Fatty acid composition of yogurt drink based on mare's milk

In accordance with the local climatic conditions and folk traditions in the Republic of Bashkortostan, dairy horse breeding occupies a leading position. Mare's milk is a unique raw material for the production of a wide variety of highly nutritious, complete and easily digestible food products. Scientists recommend mare's milk as a medicinal and dietary product. The paper presents a yogurt recipe developed on the basis of a composition using mare's milk. The composition received patent No 2677219 ( Composition…, 2019 ). It is known that mare's milk fat is rich in polyunsaturated fatty acids, which are of particular physiological importance for the human body. There is a need to ration and ensure a constant intake of polyunsaturated fatty acids of the ω-3 family with food. The purpose of this work is to study the fatty acid composition of yoghurt. To obtain a fermented milk product, milk from Bashkir mares was used as raw material. The production and research of prototypes of yoghurt were carried out on the basis of the Faculty of Food Technologies of the Bashkir State Agrarian University. The determination of the fatty acid composition of the yoghurt sample took place in the accredited testing laboratory centre "Federal Research Centre for Nutrition and Biotechnology" (Moscow). The use of mare's milk allows one to change the fatty acid composition of the finished fermented milk product. Analysis of the fatty acid composition of the sample has showed that it has a high content of oleic, γ-linolenic, α-linolenic and arachidonic acids.

Effect of rearing system and sex on the composition and fatty acid profile of Andinoacara rivulatus meat from Ecuador

This study evaluated the influence of rearing system (cultured vs. wild rearing systems), and fish sex on carcass and flesh traits of A. rivulatus, raised in Ecuador. Three hundred mature specimens from A. rivulatus were captured, 150 from each origin. Slaughter yield and dress-out resulted similar for both rearing system, and average fillet yield for cultured fish was significantly higher than for wild fish, while cooking loss was significantly lower. Dress-out was significantly higher in females. Significant differences were found in wet percentage, ash, fat and protein content in both rearing systems, but only wet percentage was significantly affected by sex. Palmitic, oleic and arachidonic acids had the maximum percentage of saturated (SFA) and mono/poly unsaturated (MUFA/ PUFA) fatty acids, respectively. In cultured and wild fish, differentiations were also found in PUFA/SFA, docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA), atherogenicity (IA), and thrombogenicity (IT) indices, along with P, K, Mg, Cu and Fe. Sex and rearing system significantly influenced most of the analyzed characteristics of carcass and flesh of A. rivulatus. Fillet high yield and its proximate composition categorize A. rivulatus as a suitable food. These results provide valuable nutritional information about native species for consumers in Ecuador. Highlights: The rearing system (cultured vs. wild rearing systems), and fish sex affect the carcass and flesh traits of A. rivulatus. Fillet high yield and its proximate composition categorize A. rivulatus as a suitable food. The proximate composition of fillet from cultured A. rivulatus is more adequate than those of wild A. rivulatus. The cultured A. rivulatus contains higher n-3 and n-6 PUFA percentages and adequate n-3/n-6 ratio.

Application of Auricularia cornea as a Pork Fat Replacement in Cooked Sausage

The effect of Auricularia cornea (AC) as an alternative for pork fat on the physico-chemical properties and sensory characteristics of cooked sausage were evaluated. The results indicated that replacement of pork fat with AC led to a significant increase in the protein, ash, moisture, cooking loss, water holding capacity, springiness, and chewiness, especially isoleucine, leucine, proline, palmitic, palmitoleic, oleic, and arachidonic acids of the sausages. In contrast, AC reduced the level of fat (12.61%–87.56%) and energy (5.76%–56.40%) of the sausages. In addition, AC led to the mild lightness, yellowness, whiteness, and soft texture, while it did not affect the water activity of the sausages. From the sensory point of view, all sausages were judged acceptable, and the substitution of 75% of pork fat by AC exhibited best sensory characteristics. In a word, AC is a promising food to partially replace the pork fat in sausages.

PSVI-21 Fatty acid composition of blood lipids in lambs of different genotypes in ontogeny

The early period of postnatal ontogeny of sheep is characterized by a variety of metabolic processes that result in the formation and consolidation of a certain type of metabolism. A special role is played by lipids, which together with proteins and carbohydrates constitute the bulk of organic compounds, cells and the organism as a whole. The purpose of this research was to study the polymorphism of CAST and GH genes as well as its association with the fatty acid composition of blood plasma lipids in lambs. The content of fatty acids was determined by gas-liquid chromatography in the form of methyl esters. From the data obtained, it follows that in all studied ontogeny periods the peripheral blood of sheep with CASTNN and CASTMM genotypes had a higher concentration of fatty acids such as palmitic, stearic, linoleic, and arachidonic acids. The content is as follows: 27.58, 45.40, 3.16, 0.28% vs. 26.21, 45.31, 3.62, 0.18% at the age of 2 months; 25.07, 43.25, 6.05, 1.74% vs. 23.24, 38.76, 6.49, 1.32% at the age of 4 months; 20.64, 22.26, 14.17, 3.38% vs. 17.64, 19.91, 15.07, 2.46% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Studies of blood lipid composition of GHBBand GHAA genotype carriers showed that palmitic, stearic, linoleic, and arachidonic acids were dominant: 27.95, 27.96, 19.04% vs. 26.08, 23.88, 17.56% at the age of 2 months, 46.87, 39.87, 22.37% vs. 3.24, 6.87, 15.56% at the age of 4 months; 0.45, 1.68, 3.81% vs. 0.29, 1.29, 3.19% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Thus, this study emphasizes that there is a close relationship between the ratio of fatty acids in total blood lipids in lambs and the intensity of growth and development, ensuring and controlling the direction of biochemical processes in the growth and development of young sheep.

A plasma fatty acid profile associated to type 2 diabetes development: from the CORDIOPREV study

Purpose The prevalence of type 2 diabetes mellitus (T2DM) is increasing worldwide. For this reason, it is essential to identify biomarkers for the early detection of T2DM risk and/or for a better prognosis of T2DM. We aimed to identify a plasma fatty acid (FA) profile associated with T2DM development. Methods We included 462 coronary heart disease patients from the CORDIOPREV study without T2DM at baseline. Of these, 107 patients developed T2DM according to the American Diabetes Association (ADA) diagnosis criteria after a median follow-up of 60 months. We performed a random classification of patients in a training set, used to build a FA Score, and a Validation set, in which we tested the FA Score. Results FA selection with the highest prediction power was performed by random survival forest in the Training set, which yielded 4 out of the 24 FA: myristic, petroselinic, α-linolenic and arachidonic acids. We built a FA Score with the selected FA and observed that patients with a higher score presented a greater risk of T2DM development, with an HR of 3.15 (95% CI 2.04–3.37) in the Training set, and an HR of 2.14 (95% CI 1.50–2.84) in the Validation set, per standard deviation (SD) increase. Moreover, patients with a higher FA Score presented lower insulin sensitivity and higher hepatic insulin resistance (p < 0.05). Conclusion Our results suggest that a detrimental FA plasma profile precedes the development of T2DM in patients with coronary heart disease, and that this FA profile can, therefore, be used as a predictive biomarker. Clinical Trials.gov.Identifier NCT00924937.

Fatty acid composition and lipid quality indices of bream Abramis brama (Linnaeus, 1758) of Lake Kotokel (Western Transbaikalia)

This paper describes the study of fatty acid (FA) composition and lipid quality indices of bream Abramis brama (Linnaeus, 1758) from Lake Kotokel (Western Transbaikalia). In the muscle tissues of bream sampled in 2009 and 2019, high levels of polyunsaturated fatty acids (PUFA), including essential docosahexaenoic (DHA), eicosapentaenoic (EPA), and arachidonic acids were measured. Indicators of nutritional quality based on the fatty acid composition showed that the values of the hypocholesterolemic/hypercholesterolemic (HH) ratio indices were sufficiently high. The atherogenicity (AI) and thrombogenicity (TI) indices, which are indicators for the nutritional value, were less than 1 in the studied fish. In terms of flesh-lipid quality (FLQ), bream had the highest proportion of total EPA + DHA. According to the obtained data for the composition of fatty acids in the muscle tissue of the studied fish from Lake Kotokel, the anthropogenic load on Lake Kotokel has not yet had a statistically significant effect on the fish muscle quality.

The Comparison of Fatty Acid Composition and Lipid Quality Indices of Roach, Perch, and Pike of Lake Gusinoe (Western Transbaikalia)

This paper describes the study of the fatty acid (FA) composition of three fish species (roach, perch, and pike) from Lake Gusinoe (western Transbaikalia). Using principal component analysis, the fatty acid composition of the studied fish species was shown to be species specific. The muscle tissue of roach, perch, and pike was found to contain high levels of polyunsaturated fatty acids (PUFA), including essential docosahexaenoic (DHA), eicosapentaenoic (EPA), and arachidonic acids. Indicators of nutritional quality based on the fatty acid composition showed that the values of the hypocholesterolemic/hypercholesterolemic (HH) ratio indices were sufficiently high. The atherogenicity (AI) and thrombogenicity (TI) indices, which are indicators for the nutritional value, were less than 1 in the studied fish. In terms of flesh lipid quality (FLQ), pike and perch had the highest proportion of total EPA + DHA. According to the obtained data for the composition of fatty acids in the muscle tissue of the studied fish from Lake Gusinoe, the anthropogenic load exerted on Lake Gusinoe has not yet statistically significantly affected the fish muscle quality.

Effects of Replacing Yellow Corn with Olive Cake Meal on Growth Performance, Plasma Lipid Profile, and Muscle Fatty Acid Content in Broilers

The current study focused exclusively on evaluating the effects of replacing corn with olive cake meal (OCM) in the diet of broilers on their growth performance, abdominal fat, selected plasma parameters, and muscle fatty acid (FA) content. A total of 480 one-day-old male broiler chickens (Ross 308) were divided into four treatment groups with 12 replicates/treatment. The control group was fed the base diet, whereas the second to fourth groups were fed diets of corn with 5%, 10%, and 20% contents of OCM, respectively. Broilers fed with the 5% and 10% OCM diets showed better body weight (p = 0.04) and feed conversion ratio than the 20% OCM group (p < 0.048). Both nitrogen retention and ether extract digestibility were not improved by replaced corn with OCM. Replacing corn with OCM led to a decreased abdominal fat percentage (p = 0.023) compared with the control group. Birds in the OCM groups showed the lowest total cholesterol values (p = 0.038). The breast muscle (musculus pectoralis superficialis) content of oleic and linoleic, linolenic, and arachidonic acids was significantly high in birds fed with OCM diets. However, their palmitic acid level was significantly decreased. Vitamin E was increased by increasing the OCM level. Thus, we concluded that replacing corn with OCM, especially at a 10% level, is more effective than other replacement levels in improving growth performance, plasma lipid profile, and muscle FA content, as well as in causing a reduction in abdominal fat in broilers.

High plasma concentration of non-esterified polyunsaturated fatty acids is a specific feature of severe COVID-19 pneumonia

COVID-19 pneumonia has specific features and outcomes that suggests a unique immunopathogenesis. Severe forms of COVID-19 appear to be more frequent in obese patients, but an association with metabolic disorders is not established. Here, we focused on lipoprotein metabolism in patients hospitalized for severe pneumonia, depending on COVID-19 status. Thirty-four non-COVID-19 and 27 COVID-19 patients with severe pneumonia were enrolled. Most of them required intensive care. Plasma lipid levels, lipoprotein metabolism, and clinical and biological (including plasma cytokines) features were assessed. Despite similar initial metabolic comorbidities and respiratory severity, COVID-19 patients displayed a lower acute phase response but higher plasmatic concentrations of non-esterified fatty acids (NEFAs). NEFA profiling was characterised by higher level of polyunsaturated NEFAs (mainly linoleic and arachidonic acids) in COVID-19 patients. Multivariable analysis showed that among severe pneumonia, COVID-19-associated pneumonia was associated with higher NEFAs, lower apolipoprotein E and lower high-density lipoprotein cholesterol concentrations, independently of body mass index, sequential organ failure (SOFA) score, and C-reactive protein levels. NEFAs and PUFAs concentrations were negatively correlated with the number of ventilator-free days. Among hospitalized patients with severe pneumonia, COVID-19 is independently associated with higher NEFAs (mainly linoleic and arachidonic acids) and lower apolipoprotein E and HDL concentrations. These features might act as mediators in COVID-19 pathogenesis and emerge as new therapeutic targets. Further investigations are required to define the role of NEFAs in the pathogenesis and the dysregulated immune response associated with COVID-19.Trial registration: NCT04435223.