scholarly journals Age Changes in Cerebellar Oligodendrocytes: The Appearance of Nuclear Filaments and Increase in the Volume Density of the Nucleus and in the Number of Dark Cell Forms.

1995 ◽  
Vol 58 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Rogério A. F. MONTEIRO ◽  
Luís E. C. CONCEIÇÃO ◽  
Eduardo ROCHA ◽  
Mário M. MARINI-ABREU
Keyword(s):  
2016 ◽  
Vol 21 (32) ◽  
pp. 39-41
Author(s):  
Бураева ◽  
Zarina Buraeva ◽  
Каркусова ◽  
Alla Karkusova

The results of researchof the lymphatic channel of the vaginal mucosa, of 28 women in the aged aspect were presented. The method of interstitial injection of lymphatic bed of Gerota blue mass revealed that post-climacteric period revealed rare, randomly arranged lymphatic capillaries and lymphatic vessels are severely deformed, unrelated general architectonics, as well as a decrease of volume density of lymphatic channel occursin the process of involution and may indicate the violation of drainage function of lymphatic device.


Author(s):  
H.P. Rohr

Today, in image analysis the broadest possible rationalization and economization have become desirable. Basically, there are two approaches for image analysis: The image analysis through the so-called scanning methods which are usually performed without the human eye and the systems of optical semiautomatic analysis completely relying on the human eye.The new MOP AM 01 opto-manual system (fig.) represents one of the very promising approaches in this field. The instrument consists of an electronic counting and storing unit, which incorporates a microprocessor and a keyboard for choice of measuring parameters, well designed for easy use.Using the MOP AM 01 there are three possibilities of image analysis:the manual point counting,the opto-manual point counting andthe measurement of absolute areas and/or length (size distribution analysis included).To determine a point density for the calculation of the corresponding volume density the intercepts lying within the structure are scanned with the light pen.


Author(s):  
R. Carriere

The external orbital gland of the albino rat exhibits both sexual dimorphism and histological age changes. In males, many cells attain a remarkable degree of polyploidy and an increase of polyploid cell number constitutes the major age change until young adulthood. The acini of young adults have a small lumen and are composed of tall serous cells. Subsequently, many acini acquire a larger lumen with an irregular outline while numerous vacuoles accumulate throughout the secretory cells. At the same time, vesicular acini with a large lumen surrounded by pale-staining low cuboidal diploid cells begin to appear and their number increases throughout old age. The fine structure of external orbital glands from both sexes has been explored and in considering acinar cells from males, emphasis was given to the form of the Golgi membranes and to nuclear infoldings of cytoplasmic constituents.


Author(s):  
J. Edie

In TEM image formation, the observed contrast variations within thin sections result from differential electron scattering within microregions of varying mass thickness. It is possible to utilize these electron scattering properties to obtain objective information regarding various specimen parameters (1, 2, 3).A pragmatic, empirical approach is described which enables a microscopist to perform physical measurements of thickness of thin sections and estimates of local mass, volume, density and, possibly, molecular configurations within thin sections directly in the microscope. A Faraday cage monitors the transmitted electron beam and permits measurements of electron beam intensities.


Author(s):  
John M. Basgen ◽  
Eileen N. Ellis ◽  
S. Michael Mauer ◽  
Michael W. Steffes

To determine the efficiency of methods of quantitation of the volume density of components within kidney biopsies, techniques involving a semi-automatic digitizing tablet and stereological point counting were compared.Volume density (Vv) is a parameter reflecting the volume of a component to the volume that contains the component, e.g., the fraction of cell volume that is made up of mitochondrial volume. The units of Vv are μm3 /μm3.Kidney biopsies from 15 patients were used. Five were donor biopsies performed at the time of kidney transplantation (patients 1-5, TABLE 1) and were considered normal kidney tissue. The remaining biopsies were obtained from diabetic patients with a spectrum of diabetic kidney lesions. The biopsy specimens were fixed and embedded according to routine electron microscogy protocols. Three glomeruli from each patient were selected randomly for electron microscopy. An average of 12 unbiased and systematic micrographs were obtained from each glomerulus and printed at a final magnification of x18,000.


Author(s):  
D.C. Dominguez ◽  
J.T. Ellzey

Peroxisomes which participate in 1ipid metabolism have been shown to be altered in several metabolic disorders and toxic conditions. In alcoholic liver disease, the single lesion most frequently found is lipid accumu1ation in hepatocytes. However, the mechanisms for this 1ipid accumu1ation are not clear. The occurrence of modifications of liver peroxisomes due to excess alcohol consumption has not been subjected to a controlled study. We utilized a combination of cytochemica1 and morphometrictechniques to study the size and number of liver peroxisomes in rats fed an alcohol-supplemented diet compared to those of matched-paired control animals.Male Sprague-Daw1ey rats (400-500 g) received a liquid diet. The experimental group (N = 5/group) was fed a diet containing 30% ethanol-derived calories (EDC) and the control group was fed an isocaloric diet to 30% EDC. A pair feeding procedure was employed to control for caloric intake. Small pieces of liver randomly selected, were fixed in 2.3% -glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.2, incubated in a DAB medium and postfixed with. 2% aqueous osmium tetroxide. EM photographs were taken from sections of 3 tissue blocks from each sample (7,200X) with a Zeiss EM10-A (60 kV). With the use of a point counting method and a digital planimeter the volume density (Vv) and numerical density (Nv) were determined.


2019 ◽  
Author(s):  
Beijia Tan ◽  
Jenee Love ◽  
Leigh Harrell-Williams ◽  
Christian E. Mueller ◽  
Martin H. Jones

2018 ◽  
Vol 4 (10) ◽  
pp. 6
Author(s):  
Khemchandra Patel ◽  
Dr. Kamlesh Namdev

Age changes cause major variations in the appearance of human faces. Due to many lifestyle factors, it is difficult to precisely predict how individuals may look with advancing years or how they looked with "retreating" years. This paper is a review of age variation methods and techniques, which is useful to capture wanted fugitives, finding missing children, updating employee databases, enhance powerful visual effect in film, television, gaming field. Currently there are many different methods available for age variation. Each has their own advantages and purpose. Because of its real life applications, researchers have shown great interest in automatic facial age estimation. In this paper, different age variation methods with their prospects are reviewed. This paper highlights latest methodologies and feature extraction methods used by researchers to estimate age. Different types of classifiers used in this domain have also been discussed.


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