Use of Molecular Beacons in a Simple and Sensitive Fluorescence Assay for CG Methyltransferase Activity

2016 ◽  
Vol 16 (11) ◽  
pp. 11939-11942
Author(s):  
Seyeon Kim ◽  
Jongback Gang
Keyword(s):  
Fluorescence Assay ◽  
Molecular Beacons ◽  
Methyltransferase Activity

Detection of DNA methyltransferase activity using allosteric molecular beacons

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 579-584 ◽  
Author(s):  
Weiting Zhang ◽  
Xiaolong Zu ◽  
Yanling Song ◽  
Zhi Zhu ◽  
Chaoyong James Yang
Keyword(s):  
Dna Methylation ◽  
Dna Methyltransferase ◽  
Molecular Beacon ◽  
Molecular Beacons ◽  
Sensitive Detection ◽  
Methyltransferase Activity ◽  
Mtase Activity ◽  
Adenine Methylation ◽  
Methylation Patterns

Abnormal DNA methylation patterns caused by altered DNA methyltransferase (MTase) activity are closely associated with cancer. Herein, using DNA adenine methylation methyltransferase (Dam MTase) as a model analyte, we designed an allosteric molecular beacon (aMB) for sensitive detection of Dam MTase activity.

A highly sensitive fluorescence assay for methyltransferase activity by exonuclease-aided signal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4636-4641 ◽  
Author(s):  
Feng Tang ◽  
Xi-Wen Xing ◽  
Jie-Mei Chu ◽  
Quan Yuan ◽  
Xiang Zhou ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Fluorescence Assay ◽  
Methyltransferase Activity ◽  
Highly Sensitive ◽  
Dna Adenine Methyltransferase

A highly sensitive fluorescence assay for DNA adenine methyltransferase activity was developed using exonuclease-aided signal amplification.

Ultrasensitive detection of 3′-5′ exonuclease enzymatic activity using molecular beacons

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1081-1087 ◽  
Author(s):  
Xu Wu ◽  
Jiao Chen ◽  
Julia Xiaojun Zhao
Keyword(s):  
Enzymatic Activity ◽  
Fluorescence Assay ◽  
Molecular Beacons ◽  
Ultrasensitive Detection ◽  
Exonuclease Activity

An ultrasensitive and rapid fluorescence assay was developed for the detection of 3′-5′ exonuclease activity using molecular beacons.

Photo-tethered molecular beacons for superior light-induction

2021 ◽  
Author(s):  
Robin Klimek ◽  
Mantian Wang ◽  
Vivien R. McKenney ◽  
Erin M. Schuman ◽  
Alexander Heckel
Keyword(s):  
Molecular Beacons ◽  
Light Induction ◽  
In Cells

Photolabile circularization of molecular beacons via backbone phosphates leads to superior probes to study spatiotemporal aspects of RNA in cells.

Sign in / Sign up

Export Citation Format

Share Document