Structural Analysis of an N-Glycan with “β1−4 Bisecting Branch” from Human Serum IgG by Negative-Ion MSnSpectral Matching and Exoglycosidase Digestion

2005 ◽  
Vol 77 (18) ◽  
pp. 6062-6068 ◽  
Author(s):  
Yasuhiro Takegawa ◽  
Kisaburo Deguchi ◽  
Hiroaki Nakagawa ◽  
Shin-Ichiro Nishimura
Keyword(s):  
Structural Analysis ◽  
Human Serum ◽  
Negative Ion ◽  
Serum Igg

Development of a novel multiplex electrochemiluminescent-based immunoassay for quantification of human serum IgG against 10 Staphylococcus aureus toxins

2016 ◽  
Vol 430 ◽  
pp. 33-42 ◽  
Author(s):  
Rajan P. Adhikari ◽  
Christian Haudenschild ◽  
Patricia M. Sterba ◽  
Sara Sahandi ◽  
Sven Enterlein ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Human Serum ◽  
Serum Igg

Absolute quantitation of high abundant Fc-glycopeptides from human serum IgG-1

2020 ◽  
Vol 1102 ◽  
pp. 130-139 ◽  
Author(s):  
Cuiyan Cao ◽  
Long Yu ◽  
Dongmei Fu ◽  
Jingli Yuan ◽  
Xinmiao Liang
Keyword(s):  
Human Serum ◽  
Absolute Quantitation ◽  
Serum Igg

Structural Analysis of Human Serum Albumin Complexes with Cationic Lipids

2009 ◽  
Vol 113 (6) ◽  
pp. 1777-1784 ◽  
Author(s):  
David Charbonneau ◽  
Marc Beauregard ◽  
Heidar-Ali Tajmir-Riahi
Keyword(s):  
Structural Analysis ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Cationic Lipids

Structural Analysis of N-Glycans by the Glycan-Labeling Method Using 3-Aminoquinoline-Based Liquid Matrix in Negative-Ion MALDI-MS

2012 ◽  
Vol 84 (21) ◽  
pp. 9453-9461 ◽  
Author(s):  
Takashi Nishikaze ◽  
Kaoru Kaneshiro ◽  
Shin-ichirou Kawabata ◽  
Koichi Tanaka
Keyword(s):  
Structural Analysis ◽  
Maldi Ms ◽  
Negative Ion ◽  
Liquid Matrix ◽  
Labeling Method

Negative Ion Mass Spectra of Some Pesticidal Compounds

1971 ◽  
Vol 54 (6) ◽  
pp. 1340-1348 ◽  
Author(s):  
Peter C Rankin
Keyword(s):  
Organic Chemistry ◽  
Structural Analysis ◽  
Characteristic Feature ◽  
Mass Spectra ◽  
Organic Molecules ◽  
Negative Ions ◽  
Negative Ion ◽  
Carbamate Pesticides ◽  
Complex Organic

Abstract A study of the negative ion mass spectra of a number of complex organic molecules (pesticides) was undertaken to determine what information this technique would yield for structural analysis. The simplicity of the negative ion mass spectra was a characteristic feature and the most abundant negative ions in the spectra were assumed to be similar to the stable carbanions postulated in classical organic chemistry. The simplicity of the mass spectra suggested a possible application of the technique to the identification of carbamate pesticides.

Method Development and Validation for Ultra-High-Pressure LC/MS/MS Determination of Hop Prenylflavonoids in Human Serum

2012 ◽  
Vol 95 (6) ◽  
pp. 1744-1749 ◽  
Author(s):  
Yang Yuan ◽  
Xi Qiu ◽  
Dejan Nikolic ◽  
Jeffrey H Dahl ◽  
Richard B van Breemen
Keyword(s):  
High Pressure ◽  
Human Serum ◽  
Method Development ◽  
Hot Flashes ◽  
Cancer Chemoprevention ◽  
Gradient Elution ◽  
Negative Ion ◽  
Selected Reaction Monitoring ◽  
Ultra High Pressure

Abstract Hops (Humulus lupulus L.) are used in the brewing of beer, and hop extracts containing prenylated compounds, such as xanthohumol (XN) and 8-prenylnaringenin (8-PN), are under investigation as dietary supplements for cancer chemoprevention and the management of hot flashes in menopausal women. To facilitate clinical studies of hop safety and efficacy, a selective, sensitive, and fast ultra-high-pressure LC (UHPLC) tandem MS method was developed and validated for the simultaneous determination of the hop prenylflavonoids XN, isoxanthohumol (IX), 6-prenylnaringenin (6-PN), and 8-PN in human serum. The analytical method requires 300 μL of human serum, which is processed using liquid–liquid extraction. UHPLC separation was carried out in 2.5 min with gradient elution using an RP C18 column containing 1.6 μm particle size packing material. Prenylflavonoids were measured using negative ion electrospray MS with collision-induced dissociation and selected reaction monitoring. The method was validated and showed good accuracy and precision with an LOQ of 0.50 ng/mL for XN (1.4 nM) and 1.0 ng/mL for 6-PN, 8-PN (2.94 nM), and IX (2.82 nM) in serum.

Sign in / Sign up

Export Citation Format

Share Document