Covalent Immobilization of Oligonucleotides on Modified Glass/Silicon Surfaces for Solid-phase DNA Hybridization and Amplification

1998 ◽  
Vol 27 (3) ◽  
pp. 257-258 ◽  
Author(s):  
Mengsu Yang ◽  
Richard Y. C. Kong ◽  
Najam Kazmi ◽  
Andrew K. C. Leung
Keyword(s):  
Dna Hybridization ◽  
Solid Phase ◽  
Covalent Immobilization ◽  
Silicon Surfaces

A Simple Method for Covalent Immobilization of Proteins on Porous Silicon Surfaces

2005 ◽  
Vol 34 (2) ◽  
pp. 226-227 ◽  
Author(s):  
Bing Xia ◽  
Jun Li ◽  
Shou-Jun Xiao ◽  
Dong-Jie Guo ◽  
Jing Wang ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Covalent Immobilization ◽  
Silicon Surfaces ◽  
Simple Method

Solid-Phase Chemical Amination of a Lipase from Bacillus thermocatenulatus To Improve Its Stabilization via Covalent Immobilization on Highly Activated Glyoxyl-Agarose

2008 ◽  
Vol 9 (9) ◽  
pp. 2553-2561 ◽  
Author(s):  
Gloria Fernandez-Lorente ◽  
Cesar A. Godoy ◽  
Adriano A. Mendes ◽  
Fernando Lopez-Gallego ◽  
Valeria Grazu ◽  
...  
Keyword(s):  
Solid Phase ◽  
Covalent Immobilization ◽  
Phase Chemical

Covalent Immobilization of a TiW5 Polyoxometalate on Derivatized Silicon Surfaces

2005 ◽  
Vol 44 (8) ◽  
pp. 1254-1257 ◽  
Author(s):  
R. John Errington ◽  
Sagar S. Petkar ◽  
Benjamin R. Horrocks ◽  
Andrew Houlton ◽  
Lars H. Lie ◽  
...  
Keyword(s):  
Covalent Immobilization ◽  
Silicon Surfaces

scholarly journals Electrochemical Detection Platform Based on RGO Functionalized with Diazonium Salt for DNA Hybridization

Biosensors ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 39
Author(s):  
Elena A. Chiticaru ◽  
Luisa Pilan ◽  
Mariana Ioniţă
Keyword(s):  
Charge Transfer ◽  
Dna Hybridization ◽  
Electrochemical Impedance ◽  
Diazonium Salt ◽  
Modified Electrodes ◽  
Covalent Immobilization ◽  
Fabrication Process ◽  
Label Free ◽  
Modified Dna ◽  
Electron Transfer Properties

In this paper, we propose an improved electrochemical platform based on graphene for the detection of DNA hybridization. Commercial screen-printed carbon electrodes (SPCEs) were used for this purpose due to their ease of functionalization and miniaturization opportunities. SPCEs were modified with reduced graphene oxide (RGO), offering a suitable surface for further functionalization. Therefore, aryl-carboxyl groups were integrated onto RGO-modified electrodes by electrochemical reduction of the corresponding diazonium salt to provide enough reaction sites for the covalent immobilization of amino-modified DNA probes. Our final goal was to determine the optimum conditions needed to fabricate a simple, label-free RGO-based electrochemical platform to detect the hybridization between two complementary single-stranded DNA molecules. Each modification step in the fabrication process was monitored by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using [Fe(CN)6]3−/4− as a redox reporter. Although, the diazonium electrografted layer displayed the expected blocking effect of the charge transfer, the next steps in the modification procedure resulted in enhanced electron transfer properties of the electrode interface. We suggest that the improvement in the charge transfer after the DNA hybridization process could be exploited as a prospective sensing feature. The morphological and structural characterization of the modified electrodes performed by scanning electron microscopy (SEM) and Raman spectroscopy, respectively, were used to validate different modification steps in the platform fabrication process.

scholarly journals Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study

2010 ◽  
Vol 38 (20) ◽  
pp. 7343-7351 ◽  
Author(s):  
Tomáš Špringer ◽  
Hana Šípová ◽  
Hana Vaisocherová ◽  
Josef Štěpánek ◽  
Jiří Homola
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Dna Hybridization ◽  
Solid Phase ◽  
Divalent Cations ◽  
Shielding Effect ◽  
Surface Plasmon Resonance Biosensor

Rotating Rod Renewable Microcolumns for Automated, Solid-Phase DNA Hybridization Studies

2000 ◽  
Vol 72 (17) ◽  
pp. 4135-4141 ◽  
Author(s):  
Cynthia J. Bruckner-Lea ◽  
Mark S. Stottlemyre ◽  
David A. Holman ◽  
Jay W. Grate ◽  
Fred J. Brockman ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Solid Phase

Covalent immobilization and solid-phase refolding of enterokinase for fusion protein cleavage

2005 ◽  
Vol 40 (5) ◽  
pp. 1755-1762 ◽  
Author(s):  
Chang Woo Suh ◽  
Sin Hye Park ◽  
Seung Gook Park ◽  
Eun Kyu Lee
Keyword(s):  
Fusion Protein ◽  
Solid Phase ◽  
Covalent Immobilization ◽  
Protein Cleavage ◽  
Fusion Protein Cleavage
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