux lymphatiques drainant la prostate. La principalevoie lymphatique de propagation du cancer de la prostatesemble être reliée aux ganglions lymphatiques iliaques internes, cequi signifie que la lymphadénectomie pelvienne standard retirantles ganglions iliaques externes et obturateurs n’empêcherait pasle cancer de se propager.

scholarly journals D2-40 Immunoreactivity of Lymphatic Vessels Endothelium and Representation of Lymphatic Vessels in the Liver of Human Fetuses of Different Gestational Age

2017 ◽  
Vol 34 (1) ◽  
pp. 23-33
Author(s):  
Marko Jović ◽  
Ivan Nikolić ◽  
Vera Todorović ◽  
Aleksandar Petrović ◽  
Vladimir Petrović ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lymphatic Vessels ◽  
Volume Density ◽  
Numerical Density ◽  
Human Fetuses ◽  
Second Trimester ◽  
Third Trimester ◽  
The Third ◽  
Liver Capsule ◽  
Lymph Vessels

Summary In the reference literature, there are a few studies on the development of the lymphatic system in the liver, especially human. This study aims to establish the presence, time of appearance, distribution and representation of expression D2-40 molecule – a marker of lymph vessels endothelial cells during the fetal period of the human liver development. The livers obtained from 20 human fetuses (10 male and 12 female), aged 12-37 gestational weeks, constituted our study material. Paraffin sections, 4 µm thick, were stained with hematoxylin and eosin for histological analysis, and with LSAB2/HRP method for immunohistochemistry using the D2-40 monoclonal antibody to mark lymphatic endothelial cells. The presence of lymphatic vessels was determined by morphometry, calculating their numerical and volume density. The study showed that expression of D2-40 molecule was absent in the liver lymphatic vessels in the first trimester of development, while in the second trimester intensive D2-40 immunoreactivity was observed in the lymph vessels of the liver capsule, and low D2-40 immunopositivity of the lymph vessels in large portal spaces. In the third trimester, intensive D2-40 immunoreactivity was observed in the lymph vessels of the liver capsule and in the endothelium of numerous lymphatic vessels of various shape and size, located in the smaller and larger portal areas. Volume and numerical density of lymphatic vessels in the portal areas of the liver during fetal development increased from the second to the third trimester of pregnancy, which was proportional to the increase in volume density of the hepatic portal spaces. Based on the obtained results, a conclusion may be drawn that the lymph vessels in the liver can be identified in the first half of the second trimester, and their number was growing proportionally by the end of pregnancy.

Electron microscope study of the microgranules in human vaginal epithelium

1978 ◽  
Vol 36 (2) ◽  
pp. 568-569
Author(s):  
A. Campos ◽  
J. Vilches ◽  
J. Gomez
Keyword(s):  
Electron Microscope ◽  
Microscope Study ◽  
Intercellular Space ◽  
Electron Microscope Study ◽  
Vaginal Epithelium ◽  
Vaginal Mucosa ◽  
Secretory Phase ◽  
Cervical Epithelium ◽  
Keratinized Epithelium ◽  
Fertile Women

Microgranules have been described with different names in keratinized and in nonkeratinized epithelium. In keratinized epithelium it seems clear that the microgranules are lamellated bodies bounded by a membrane which empty their contents into the intercellular space. Their existence in nonkeratinized epithelium is more debatable. Until now the so-called microgranules have been described in nonkeratinized bucal, lingual and cervical epithelium. In the present work we describe the morphology and nature of such structures in human vaginal epithelium.Biopsies from the midlevel of the vaginal mucosa were taken from voluntary fertile women. The specimens were divided into three groups with four vaginal specimens. The first group was obtained in the folicular phase; those of the second in the postovulatory phase and, finally, the last group corresponded to the secretory phase.

A New System For Optomanual Semiautomatic Quantitative Image Analysis

1977 ◽  
Vol 35 ◽  
pp. 210-211
Author(s):  
H.P. Rohr
Keyword(s):  
Image Analysis ◽  
Volume Density ◽  
List Type ◽  
Distribution Analysis ◽  
Type Number ◽  
Point Counting ◽  
Human Eye ◽  
Quantitative Image ◽  
Point Density ◽  
Electronic Counting

Today, in image analysis the broadest possible rationalization and economization have become desirable. Basically, there are two approaches for image analysis: The image analysis through the so-called scanning methods which are usually performed without the human eye and the systems of optical semiautomatic analysis completely relying on the human eye.The new MOP AM 01 opto-manual system (fig.) represents one of the very promising approaches in this field. The instrument consists of an electronic counting and storing unit, which incorporates a microprocessor and a keyboard for choice of measuring parameters, well designed for easy use.Using the MOP AM 01 there are three possibilities of image analysis:the manual point counting,the opto-manual point counting andthe measurement of absolute areas and/or length (size distribution analysis included).To determine a point density for the calculation of the corresponding volume density the intercepts lying within the structure are scanned with the light pen.

The ultrastructure of acinar cells in the external orbital gland of young and old male rats

1978 ◽  
Vol 36 (2) ◽  
pp. 276-277
Author(s):  
R. Carriere
Keyword(s):  
Young Adulthood ◽  
Acinar Cells ◽  
Cell Number ◽  
Male Rats ◽  
Polyploid Cell ◽  
Secretory Cells ◽  
Age Changes ◽  
Albino Rat ◽  
Golgi Membranes ◽  
Diploid Cells

The external orbital gland of the albino rat exhibits both sexual dimorphism and histological age changes. In males, many cells attain a remarkable degree of polyploidy and an increase of polyploid cell number constitutes the major age change until young adulthood. The acini of young adults have a small lumen and are composed of tall serous cells. Subsequently, many acini acquire a larger lumen with an irregular outline while numerous vacuoles accumulate throughout the secretory cells. At the same time, vesicular acini with a large lumen surrounded by pale-staining low cuboidal diploid cells begin to appear and their number increases throughout old age. The fine structure of external orbital glands from both sexes has been explored and in considering acinar cells from males, emphasis was given to the form of the Golgi membranes and to nuclear infoldings of cytoplasmic constituents.

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