Solid-Phase Covalent Immobilization of Upconverting Nanoparticles for Biosensing by Luminescence Resonance Energy Transfer

2014 ◽  
Vol 6 (16) ◽  
pp. 14061-14068 ◽  
Author(s):  
Samer Doughan ◽  
Yi Han ◽  
Uvaraj Uddayasankar ◽  
Ulrich J. Krull
Keyword(s):  
Energy Transfer ◽  
Solid Phase ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Covalent Immobilization

Inorganic Nanoparticles as Donors in Resonance Energy Transfer for Solid-Phase Bioassays and Biosensors

Langmuir ◽  
2017 ◽  
Vol 33 (45) ◽  
pp. 12839-12858 ◽  
Author(s):  
Yi Han ◽  
M. Omair Noor ◽  
Abootaleb Sedighi ◽  
Uvaraj Uddayasankar ◽  
Samer Doughan ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Solid Phase ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Inorganic Nanoparticles

scholarly journals A New Fluorogenic Substrate for Granzyme B Based on Fluorescence Resonance Energy Transfer

2020 ◽  
Vol 3 (1) ◽  
pp. 6
Author(s):  
Cátia D. F. Martins ◽  
M. Manuela M. Raposo ◽  
Susana P. G. Costa
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Solid Phase ◽  
Granzyme B ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Preparative Hplc ◽  
Fluorogenic Substrate ◽  
Acceptor Pair ◽  
Fluorescence Resonance

The synthesis and characterization of a new fluorogenic substrate for granzyme B (GzmB) is reported. The substrate design was based on the fluorescence resonance energy transfer (FRET) principle using 5-(2′-aminoethyl)aminonaphthalene sulfonic acid (Edans) and 4-[[4′-(N,N-dimethylamino)phenyl]diazenyl]benzoic acid (Dabcyl) as a donor–acceptor pair, linked to a specific sequence for GzmB (AAD), with an additional amino acid as the anchoring point (K). The tetrapeptide was synthesized by microwave-assisted solid-phase peptide synthesis (MW-SPPS) and coupled to Dabcyl and Edans at its N- and C-termini, respectively. The obtained probe was purified by semi-preparative HPLC and characterized by NMR, UV/Vis absorption and fluorescence spectroscopy and mass spectrometry.

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