scholarly journals Inferences on RNA synthesis rates during mitosis from flow cytofluorimetric RNA histograms.

1978 ◽  
Vol 26 (9) ◽  
pp. 691-695 ◽  
Author(s):  
J V Watson ◽  
S H Chambers
Keyword(s):  
Acridine Orange ◽  
Rna Synthesis ◽  
Acridine Orange Staining ◽  
Rna Content ◽  
Mitotic Cells ◽  
Rna Levels

Flow cytofluorimetric techniques with acridine orange staining have been used to study RNA levels of cells during and immediately after mitosis. It can be inferred from the histogram data that, of the mitotic cells, those in telophase have the highest RNA content, and that the rate of synthesis increases rapidly between anaphase and telophase. These inferences correlate with results obtained from parallel labeled precursor studies.

scholarly journals Total cellular RNA content: correlation between flow cytometry and ultraviolet spectroscopy.

1980 ◽  
Vol 28 (6) ◽  
pp. 493-498 ◽  
Author(s):  
K D Bauer ◽  
L A Dethlefsen
Keyword(s):  
Flow Cytometry ◽  
Acridine Orange ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ultraviolet Spectroscopy ◽  
Acridine Orange Staining ◽  
Red Fluorescence ◽  
Rna Content ◽  
Hela S3 ◽  
Hela S3 Cells

Total RNA content in Chinese hamster ovary and HeLa-S3 cells determined by ultraviolet spectroscopy is compared with the red fluorescence distribution of acridine orange-stained cells observed by flow cytometry. A correlation coefficient of 0.93 is obtained when these methods of estimating RNA content are compared after various RNAse treatments. These data suggest that acridine orange staining effectively quantitates total cellular RNA content when analyzed by flow cytometry, although DNA is also shown to contribute a low but significant background of red fluorescence.

scholarly journals Cytophotometric analyses of myocardial RNA in rats exposed to altitude hypoxia.

1978 ◽  
Vol 26 (6) ◽  
pp. 459-467 ◽  
Author(s):  
A J Stere ◽  
N W Brister ◽  
A Anthony
Keyword(s):  
Coefficient Of Variation ◽  
Rna Synthesis ◽  
Prolonged Exposure ◽  
Progressive Increase ◽  
High Variability ◽  
Vascular Responses ◽  
Hypoxia Exposure ◽  
Azure B ◽  
Exposure Interval ◽  
Rna Levels

Analytical cytophotometry of azure B-stained heart sections was employed to investigate the pattern of myocardial ribonucleic acid (RNA) alterations in rats exposed to acute (1-2 days) and prolonged periods (1-8 weeks) of hypoxia exposure (380 torr). Data support the existence of a slight transient drop in myocardial RNA on day one of exposure followed by a restoration to levels slightly elevated over controls during a 1- to 8-week exposure interval. Because of the high variability in RNA levels among myocytes (coefficient of variation, ca 40%), a shift in the proportion of low and high RNA containing segments of the myocyte population proved to be a more sensitive indicator of suppression or augmentation of RNA synthesis than the use of average RNA levels of the cell population analyzed. Microscopic analyses revealed the presence of compensatory vascular responses which could be effective in ameliorating the extent of tissue hypoxemia, i.e., capillary vasodilation on day 1 with a progressive increase in vascularization with prolonged exposure.

STUDIES ON THE BIOLOGICAL PROPERTIES AND CLASSIFICATION OF SV4 VIRUS

1965 ◽  
Vol 11 (2) ◽  
pp. 325-335 ◽  
Author(s):  
S. A. Sattar ◽  
K. R. Rozee
Keyword(s):  
Electron Microscope ◽  
Rhesus Monkey ◽  
Red Blood Cells ◽  
Acridine Orange ◽  
Blood Cells ◽  
Magnesium Chloride ◽  
Fluorescent Microscopy ◽  
Biological Properties ◽  
Acridine Orange Staining

Cytopathic changes in LLC-MK2 cells infected with SV4 virus, observed with the electron microscope and using acridine orange staining and fluorescent microscopy, have been shown to be similar to that caused by picornaviruses and members of the Columbia-SK virus group. The virus was found to be stabilized against heat in the presence of molar magnesium chloride, and to be stable at pH 3.5. The virus was non-pathogenic for suckling mice, failed to agglutinate sheep and human "O" red blood cells, but agglutinated rhesus monkey erythrocytes at 4 °C. On the basis of these properties and those already known, it was suggested that SV4 virus be placed in the group Enteroviruses of lower animals.

scholarly journals CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

1962 ◽  
Vol 15 (3) ◽  
pp. 535-540 ◽  
Author(s):  
M. Rabinovitch ◽  
W. Plaut
Keyword(s):  
Nucleic Acid ◽  
Dna Synthesis ◽  
Acridine Orange ◽  
Particle Number ◽  
Amoeba Proteus ◽  
Acridine Orange Staining ◽  
Particle Population ◽  
Cell Age ◽  
Cytoplasmic Dna ◽  
Number Of Particles

Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.

The fluorescent staining of a mycobacteriophage (C2) nucleic acid

1971 ◽  
Vol 17 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Jose Menezes
Keyword(s):  
Nucleic Acid ◽  
Acridine Orange ◽  
Staining Technique ◽  
Fluorescent Staining ◽  
Green Fluorescence ◽  
Acridine Orange Staining ◽  
Fluorescence Characteristic ◽  
Phage Dna ◽  
Acid Dnase

By using the acridine orange staining technique a green fluorescence, characteristic of double-stranded nucleic acid, can be observed with purified preparations of mycobacteriophage C2 and its extracted nucleic acid. DNAse-treated samples do not show this fluorescence, which leads to the conclusion that this fluorescence is associated with phage DNA. Examination of preparations of phage grown in the presence of acridine orange supported these results.

An improved acridine orange staining of DNA/RNA

2019 ◽  
Vol 121 (4) ◽  
pp. 450-454 ◽  
Author(s):  
Danilo M. Damas-Souza ◽  
Rony Nunes ◽  
Hernandes F. Carvalho
Keyword(s):  
Acridine Orange ◽  
Acridine Orange Staining
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