scholarly journals Studies on pharmacological activation of human serum IgG by chemical modification and active subfragments. I. Induction of anti-ulcerogenic activity by chemical cleavage of interchain disulfide bonds in human IgG and properties of active subfragments.

1983 ◽  
Vol 6 (6) ◽  
pp. 397-407
Author(s):  
TSUTOMU MIMURA ◽  
TAICHIRO TERADA ◽  
MASAKAZU IWAI ◽  
ISAO KOHDA ◽  
SETSUYO TAKE ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Human Serum ◽  
Disulfide Bonds ◽  
Human Igg ◽  
Chemical Cleavage ◽  
Serum Igg ◽  
Ulcerogenic Activity

Quantitation of Serum IgG by Solid-Phase Radioimmunoassay

1974 ◽  
Vol 20 (3) ◽  
pp. 376-382 ◽  
Author(s):  
Leo P Cawley ◽  
William L Goodwin ◽  
Marilyn Moeder ◽  
Paulette Dibbern
Keyword(s):  
Human Serum ◽  
Sensitivity And Specificity ◽  
Solid Phase ◽  
Diffusion Method ◽  
Human Igg ◽  
Serum Igg ◽  
Hospital Patients ◽  
Solid Phase Radioimmunoassay ◽  
Set Up ◽  
Gel Diffusion

Abstract Human serum immunoglobulins are most frequently measured by single gel diffusion. Radioimmunoassay by use of antibodies bonded to particles is faster and more sensitive, precise, accurate, and specific. We adopted a solid-phase radioimmunoassay (SPRIA) technique, developed in our laboratory, to measure serum IgG, and compared the results with those of a gel-diffusion method commonly used to measure serum IgG. The SPRIA technique is based on competition set up between antibodies to human IgG chemically bonded to cellulose particles and a purified serum IgG labeled with 125I. The solid phase permits prompt separation of the bound from free labeled reagent. We studied precision, accuracy, sensitivity, and specificity, as compared to gel diffusion; values for the two methods agreed well for 57 sera from hospital patients. The SPRIA procedure is more precise, and can be completed in 3 h, as compared to the 16-24 h normally required for gel diffusion procedures. The possible automation of SPRIA is discussed.

Structural Analysis of an N-Glycan with “β1−4 Bisecting Branch” from Human Serum IgG by Negative-Ion MSnSpectral Matching and Exoglycosidase Digestion

2005 ◽  
Vol 77 (18) ◽  
pp. 6062-6068 ◽  
Author(s):  
Yasuhiro Takegawa ◽  
Kisaburo Deguchi ◽  
Hiroaki Nakagawa ◽  
Shin-Ichiro Nishimura
Keyword(s):  
Structural Analysis ◽  
Human Serum ◽  
Negative Ion ◽  
Serum Igg
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