scholarly journals Total cellular RNA content: correlation between flow cytometry and ultraviolet spectroscopy.

1980 ◽  
Vol 28 (6) ◽  
pp. 493-498 ◽  
Author(s):  
K D Bauer ◽  
L A Dethlefsen
Keyword(s):  
Flow Cytometry ◽  
Acridine Orange ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ultraviolet Spectroscopy ◽  
Acridine Orange Staining ◽  
Red Fluorescence ◽  
Rna Content ◽  
Hela S3 ◽  
Hela S3 Cells

Total RNA content in Chinese hamster ovary and HeLa-S3 cells determined by ultraviolet spectroscopy is compared with the red fluorescence distribution of acridine orange-stained cells observed by flow cytometry. A correlation coefficient of 0.93 is obtained when these methods of estimating RNA content are compared after various RNAse treatments. These data suggest that acridine orange staining effectively quantitates total cellular RNA content when analyzed by flow cytometry, although DNA is also shown to contribute a low but significant background of red fluorescence.

Efficient enrichment of high-producing recombinant Chinese hamster ovary cells for monoclonal antibody by flow cytometry

2015 ◽  
Vol 120 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Takeshi Okumura ◽  
Kenji Masuda ◽  
Kazuhiko Watanabe ◽  
Kenji Miyadai ◽  
Koichi Nonaka ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

scholarly journals Inferences on RNA synthesis rates during mitosis from flow cytofluorimetric RNA histograms.

1978 ◽  
Vol 26 (9) ◽  
pp. 691-695 ◽  
Author(s):  
J V Watson ◽  
S H Chambers
Keyword(s):  
Acridine Orange ◽  
Rna Synthesis ◽  
Acridine Orange Staining ◽  
Rna Content ◽  
Mitotic Cells ◽  
Rna Levels

Flow cytofluorimetric techniques with acridine orange staining have been used to study RNA levels of cells during and immediately after mitosis. It can be inferred from the histogram data that, of the mitotic cells, those in telophase have the highest RNA content, and that the rate of synthesis increases rapidly between anaphase and telophase. These inferences correlate with results obtained from parallel labeled precursor studies.

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