Covalent immobilization and solid-phase refolding of enterokinase for fusion protein cleavage

2005 ◽  
Vol 40 (5) ◽  
pp. 1755-1762 ◽  
Author(s):  
Chang Woo Suh ◽  
Sin Hye Park ◽  
Seung Gook Park ◽  
Eun Kyu Lee
Keyword(s):  
Fusion Protein ◽  
Solid Phase ◽  
Covalent Immobilization ◽  
Protein Cleavage ◽  
Fusion Protein Cleavage

scholarly journals Phylogenetic Relationships among Highly Virulent Newcastle Disease Virus Isolates Obtained from Exotic Birds and Poultry from 1989 to 1996

1998 ◽  
Vol 36 (4) ◽  
pp. 1141-1145 ◽  
Author(s):  
Bruce S. Seal ◽  
Daniel J. King ◽  
Devin P. Locke ◽  
Dennis A. Senne ◽  
Mark W. Jackwood
Keyword(s):  
United States ◽  
Fusion Protein ◽  
Newcastle Disease ◽  
Cleavage Site ◽  
The United States ◽  
Protein Cleavage ◽  
Exotic Birds ◽  
Fusion Protein Cleavage Site ◽  
Highly Virulent ◽  
Fusion Protein Cleavage

Newcastle disease virus {NDV (avian paramyxovirus type 1 [APMV1])} isolates were recovered from imported exotic birds confiscated following importation into the United States, from waterbirds in the United States, and from poultry. The exotic birds probably originated from Central and South America, Asia, and Africa. The NDV isolates were initially characterized as highly virulent because of a short mean death time in embryonated chicken eggs. The isolates were typed as neurotropic or viscerotropic velogenic by intracloacal inoculation of adult chickens. Intracerebral pathogenicity index values for the virulent NDV isolates ranged from 1.54 to 1.90, compared to a possible maximum value of 2.0. These isolates had a dibasic amino acid motif in the fusion protein cleavage site sequence required for host systemic replication. Sequence differences were detected surrounding the fusion protein cleavage site and the matrix protein nuclear localization signal, indicating evolution of highly virulent NDV. Phylogenetically, these isolates were categorized with other highly virulent NDV strains that caused outbreaks in southern California poultry during 1972 and in cormorants in the north central United States and southern Canada during 1990 and 1992. These isolates are related to NDV that may have the APMV1 strain chicken/Australia/AV/32 or a related virus as a possible progenitor. Recent virulent NDV isolates and those recovered during disease outbreaks since the 1970s are phylogenetically distinct from current vaccine viruses and standard challenge strains.

scholarly journals Evaluation of fusion protein cleavage site sequences of Newcastle disease virus in genotype matched vaccines

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173965 ◽  
Author(s):  
Shin-Hee Kim ◽  
Zongyan Chen ◽  
Asuka Yoshida ◽  
Anandan Paldurai ◽  
Sa Xiao ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Cleavage Site ◽  
Protein Cleavage ◽  
Fusion Protein Cleavage Site ◽  
Fusion Protein Cleavage

scholarly journals Coupling of MBP fusion protein cleavage with sparse matrix crystallization screens to overcome problematic protein solubility

BioTechniques ◽  
2005 ◽  
Vol 39 (4) ◽  
pp. 476-480 ◽  
Author(s):  
Franz Gruswitz ◽  
Mary Frishman ◽  
Barry M. Goldstein ◽  
Joseph E. Wedekind
Keyword(s):  
Fusion Protein ◽  
Sparse Matrix ◽  
Protein Solubility ◽  
Protein Cleavage ◽  
Fusion Protein Cleavage
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