scholarly journals Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface

Endocrinology ◽  
2013 ◽  
Vol 154 (6) ◽  
pp. 2034-2045 ◽  
Author(s):  
Ulrike Hampel ◽  
Thomas Klonisch ◽  
Saadettin Sel ◽  
Ute Schulze ◽  
Friedrich P. Paulsen
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Cell Lines ◽  
Ocular Surface ◽  
Corneal Wound Healing ◽  
Cognate Receptor ◽  
Lacrimal Apparatus ◽  
Corneal Wound ◽  
And Gender ◽  
And Migration

Abstract Tear fluid is known to contain many different hormones with relevance for ocular surface homeostasis. We studied the presence and functional role of insulin-like factor 3 (INSL3) and its cognate receptor RXFP2 (relaxin/insulin-like family peptide receptor 2) at the ocular surface and in tears. Expression of human INSL3 and RXFP2 was determined in tissues of the ocular surface and lacrimal apparatus; in human corneal (HCE), conjunctival (HCjE), and sebaceous (SC) epithelial cell lines; and in human tears by RT-PCR and ELISA. We investigated effects of human recombinant INSL3 (hrINSL3) on cell proliferation and cell migration and the influence of hrINSL3 on the expression of MMP2, -9, and -13 and TIMP1 and -2 was quantified by real-time PCR and ELISA in HCE, HCjE, and SC cells. We used a C57BL/6 mouse corneal defect model to elucidate the effect of topical application of hrINSL3 on corneal wound healing. INSL3 and RXFP2 transcripts and INSL3 protein were detected in all tissues and cell lines investigated. Significantly higher concentrations of INSL3 were detected in tears from male vs. female volunteers. Stimulation of HCE, HCjE, and SC with hrINSL3 significantly increased cell proliferation in HCjE and SC and migration of HCjE. Treatment with hrINSL3 for 24 hours regulated MMP2, TIMP1, and TIMP2 expression. The local application of hrINSL3 onto denuded corneal surface resulted in significantly accelerated corneal wound healing in mice. These findings suggest a novel and gender-specific role for INSL3 and cognate receptor RXFP2 signaling in ocular surface homeostasis and determined a novel role for hrINSL3 in corneal wound healing.

scholarly journals SFTA3 – a novel surfactant protein of the ocular surface and its role in corneal wound healing and tear film surface tension

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Martin Schicht ◽  
Fabian Garreis ◽  
Nadine Hartjen ◽  
Stephanie Beileke ◽  
Christina Jacobi ◽  
...  
Keyword(s):  
Surface Tension ◽  
Wound Healing ◽  
Ocular Surface ◽  
Tear Film ◽  
Film Surface ◽  
Surfactant Protein ◽  
Corneal Wound Healing ◽  
Corneal Wound

Relaxin 2 Is Functional at the Ocular Surface and Promotes Corneal Wound Healing

2012 ◽  
Vol 53 (12) ◽  
pp. 7780 ◽  
Author(s):  
Ulrike Hampel ◽  
Thomas Klonisch ◽  
Eugenia Makrantonaki ◽  
Saadettin Sel ◽  
Ute Schulze ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ocular Surface ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
Relaxin 2

scholarly journals β-blocker eye drops affect ocular surface through β2 adrenoceptor of corneal limbal stem cells

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xingyue Yuan ◽  
Xiubin Ma ◽  
Lingling Yang ◽  
Qingjun Zhou ◽  
Ya Li
Keyword(s):  
Wound Healing ◽  
Ocular Surface ◽  
Eye Drops ◽  
Limbal Stem Cells ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Epithelial Wound Healing ◽  
Corneal Wound ◽  
Specific Antagonist ◽  
Β Blocker

Abstract Background Topical application of β-blocker eye drops induces damage to the ocular surface in clinical. However, the mechanism involved remains incompletely understood. The purpose of this study was to investigate the influence and mechanism of β-blocker eye drops on corneal epithelial wound healing. Methods Corneal epithelial wound healing models were constructed by epithelial scraping including in the limbal region and unceasingly received eye drops containing 5 mg/mL β-blocker levobunolol, β1-adrenoceptor (β1AR)-specific antagonist atenolol or β2-adrenoceptor (β2AR)-specific antagonist ICI 118, 551. For the migration assay, the murine corneal epithelial stem/progenitor cells (TKE2) were wounded and subsequently incubated with levobunolol, atenolol, or ICI 118, 551. The proliferation and colony formation abilities of TKE2 cells treated with levobunolol, atenolol, or ICI 118, 551 were investigated by CCK-8 kit and crystal violet staining. The differentiation marker Cytokeratin 3 (CK3), the stem cell markers-Cytokeratin 14 (CK14) and Cytokeratin 19 (CK19), and corneal epithelium regeneration-related signaling including in Ki67 and the phosphorylated epithelial growth factor receptor (pEGFR) and phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2) were assessed by immunofluorescence staining. Results Levobunolol and ICI 118, 551 impaired corneal wound healing, decreased the expressions of CK3, CK14, and CK19 after limbal region scraping in vivo and reduced the migration and proliferation of TKE2 in vitro, whereas atenolol had no significant effect. Moreover, levobunolol and ICI 118, 551 inhibited corneal wound healing by mediating the expression of Ki67, and the phosphorylation of EGFR and ERK1/2 in the limbal and regenerated corneal epithelium. Conclusion β-blocker eye drops impaired corneal wound healing by inhibiting the β2AR of limbal stem cells, which decreased corneal epithelial regeneration-related signaling. Therefore, a selective β1AR antagonist might be a good choice for glaucoma treatment to avoid ocular surface damage.

scholarly journals Y-27632, a ROCK Inhibitor, Promoted Limbal Epithelial Cell Proliferation and Corneal Wound Healing

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144571 ◽  
Author(s):  
Chi-Chin Sun ◽  
Hsiao-Ting Chiu ◽  
Yi-Fang Lin ◽  
Kuo-Ying Lee ◽  
Jong-Hwei Su Pang
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Epithelial Cell ◽  
Epithelial Cell Proliferation ◽  
Rock Inhibitor ◽  
Corneal Wound Healing ◽  
Corneal Wound

Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sushmitha Sriramulu ◽  
Antara Banerjee ◽  
Ganesan Jothimani ◽  
Surajit Pathak
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Umbilical Cord ◽  
Human Keratinocytes ◽  
Human Umbilical Cord ◽  
Hacat Cells ◽  
And Migration

AbstractObjectivesWound healing is a complex process with a sequence of restoring and inhibition events such as cell proliferation, differentiation, migration as well as adhesion. Mesenchymal stem cells (MSC) derived conditioned medium (CM) has potent therapeutic functions and promotes cell proliferation, anti-oxidant, immunosuppressive, and anti-apoptotic effects. The main aim of this research is to study the role of human umbilical cord-mesenchymal stem cells (UC-MSCs) derived CM in stimulating the proliferation of human keratinocytes (HaCaT).MethodsFirstly, MSC were isolated from human umbilical cords (UC) and the cells were then cultured in proliferative medium. We prepared and collected the CM after 72 h. Morphological changes were observed after the treatment of HaCaT cells with CM. To validate the findings, proliferation rate, clonal efficiency and also gene expression studies were performed.ResultsIncreased proliferation rate was observed and confirmed with the expression of Proliferating Cell Nuclear Antigen (PCNA) after treatment with HaCaT cells. Cell-cell strap formation was also observed when HaCaT cells were treated with CM for a period of 5–6 days which was confirmed by the increased expression of Collagen Type 1 Alpha 1 chain (Col1A1).ConclusionsOur results from present study depicts that the secretory components in the CM might play a significant role by interacting with keratinocytes to promote proliferation and migration. Thus, the CM stimulates cellular proliferation, epithelialization and migration of skin cells which might be the future promising application in wound healing.

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