scholarly journals Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae

2019 ◽  
Vol Volume 14 ◽  
pp. 3983-3993 ◽  
Author(s):  
Umar Farooq ◽  
Touqeer Ahmad ◽  
Ajmal Khan ◽  
Rizwana Sarwar ◽  
Jazib Shafiq ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Klebsiella Pneumoniae ◽  
Methicillin Resistant

scholarly journals BIOSYNTHESIS, CHARACTERIZATION, BACTERICIDAL AND TOXIC EFFECT OF SILVER NANOPARTICLES USING Klebsiella pneumoniae STRAIN STC1

2020 ◽  
pp. 525-531
Author(s):  
S. Febina Bernice Sharon ◽  
Anitha Ganesan
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Klebsiella Pneumoniae ◽  
Toxic Effect ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Fixed Ratio ◽  
Colour Change ◽  
Cost Effective ◽  
Methicillin Resistant ◽  
Synthesis Of Nanoparticles

Nanotechnology dealt with nanoparticles having a size of 1-100nm used significantly in pharmaceutical science and many other fields. The use of microorganisms in the synthesis of nanoparticles emerged as an eco-friendly method. Klebsiella pneumoniae strain STC1 was isolated from cow’s milk and identified by 16S rRNA sequencing. The biosynthesis was done using the fixed ratio of aqueous solution of the bacterial supernatant and AgNO3 and the colour change was observed which proved the formation of silver nanoparticles. The nanoparticles were characterized by UV-Visible Spectrophotometer, FTIR, XRD and SEM. The nanoparticles were found to have sizes ranging from 15 to 70nm. The antibacterial activity of silver nanoparticles was studied against methicillin resistant Staphylococcus aureus, Clostridium perfringens, Pseudomonas sp. and Proteus sp. The Minimum Inhibitory Concentration of silver nanoparticles was found to be 50µg/ml against methicillin resistant Staphylococcus aureus. The synergistic effect of the silver nanoparticles with commercial antibiotics erythromycin and tetracycline was found to be more evident. The time kill assay and the cytoplasm diffusion assay showed the effectiveness of synthesized silver nanoparticles in the study. The synthesized silver nanoparticles had a great antibacterial potential against methicillin resistant Staphylococcus aureus. The toxic effect of silver nanoparticles revealed that as the concentration of the silver nanoparticles increased, the effect of toxicity increased and the hatching percentage of Artemia cysts decreased. The innovation in this present study is that the biosynthesis of nanoparticles is simple, cost effective, providing good bactericidal and cytotoxic activity and it can represent a future for more therapeutic and pharmacological applications.

Recovery efficiency of two glove-sampling methods

2021 ◽  
pp. 1-3
Author(s):  
Amanda K. Lyons ◽  
Laura J. Rose ◽  
Judith Noble-Wang
Keyword(s):  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Sampling Methods ◽  
Recovery Efficiency ◽  
Methicillin Resistant

Abstract Two methods to sample pathogens from gloved hands were compared: direct imprint onto agar and a sponge-wipe method. The sponge method was significantly better at recovering Clostridiodes difficile spores, and no difference was observed between the methods at 101 inoculum for carbapenemase-producing KPC+ Klebsiella pneumoniae, methicillin-resistant Staphylococcus aureus, and Acinetobacter baumannii.

Silver Nanoparticles Toxicity and Bactericidal Effect Against Methicillin-Resistant Staphylococcus aureus: Nanoscale Does Matter

2009 ◽  
Vol 5 (1-4) ◽  
pp. 2-9 ◽  
Author(s):  
Nilda Vanesa Ayala-Núñez ◽  
Humberto H. Lara Villegas ◽  
Liliana del Carmen Ixtepan Turrent ◽  
Cristina Rodríguez Padilla
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bactericidal Effect ◽  
Methicillin Resistant ◽  
Nanoparticles Toxicity

scholarly journals Anti-Bacterial Effect of Synthesized Silver Nanoparticles using Capsicum Annuum L

2019 ◽  
Vol 7 (1) ◽  
pp. 76-80
Author(s):  
A G Raja Lakshmi ◽  
S Puviyarasu
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Klebsiella Pneumoniae ◽  
Capsicum Annuum ◽  
Bell Pepper ◽  
Capsicum Annuum L ◽  
Bacterial Effect

Medicinal plants are the  sources of medicine. To determine the anti-bacterial effect of synthesized silver nanoparticles using Capsicum on Staphylococcus aureus and Klebsiella pneumoniae. The objective of the study was to determine the effect of different concentration extracts of Capsicum  annuum  (red bell pepper, green bell pepper and yellow bell pepper). The extract from Capsicum showed a higher antibacterial activity against  Klebsiella pneumoniae followed by Staphylococcus aureus. The extract had higher percentages of inhibition with increased concentration  of red and yellow bell pepper.

Enhancing the Bioavailability of Silver Through Nanotechnology Approaches Could Overcome Efflux Pump Mediated Silver Resistance in Methicillin Resistant Staphylococcus aureus

2019 ◽  
Vol 15 (11) ◽  
pp. 2216-2228 ◽  
Author(s):  
Saji George ◽  
Ignacius Tay ◽  
Wut Hmone Phue ◽  
Hannah Gardner ◽  
Bindu Sukumaran
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Efflux Pump ◽  
Wide Spectrum ◽  
Consumer Products ◽  
Bacterial Reduction ◽  
Ethylene Imine ◽  
Methicillin Resistant ◽  
Membrane Rupture

While the wide-spectrum antimicrobial properties and stability of silver nanomaterials have been copiously utilized in many medical and consumer products, we found that Methicillin Resistant Staphylococcus aureus (MRSA) is less susceptible to silver in comparison to Methicillin Sensitive Staphylococcus aureus (MSSA). Pre-exposure of MRSA to sub-lethal concentrations of AgNO3 caused 2.5-fold increase in LD50 of silver suggesting an inducible resistance mechanism. Studies involving gene expression profiling and efflux pump blockers showed the induction of P-type efflux pumps (Cop A, Cop Z and Nor B) as the principle mechanism conferring silver resistance in MRSA. Chlorpromazine—an efflux pump blocker increased sensitivity of MRSA to silver. Leveraging on these observations, silver resistance in MRSA was circumvented by enhancing the bioavailability of silver by cationic functioning of silver nanoparticles or by co-delivering silver together with chlorpromazine. Atomic Force Microscopy showed that poly-ethylene imine (PEI) functionalized silver nanoparticles adhere to bacterial cells which was found to increase the bioavailability, membrane rupture and cell death. The strategy of co-delivery of AgNO3 and chlorpromazine using chitosan-functionalized wormhole silica nanoparticles caused 12 log reduction in bacterial count which was 1000 times higher than bacterial reduction by AgNO3 alone. In short, these studies showed that circumventing antimicrobial resistance in pathogenic bacteria is possible by designed silver nanotechnology.

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