scholarly journals SIMULTANEOUS DIFFERENTIAL STAINING BY A CATIONIC CARBOCYANINE DYE OF NUCLEIC ACIDS, PROTEINS AND CONJUGATED PROTEINS II. CARBOHYDRATE AND SULFATED CARBOHYDRATE-CONTAINING PROTEINS

1974 ◽  
Vol 22 (8) ◽  
pp. 774-781 ◽  
Author(s):  
MARIE R. GREEN ◽  
JULLIA V. PASTEWKA
Keyword(s):  
Mast Cells ◽  
Nucleic Acids ◽  
Hydrogen Ion ◽  
Differential Staining ◽  
Ion Concentrations ◽  
Pathologic Diagnosis ◽  
Carbocyanine Dye ◽  
Sulfated Carbohydrate ◽  
Color Reactions ◽  
Staining Time

The cationic carbocyanine dye l-ethyl-2-[3-(l-ethylnaphtho[l,2d]thiazolin-2-ylidene)-2-methylprolpenyl]-naphtho[1, 2d]thiazolium bromide stains several classes of macromolecules differentially in histologic sections. Most proteins are red at pH 4.3 and pink or unstained at pH 2.8. The caseins are blue at pH 4.3 and unstained at pH 2.8. Nuclei stain purple, mast cells stain red-purple, cartilage stains purple and mucoproteins stain blue-green at both hydrogen ion concentrations. The nature of some of the macromolecules involved in these color reactions has been determined by the use of chemical and enzymatic procedures and by staining films of glycosaminoglycuronoglycans, proteins and conjugated proteins. The method requires less than 1 hr staining time for most tissues; the stain is stable when kept in the dark and has the advantage of distinguishing several macromolecules in tissues simultaneously. The simplicity of the method and the ability to discriminate classes of macromolecules in tissues should make this stain valuable in pathologic diagnosis.

scholarly journals SIMULTANEOUS DIFFERENTIAL STAINING BY A CATIONIC CARBOCYANINE DYE OF NUCLEIC ACIDS, PROTEINS AND CONJUGATED PROTEINS I. PHOSPHOPROTEINS

1974 ◽  
Vol 22 (8) ◽  
pp. 767-773 ◽  
Author(s):  
MARIE R. GREEN ◽  
JULLIA V. PASTEWKA
Keyword(s):  
Nucleic Acids ◽  
Absorption Maximum ◽  
Mammary Glands ◽  
The Other ◽  
Hydrogen Ion ◽  
Differential Staining ◽  
Ion Concentrations ◽  
Carbocyanine Dye ◽  
Pregnant Mice ◽  
Multiple Interactions

The cationic carbocyanine dye l-ethyl-2-[3-(l-ethylnaphtho[l,2d]thiazolin-2-ylidene)-2-methylpropenyl]-naphtho[1,2d]thiazolium bromide interacts in different ways with several classes of polymeric anions in solution. This results in changes in absorption maxima which differ from the absorption maximum of the dye solution. The multiple interactions with macromolecules were demonstrated in histologic sections. Under appropriate electrolyte, dye and hydrogen ion concentrations, nucleic acids, proteins and conjugated proteins were distinguished from each other by color. The phosphoproteins stained blue, proteins red, nucleic acids purple and mucoproteins and mucopolysaccharides various colors. Further discrimination was obtained by chemical procedures and enzymatic digestions. Secretions in alveolar and ductal lumina in the mammary glands of pregnant mice were shown to stain differentially from the other macromolecules. It is inferred on the basis of enzymatic and chemical procedures that the secretions stained blue because of their phosphoprotein content. Conventional staining procedures did not allow this distinction to be made.

scholarly journals Simultaneous differential staining of nucleic acids, proteins, conjugated proteins and polar lipids by a cationic carbocyanine dye.

1975 ◽  
Vol 23 (6) ◽  
pp. 411-423 ◽  
Author(s):  
M R Green
Keyword(s):  
Nucleic Acids ◽  
Polar Lipids ◽  
Differential Staining ◽  
Highly Sensitive ◽  
Carbocyanine Dye ◽  
Chloroform Methanol ◽  
Multiple Interactions

The multiple interactions of the cationic carbocyanine dye, 1-ethyl-naptho-[1,2d]thiazolin-2-ylidene)-2-methylpropenyl]naptho[1,2d]thiazolium bromide, 'Stains-described. Many of these substances could be distinguished from one another on the basis of color in conjunction with chemical and enzymatic digestions. Further studies with this dye have shown that under certain conditions polar lipids as well may be distinguished from these substances. Stains-all has a highly sensitive metachromatic reaction for the presence of polar lipids. It is possible to detect the lipids in large part because they are green and contrast with a red- or pink-stained protein background and with the blue-purple of nuclei or cartilage. Where other green substances occur as in sialoglycoproteins of mucous or membranes, the lipids can be distinguished because they are extracted by chloroform-methanol (2:1) or pyridine.

scholarly journals The acidity of muscle during maintained contraction

1922 ◽  
Vol 93 (654) ◽  
pp. 406-410
Keyword(s):  
Manganese Dioxide ◽  
Hydrogen Ion ◽  
Ion Concentrations

In 1913, I described a method for recording changes in hydrogen-ion concentrations in tissues, by means of a manganese dioxide electrode in combination with a calomel electrode (1). By this method it was shown that the acidity of muscle probably increased at the same time as, or slightly before, the tension increased, and that the acidity decreased as the muscle relaxed (2). In a paper, which appeared as this note was being prepared for publication, Ritchie states that he has been unable to detect a variation in acidity by the use of manganese dioxide electrodes. I am inclined to think that his failure is due to the injury to the muscles on insertion of wires into its substance. In my own experiments the wires rest on the surface of the muscle.

The host defense peptide LL-37 triggers release of nucleic acids from human mast cells

Peptides ◽  
2018 ◽  
Vol 109 ◽  
pp. 39-45 ◽  
Author(s):  
Sara Dahl ◽  
Emma Anders ◽  
Olof Gidlöf ◽  
Daniel Svensson ◽  
Bengt-Olof Nilsson
Keyword(s):  
Mast Cells ◽  
Nucleic Acids ◽  
Host Defense ◽  
Human Mast Cells ◽  
Host Defense Peptide
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