scholarly journals A Novel Antimicrobial Peptide on the Ocular Surface Shows Decreased Expression in Inflammation and Infection

2008 ◽  
Vol 49 (1) ◽  
pp. 28 ◽  
Author(s):  
Asiya Abedin ◽  
Imran Mohammed ◽  
Andrew Hopkinson ◽  
Harminder S. Dua
Keyword(s):  
Antimicrobial Peptide ◽  
Ocular Surface

Expression and Regulation of Antimicrobial Peptide Psoriasin (S100A7) at the Ocular Surface and in the Lacrimal Apparatus

2011 ◽  
Vol 52 (7) ◽  
pp. 4914 ◽  
Author(s):  
Fabian Garreis ◽  
Maria Gottschalt ◽  
Thomas Schlorf ◽  
Regine Gläser ◽  
Jürgen Harder ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Ocular Surface ◽  
Lacrimal Apparatus

scholarly journals In Vitro Activity of Human β-Defensin 2 against Pseudomonas aeruginosa in the Presence of Tear Fluid

2007 ◽  
Vol 51 (11) ◽  
pp. 3853-3860 ◽  
Author(s):  
Ling C. Huang ◽  
Rachel L. Redfern ◽  
Srihari Narayanan ◽  
Rose Y. Reins ◽  
Alison M. McDermott
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Peptide ◽  
Ocular Surface ◽  
Salt Content ◽  
Inhibitory Effect ◽  
Heat Inactivation ◽  
Binding Interaction ◽  
Cast Doubt

ABSTRACT Pseudomonas aeruginosa causes vision-threatening keratitis and is difficult to treat due to emerging resistance. Human β-defensin 2 (hBD-2) is an antimicrobial peptide expressed by ocular surface epithelia with broad-spectrum activity against various pathogens, including P. aeruginosa. The activity of hBD-2 against P. aeruginosa in the presence of human tears or NaCl was studied. In some experiments, tears were heat-inactivated, filtered, and separated into cationic/anionic fractions or mucin MUC5AC was removed by immunoprecipitation before use. Immunoprecipitation was performed to study the interaction between hBD-2 and MUC5AC. hBD-2 activity was reduced by 40 to 90% in the presence of 17.5 to 70% (vol/vol) tears. NaCl reduced hBD-2 activity, but at most it could account for only 36% of the inhibitory effect of tears. Heat inactivation and filtration attenuated the ability of tears to inhibit hBD-2 activity by 65 and 68%, respectively. Anionic tear fractions significantly reduced (86%) the activity of hBD-2, whereas only a 22% reduction was observed with the cationic fractions. In the absence of MUC5AC, the activity of hBD-2 was restored by 64%. Immunoprecipitation studies suggested that the loss of hBD-2 activity in tears is due to a direct binding interaction with MUC5AC. Our data showed that the antimicrobial activity of hBD-2 is sensitive to the presence of human tears and that this is partly due to the salt content and also the presence of MUC5AC. These data cast doubt on the effectiveness of hBD-2 as an antimicrobial peptide, and additional studies are required to conclusively elucidate its role in innate immunity at the ocular surface in vivo.

The Development of an Antimicrobial Contact Lens – From the Laboratory to the Clinic

2020 ◽  
Vol 21 (4) ◽  
pp. 357-368 ◽  
Author(s):  
Mark D.P. Willcox ◽  
R. Chen ◽  
P. Kalaiselvan ◽  
M. Yasir ◽  
R. Rasul ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Contact Lens ◽  
Ocular Surface ◽  
Contact Lenses ◽  
Research Progress ◽  
Clinical Testing ◽  
Contact Lens Wear ◽  
Loss Of Vision ◽  
Infection And Inflammation ◽  
Lens Wear

Contact lens wear is generally safe and provides excellent vision. However, contact lens wear is often associated with the risk of developing ocular surface infection and inflammation, and in severe cases, the infection can result in loss of vision. Antimicrobial peptide-coated contact lenses have been made to help reduce the incidence of infection and inflammation. This paper reviews the research progress from conception, through the laboratory and preclinical tests to the latest information on clinical testing of an antimicrobial contact lens. We provide insights into the pathways followed and pitfalls that have been encountered. The journey has not always been linear or smooth, but has resulted in some of the first published clinical testing of antimicrobial peptide-coated contact lenses in humans. We hope this may help lead to the development and commercialisation of antimicrobial contact lenses in the future.

The Spectrum of Antimicrobial Peptide Expression at the Ocular Surface

2005 ◽  
Vol 46 (4) ◽  
pp. 1379 ◽  
Author(s):  
Richard S. McIntosh ◽  
Jennie E. Cade ◽  
Mashael Al-Abed ◽  
Vijay Shanmuganathan ◽  
Rajen Gupta ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Ocular Surface ◽  
Peptide Expression ◽  
Antimicrobial Peptide Expression

The Human Endogenous Antimicrobial Peptide Ll-37 Stimulates Airway Epithelial Repair

Pneumologie ◽  
2006 ◽  
Vol 59 (12) ◽  
Author(s):  
R Shaykhiev ◽  
C Beißwenger ◽  
K Kändler ◽  
J Senske ◽  
A Püchner ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Airway Epithelial ◽  
Epithelial Repair

Profilometry. Using Corneo-scleral topography for scleral lens fitting

The Eye ◽  
2019 ◽  
Vol 21 (128) ◽  
pp. 19-22
Author(s):  
Gregory DeNaeyer
Keyword(s):  
Ocular Surface ◽  
World Wide ◽  
Contact Lenses ◽  
The Past ◽  
The World ◽  
The Future ◽  
The Way

The world-wide use of scleral contact lenses has dramatically increased over the past 10 year and has changed the way that we manage patients with corneal irregularity. Successfully fitting them can be challenging especially for eyes that have significant asymmetries of the cornea or sclera. The future of scleral lens fitting is utilizing corneo-scleral topography to accurately measure the anterior ocular surface and then using software to design lenses that identically match the scleral surface and evenly vault the cornea. This process allows the practitioner to efficiently fit a customized scleral lens that successfully provides the patient with comfortable wear and improved vision.

Antimicrobial Peptide Prodrugs and Mimetics

2016 ◽  
Author(s):  
Marc Devocelle ◽  
Éanna Forde ◽  
André Schütte ◽  
Andrea Molero-Bondia ◽  
Emer Reeves ◽  
...  
Keyword(s):  
Antimicrobial Peptide

LL-37; The Human Epithelial Antimicrobial Peptide and Innate Immunity System

2015 ◽  
Vol 1 (4) ◽  
pp. 76
Author(s):  
Seyadeh Zahra Sajjadiyan ◽  
Sarah Mohammadinejad ◽  
Leila Hassani
Keyword(s):  
Innate Immunity ◽  
Antimicrobial Peptide
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