Rotating Rod Renewable Microcolumns for Automated, Solid-Phase DNA Hybridization Studies

2000 ◽  
Vol 72 (17) ◽  
pp. 4135-4141 ◽  
Author(s):  
Cynthia J. Bruckner-Lea ◽  
Mark S. Stottlemyre ◽  
David A. Holman ◽  
Jay W. Grate ◽  
Fred J. Brockman ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Solid Phase

scholarly journals Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study

2010 ◽  
Vol 38 (20) ◽  
pp. 7343-7351 ◽  
Author(s):  
Tomáš Špringer ◽  
Hana Šípová ◽  
Hana Vaisocherová ◽  
Josef Štěpánek ◽  
Jiří Homola
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Dna Hybridization ◽  
Solid Phase ◽  
Divalent Cations ◽  
Shielding Effect ◽  
Surface Plasmon Resonance Biosensor

Spacer length, label moiety interchange and probe pair orientation in a homogeneous solid-phase hybridization assay utilizing lanthanide chelate complementation

2014 ◽  
Vol 6 (14) ◽  
pp. 5360-5368 ◽  
Author(s):  
Susanne Lahdenperä ◽  
Julius Manninen ◽  
Laura Joki ◽  
Ulla Karhunen ◽  
Tero Soukka
Keyword(s):  
Dna Hybridization ◽  
Solid Phase ◽  
Spatial Multiplexing ◽  
Probe Pair ◽  
Hybridization Assay ◽  
Spacer Length ◽  
Lanthanide Chelate ◽  
Solid Phase Assay

We have studied parameters affecting DNA hybridization and lanthanide chelate complementation based signal formation in a separation-free solid-phase assay suitable for spatial multiplexing.

Use of DNA immobilized on plastic and agarose supports to detect DNA by sandwich hybridization.

1985 ◽  
Vol 31 (9) ◽  
pp. 1438-1443 ◽  
Author(s):  
R Polsky-Cynkin ◽  
G H Parsons ◽  
L Allerdt ◽  
G Landes ◽  
G Davis ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Clinical Laboratory ◽  
Solid Phase ◽  
Single Layer ◽  
Base Sequence ◽  
Solid Supports ◽  
Sandwich Hybridization ◽  
Laboratory Procedures ◽  
Hybridization Protocol ◽  
Routine Clinical Laboratory

Abstract Cloned Salmonella DNA, which has been immobilized irreversibly on plastic and agarose solid supports, can form hybrids in both single-layer and "sandwich" hybridization protocols. In single-layer hybridization, 3 micrograms of immobilized DNA bound at least 30 fmol of a specific 800-base DNA sequence (equivalent to 8.5 ng, or the amount of that sequence present in 4 X 10(10) organisms). In a 4-h sandwich hybridization protocol, as little as 14 amol (equivalent to 8 pg, or the amount of that sequence present in 1 X 10(7) organisms) of a 1600-base sequence of DNA could be detected. The methods described should be applicable to use with any set of probes--not just from Salmonella--that fulfill the criteria specified. The ability to perform DNA hybridizations on solid-phase matrices such as those used for immunoassay should bring DNA hybridization into the realm of routine clinical laboratory procedures.

Self-heating tilted fiber Bragg grating device for melt curve analysis of solid-phase DNA hybridization and thermal cycling

2019 ◽  
Vol 411 (26) ◽  
pp. 6813-6823 ◽  
Author(s):  
Jason Koppert ◽  
Hubert Jean-Ruel ◽  
Devin O’Neill ◽  
Chris Harder ◽  
William Willmore ◽  
...  
Keyword(s):  
Thermal Cycling ◽  
Fiber Bragg Grating ◽  
Dna Hybridization ◽  
Solid Phase ◽  
Curve Analysis ◽  
Bragg Grating ◽  
Melt Curve Analysis ◽  
Self Heating

Covalent Immobilization of Oligonucleotides on Modified Glass/Silicon Surfaces for Solid-phase DNA Hybridization and Amplification

1998 ◽  
Vol 27 (3) ◽  
pp. 257-258 ◽  
Author(s):  
Mengsu Yang ◽  
Richard Y. C. Kong ◽  
Najam Kazmi ◽  
Andrew K. C. Leung
Keyword(s):  
Dna Hybridization ◽  
Solid Phase ◽  
Covalent Immobilization ◽  
Silicon Surfaces

Solid-Phase PNA Synthesis and In Situ Scintillation Proximity Assay for the Detection of PNA−DNA Hybridization

2006 ◽  
Vol 8 (1) ◽  
pp. 1-3
Author(s):  
Mark C. McCairn ◽  
Marcus D. Hughes ◽  
Anna V. Hine ◽  
Andrew J. Sutherland
Keyword(s):  
Dna Hybridization ◽  
Solid Phase ◽  
Scintillation Proximity Assay ◽  
Pna Synthesis

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

Dynamic studies of silicide-mediated crystallization of amorphous silicon

1992 ◽  
Vol 50 (2) ◽  
pp. 1352-1353
Author(s):  
C. Hayzelden ◽  
J. L. Batstone
Keyword(s):  
Ion Implantation ◽  
Solid Phase ◽  
Metallic Phase ◽  
Single Crystal Substrate ◽  
Free Electrons ◽  
Melt Temperature ◽  
Transmission Electron ◽  
Crystal Substrate ◽  
High Resolution Tem

Epitaxial reordering of amorphous Si(a-Si) on an underlying single-crystal substrate occurs well below the melt temperature by the process of solid phase epitaxial growth (SPEG). Growth of crystalline Si(c-Si) is known to be enhanced by the presence of small amounts of a metallic phase, presumably due to an interaction of the free electrons of the metal with the covalent Si bonds near the growing interface. Ion implantation of Ni was shown to lower the crystallization temperature of an a-Si thin film by approximately 200°C. Using in situ transmission electron microscopy (TEM), precipitates of NiSi2 formed within the a-Si film during annealing, were observed to migrate, leaving a trail of epitaxial c-Si. High resolution TEM revealed an epitaxial NiSi2/Si(l11) interface which was Type A. We discuss here the enhanced nucleation of c-Si and subsequent silicide-mediated SPEG of Ni-implanted a-Si.Thin films of a-Si, 950 Å thick, were deposited onto Si(100) wafers capped with 1000Å of a-SiO2. Ion implantation produced sharply peaked Ni concentrations of 4×l020 and 2×l021 ions cm−3, in the center of the films.

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