Expression and Regulation of Antimicrobial Peptide Psoriasin (S100A7) at the Ocular Surface and in the Lacrimal Apparatus

2011 ◽  
Vol 52 (7) ◽  
pp. 4914 ◽  
Author(s):  
Fabian Garreis ◽  
Maria Gottschalt ◽  
Thomas Schlorf ◽  
Regine Gläser ◽  
Jürgen Harder ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Ocular Surface ◽  
Lacrimal Apparatus

scholarly journals Expression and Regulation of S100 Fused-Type Protein Hornerin at the Ocular Surface and Lacrimal Apparatus

2017 ◽  
Vol 58 (13) ◽  
pp. 5968 ◽  
Author(s):  
Fabian Garreis ◽  
Janine Jahn ◽  
Katharina Wild ◽  
Daniel B. Abrar ◽  
Martin Schicht ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Lacrimal Apparatus ◽  
Type Protein

Erkrankungen des Tränenapparats, Teil 1: Anatomie, Physiologie und Diagnostik

2020 ◽  
Vol 99 (02) ◽  
pp. 112-125 ◽  
Author(s):  
Jens Heichel ◽  
Hans-Gert Struck ◽  
Arne Viestenz ◽  
Alexander Glien ◽  
Stefan Plontke
Keyword(s):  
Ocular Surface ◽  
Functional Unit ◽  
Inflammatory Diseases ◽  
Congenital Disorders ◽  
Visual Functioning ◽  
Ocular Adnexa ◽  
Lacrimal Apparatus ◽  
Substantial Degree ◽  
Efferent Tear Ducts

AbstractThe lacrimal apparatus is a part of the ocular adnexa preserving ocular surface homoeostasis and therefore enabling sufficiant visual functioning. Tear producting tissues, eyelids and efferent tear ducts operate as one functional unit. Pathologic changes consist of inflammatory diseases, congenital disorders, degenerations and neoplasia. Common to all these conditions is a potential impairment of the ocular integrity as well as a substantial degree of suffering for the affected patients. This review reflects basics of the anatomy, physiology, and diagnostics of the lacrimal apparatus.

scholarly journals A Novel Antimicrobial Peptide on the Ocular Surface Shows Decreased Expression in Inflammation and Infection

2008 ◽  
Vol 49 (1) ◽  
pp. 28 ◽  
Author(s):  
Asiya Abedin ◽  
Imran Mohammed ◽  
Andrew Hopkinson ◽  
Harminder S. Dua
Keyword(s):  
Antimicrobial Peptide ◽  
Ocular Surface

scholarly journals In Vitro Activity of Human β-Defensin 2 against Pseudomonas aeruginosa in the Presence of Tear Fluid

2007 ◽  
Vol 51 (11) ◽  
pp. 3853-3860 ◽  
Author(s):  
Ling C. Huang ◽  
Rachel L. Redfern ◽  
Srihari Narayanan ◽  
Rose Y. Reins ◽  
Alison M. McDermott
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Peptide ◽  
Ocular Surface ◽  
Salt Content ◽  
Inhibitory Effect ◽  
Heat Inactivation ◽  
Binding Interaction ◽  
Cast Doubt

ABSTRACT Pseudomonas aeruginosa causes vision-threatening keratitis and is difficult to treat due to emerging resistance. Human β-defensin 2 (hBD-2) is an antimicrobial peptide expressed by ocular surface epithelia with broad-spectrum activity against various pathogens, including P. aeruginosa. The activity of hBD-2 against P. aeruginosa in the presence of human tears or NaCl was studied. In some experiments, tears were heat-inactivated, filtered, and separated into cationic/anionic fractions or mucin MUC5AC was removed by immunoprecipitation before use. Immunoprecipitation was performed to study the interaction between hBD-2 and MUC5AC. hBD-2 activity was reduced by 40 to 90% in the presence of 17.5 to 70% (vol/vol) tears. NaCl reduced hBD-2 activity, but at most it could account for only 36% of the inhibitory effect of tears. Heat inactivation and filtration attenuated the ability of tears to inhibit hBD-2 activity by 65 and 68%, respectively. Anionic tear fractions significantly reduced (86%) the activity of hBD-2, whereas only a 22% reduction was observed with the cationic fractions. In the absence of MUC5AC, the activity of hBD-2 was restored by 64%. Immunoprecipitation studies suggested that the loss of hBD-2 activity in tears is due to a direct binding interaction with MUC5AC. Our data showed that the antimicrobial activity of hBD-2 is sensitive to the presence of human tears and that this is partly due to the salt content and also the presence of MUC5AC. These data cast doubt on the effectiveness of hBD-2 as an antimicrobial peptide, and additional studies are required to conclusively elucidate its role in innate immunity at the ocular surface in vivo.

scholarly journals Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface

Endocrinology ◽  
2013 ◽  
Vol 154 (6) ◽  
pp. 2034-2045 ◽  
Author(s):  
Ulrike Hampel ◽  
Thomas Klonisch ◽  
Saadettin Sel ◽  
Ute Schulze ◽  
Friedrich P. Paulsen
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Cell Lines ◽  
Ocular Surface ◽  
Corneal Wound Healing ◽  
Cognate Receptor ◽  
Lacrimal Apparatus ◽  
Corneal Wound ◽  
And Gender ◽  
And Migration

Abstract Tear fluid is known to contain many different hormones with relevance for ocular surface homeostasis. We studied the presence and functional role of insulin-like factor 3 (INSL3) and its cognate receptor RXFP2 (relaxin/insulin-like family peptide receptor 2) at the ocular surface and in tears. Expression of human INSL3 and RXFP2 was determined in tissues of the ocular surface and lacrimal apparatus; in human corneal (HCE), conjunctival (HCjE), and sebaceous (SC) epithelial cell lines; and in human tears by RT-PCR and ELISA. We investigated effects of human recombinant INSL3 (hrINSL3) on cell proliferation and cell migration and the influence of hrINSL3 on the expression of MMP2, -9, and -13 and TIMP1 and -2 was quantified by real-time PCR and ELISA in HCE, HCjE, and SC cells. We used a C57BL/6 mouse corneal defect model to elucidate the effect of topical application of hrINSL3 on corneal wound healing. INSL3 and RXFP2 transcripts and INSL3 protein were detected in all tissues and cell lines investigated. Significantly higher concentrations of INSL3 were detected in tears from male vs. female volunteers. Stimulation of HCE, HCjE, and SC with hrINSL3 significantly increased cell proliferation in HCjE and SC and migration of HCjE. Treatment with hrINSL3 for 24 hours regulated MMP2, TIMP1, and TIMP2 expression. The local application of hrINSL3 onto denuded corneal surface resulted in significantly accelerated corneal wound healing in mice. These findings suggest a novel and gender-specific role for INSL3 and cognate receptor RXFP2 signaling in ocular surface homeostasis and determined a novel role for hrINSL3 in corneal wound healing.

The Development of an Antimicrobial Contact Lens – From the Laboratory to the Clinic

2020 ◽  
Vol 21 (4) ◽  
pp. 357-368 ◽  
Author(s):  
Mark D.P. Willcox ◽  
R. Chen ◽  
P. Kalaiselvan ◽  
M. Yasir ◽  
R. Rasul ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Contact Lens ◽  
Ocular Surface ◽  
Contact Lenses ◽  
Research Progress ◽  
Clinical Testing ◽  
Contact Lens Wear ◽  
Loss Of Vision ◽  
Infection And Inflammation ◽  
Lens Wear

Contact lens wear is generally safe and provides excellent vision. However, contact lens wear is often associated with the risk of developing ocular surface infection and inflammation, and in severe cases, the infection can result in loss of vision. Antimicrobial peptide-coated contact lenses have been made to help reduce the incidence of infection and inflammation. This paper reviews the research progress from conception, through the laboratory and preclinical tests to the latest information on clinical testing of an antimicrobial contact lens. We provide insights into the pathways followed and pitfalls that have been encountered. The journey has not always been linear or smooth, but has resulted in some of the first published clinical testing of antimicrobial peptide-coated contact lenses in humans. We hope this may help lead to the development and commercialisation of antimicrobial contact lenses in the future.

Erkrankungen des Tränenapparats, Teil 2: Grundlagen der Therapie

2020 ◽  
Vol 99 (04) ◽  
pp. 247-261
Author(s):  
Jens Heichel ◽  
Hans-Gert Struck ◽  
Arne Viestenz ◽  
Alexander Glien ◽  
Stefan Plontke
Keyword(s):  
Ocular Surface ◽  
Tear Film ◽  
Surgical Approaches ◽  
Therapeutic Approaches ◽  
Film Distribution ◽  
Lacrimal Apparatus ◽  
Therapeutic Principles ◽  
Acute Exacerbations ◽  
Lacrimal Apparatus Diseases ◽  
Duct Stenosis

AbstractThe tearing eye (epiphora) is deemed to be the leading symptome of efferent tear duct stenosis. Nevertheless, epiphora might be caused by ocular surface pathologies or even intraocular diseases. A distinguished anamnesis and sufficient clinical examination is most meaningful for the differential diagnostic distinction. Therapy is based on pathologic changes. In case of ocular surface disease, a suitable tear substitution and antiinflammatory approach is mandatory. Multifarious triggers have to be considered as well. Addressing tear film distribution, suitable oculoplastic surgery is required. Efferent tear duct stenosis necessitates surgical treatment as the level of suffering is often very high and acute exacerbations may develop. Besides recanalization, anastomosing techniques represent the available therapeutic principles. Possible surgical approaches offer a great variety and many influencing factors navigate therapy decisions. This part of the review comments on the most important therapeutic approaches for lacrimal apparatus diseases.

The Spectrum of Antimicrobial Peptide Expression at the Ocular Surface

2005 ◽  
Vol 46 (4) ◽  
pp. 1379 ◽  
Author(s):  
Richard S. McIntosh ◽  
Jennie E. Cade ◽  
Mashael Al-Abed ◽  
Vijay Shanmuganathan ◽  
Rajen Gupta ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Ocular Surface ◽  
Peptide Expression ◽  
Antimicrobial Peptide Expression

scholarly journals Ocular Surface as Barrier of Innate Immunity

2015 ◽  
Vol 9 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Rodrigo Bolaños-Jiménez ◽  
Alejandro Navas ◽  
Erika Paulina López-Lizárraga ◽  
Francesc March de Ribot ◽  
Alexandra Peña ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immunity ◽  
Ocular Surface ◽  
Complex Structure ◽  
Molecular Characteristics ◽  
Human Beings ◽  
Humoral Factors ◽  
Lacrimal Apparatus ◽  
Physical Chemical ◽  
Ocular System

Sight is one of the most important senses that human beings possess. The ocular system is a complex structure equipped with mechanisms that prevent or limit damage caused by physical, chemical, infectious and environmental factors. These mechanisms include a series of anatomical, cellular and humoral factors that have been a matter of study. The cornea is not only the most powerful and important lens of the optical system, but also, it has been involved in many other physiological and pathological processes apart from its refractive nature; the morphological and histological properties of the cornea have been thoroughly studied for the last fifty years; drawing attention in its molecular characteristics of immune response. This paper will review the anatomical and physiological aspects of the cornea, conjunctiva and lacrimal apparatus, as well as the innate immunity at the ocular surface.

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