scholarly journals Effects of n–3 Polyunsaturated Fatty Acids and Lectins on Immunoglobulin Production by Spleen Lymphocytes of Sprague-Dawley Rats

1996 ◽  
Vol 60 (6) ◽  
pp. 1025-1027 ◽  
Author(s):  
Beong Ou Lim ◽  
Koji Yamada ◽  
Pham Hung ◽  
Takashi Watanabe ◽  
Saburo Taniguchi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Sprague Dawley ◽  
Immunoglobulin Production ◽  
Sprague Dawley Rats ◽  
Spleen Lymphocytes

scholarly journals The relationships between dietary α-linolenic: linoleic acid and rat platelet eicosapentaenoic and arachidonic acids

1996 ◽  
Vol 76 (3) ◽  
pp. 447-452 ◽  
Author(s):  
Elizabeth A. Leece ◽  
Margaret A. Allman
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Linoleic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Total Energy ◽  
Abdominal Aorta ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Arachidonic Acids

Increased dietary intake of α-linolenic acid (ALA) may be desirable to enrich tissue eicosapentaenoic acid (EPA; 20:5n-3) but competition between n-3 and n-6 fatty acids for enzymes involved in elongation and subsequent acylation will determine the relative proportions of phospholipid fatty acids. The aim of the present study was to examine the effects of altering the dietary ALA: linoleic acid (LA) ratio on rat platelet EPA and arachidonic acid (AA; 20:4n-6) concentrations. Sprague Dawley rats were fed on diets containing 30% total energy as fat with approximately 10% each of saturated, monounsaturated and polyunsaturated fatty acids with one of the following ALA:LA values; 1:7, l:4, l:1 or 1.3:1 (nine rats per group). After 4 weeks, blood was withdrawn from the abdominal aorta and platelet fatty acids analysed. The proportion of EPA was greater at the 1:1 and 1·3:1 ratios compared with the 1:7 and 1:4 (P < 0·05), and a decrease in AA was observed (P < 0·05) at the higher ratios. It was established that the platelet EPA:AA value increased (P < 0·05) as the dietary ALA:LA value increased

scholarly journals Effect of Polyunsaturated Fatty Acids on Homocysteine Metabolism through Regulating the Gene Expressions Involved in Methionine Metabolism

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Tao Huang ◽  
Xiaojie Hu ◽  
Nicholas Khan ◽  
Jing Yang ◽  
Duo Li
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Mrna Expression ◽  
Control Group ◽  
Methionine Metabolism ◽  
Regulatory Effect ◽  
Sprague Dawley ◽  
Gene Expressions ◽  
Sprague Dawley Rats ◽  
Key Genes

The objective was to investigate the regulatory effect of polyunsaturated fatty acids (PUFAs) on mRNA expression of key genes involved in homocysteine (Hcy) metabolism. Eighty male Sprague Dawley rats were randomly divided into eight groups. The oils were orally administered daily for 8 weeks. Plasma Hcy, phospholipids fatty acids, and mRNA expression were determined. Compared with the control group, plasma Hcy was significantly decreased in the 22:6n-3and conjugated linoleic acid (CLA) groups; mRNA expression ofMthfrwas significantly upregulated in the 22:6n-3, 20:5n-3, and 18:3n-3groups and downregulated in the 18:2n-6and stearolic acid (SO) groups.Mat1awas upregulated in the 22:6n-3, 20:5n-3, 18:3n-3, and CLA groups. In addition,Cbswas upregulated in the 22:6n-3, 20:5n-3, 18:3n-3and CLA groups while downregulated in 18:2n-6and SO groups. Dietary 22:6n-3and CLA decrease the plasma concentration of Hcy. mRNA expression ofMthfr, Mat1a, CbsandPemt, Gnmt, Mtrr, andBadis upregulated byn-3PUFA and downregulated byn-6PUFA. CLA upregulates mRNA expression ofMat1aandCbs.

scholarly journals Effects of repeated gestation and lactation on milkn-6 fatty acid composition in rats fed on a diet rich in 18:2n-6 or 18:3n-6

1992 ◽  
Vol 68 (2) ◽  
pp. 337-347 ◽  
Author(s):  
Yung-Sheng Huang ◽  
Peter R. Redden ◽  
David F. Horrobin ◽  
Sandra Churchill ◽  
Barbara Parker ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Milk Fat ◽  
Maternal Diet ◽  
Lactation Period ◽  
Sprague Dawley ◽  
Milk Fatty Acids ◽  
Evening Primrose ◽  
Sprague Dawley Rats ◽  
Long Chain

The present study examined the effect of repeated gestation and lactation on the levels of long-chainn-6 polyunsaturated fatty acids in rat milk fat, and examined whether such levels might be modulated by supplementing the diet of the lactating dams with either (g/kg) 50 safflower oil (SFO; containing 800 g 18:2n-6/kg), or 50 evening primrose oil (EPO; containing 720 g 18:2n-6 and 90 g 18:3n-6/kg). The milk was collected at three different times (days 1, 8 and 15) in each given lactation period from female Sprague-Dawley rats which were successively bred for four pregnancies and lactations. Results showed that dietary fat and breeding frequency had no significant effects on milk triacylglycerol content, but they modified the pattern of milk fatty acids in both triacylglycerol and phospholipid fractions. After three or four successive breedings rats fed on EPO produced milk containing less saturated but more monounsaturated and polyunsaturated fatty acids compared with those fed on SFO. During the course of lactation the levels ofn-6 metabolites, e.g. 18:3n-6, 20:3n-6 and 20:4n-6, in milk fat declined progressively. However, they were consistently higher in the EPO group than in the SFO group. These findings suggest that the levels of long-chainn-6 metabolites in the milk fat may be increased through supplementing the maternal diet with 18:3n-6.

scholarly journals The effects of polyunsaturated:saturated fatty acids ratios and peroxidisability index values of dietary fats on serum lipid profiles and hepatic enzyme activities in rats

2005 ◽  
Vol 94 (4) ◽  
pp. 526-532 ◽  
Author(s):  
Min Jeong Kang ◽  
Myoung Suk Shin ◽  
Jung Nan Park ◽  
Sang Sun Lee
Keyword(s):  
Fatty Acids ◽  
Enzyme Activities ◽  
Serum Lipid ◽  
Hdl Cholesterol ◽  
Thiobarbituric Acid ◽  
Dietary Fats ◽  
Thiobarbituric Acid Reactive Substance ◽  
Hepatic Enzyme ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Raising the dietary PUFA:saturated fatty acids (SFA) ratio has been recommended for the prevention of CVD. However, a high PUFA:SFA (P:S) ratio diet enhances oxidative stress because PUFA are highly susceptible to lipid peroxidation. Thus, we focused on the role of the dietary P:S ratio and peroxidisability index (PI) value on serum and liver tissue and investigated the effects of dietary P:S ratios (0·4, 1·0, and 4·8) with a fixed PI value (81) on serum lipid parameters and hepatic enzyme activities (experiment 1). To elucidate whether those phenomena were unique to the P:S ratio, we examined the effects of dietary PI values (36, 81, 126, and 217) with a constant P:S ratio (1·0) (experiment 2). Female Sprague–Dawley rats weighing 240–280 g were fed experimental diets for 4 weeks. When dietary PI value was maintained at 81, serum HDL-cholesterol (HDL-C) increased with increasing dietary P:S ratio. When the P:S ratio was fixed at 1·0, HDL-C was the lowest with mid–low PI (MLPI) (PI value of 81). In both experiments, serum LDL-cholesterol:HDL-C ratio kept in the range of 0–2. The hepatic superoxide dismutase, catalase, and glutathione peroxidase (GSH-Px) activities and thiobarbituric acid reactive substance (TBARS) concentrations were the highest in the lowest dietary P:S ratio group (experiment 1). GSH-Px, glutathione-S-transferase, and TBARS were the lowest in rats fed the MLPI diet (experiment 2). In conclusion, these results indicate that a P:S ratio of 1·0–1·5 and a PI value of 80–90 in the diet are within a favourable range to reduce the risk of CVD.

Site-dependent modulating effects of conjugated fatty acids from safflower oil in a rat two-stage carcinogenesis model in female Sprague–Dawley rats

2001 ◽  
Vol 168 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Naoya Kimoto ◽  
Masao Hirose ◽  
Mitsuru Futakuchi ◽  
Toshio Iwata ◽  
Masaaki Kasai ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Safflower Oil ◽  
Sprague Dawley ◽  
Two Stage ◽  
Conjugated Fatty Acids ◽  
Sprague Dawley Rats

scholarly journals Effect of Butyrylated Arrowroot Starch to the Digesta Profile and Molar Ratio SCFA

2013 ◽  
Vol 2 (2) ◽  
pp. 144
Author(s):  
Damat Damat
Keyword(s):  
Fatty Acids ◽  
Water Content ◽  
Butyric Acid ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Molar Ratio ◽  
Sprague Dawley ◽  
Randomized Design ◽  
Sprague Dawley Rats ◽  
Arrowroot Starch

The research was conducted to determine the effects of Butyrylated Arrowroot Starch (BAS) to the digesta profile and Short Chain Fatty Acids (SCFA) molar ratio. The research was conducted by using simple complete randomized design (CRD). The treatment tested was feed type, which consists of 5 levels, namely natural arrowroot starch, BAS with Degree of Substitution (DS) 0.053; 0.120; 0.187 and AIN93 natural feed. The study was conducted by using 30 Sprague Dawley rats, which were divided into 5 groups, each group consisting of 6 tails. Feeding treatments were given from dayseven until day 33. On day-34 analysis was conductedof the digesta profile and the SCFA molar ratio. Based on the analysis results, it is known that BAS feeding increased digesta weight, decreased digesta pH and increased digesta water content when compared with the AIN93 standard feed. Largest amount of digesta, lowest digesta pH, and highest water content of digesta, respectively 7.19 g, 6.53 and 91.23%, wasobtained from rats fed BAS with DS 0.187. The molar ratio of butyric acid in digesta also increased along with the increasing BAS DS.<strong><em></em></strong><em></em>

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