Ultrasensitive detection of 3′-5′ exonuclease enzymatic activity using molecular beacons

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1081-1087 ◽  
Author(s):  
Xu Wu ◽  
Jiao Chen ◽  
Julia Xiaojun Zhao
Keyword(s):  
Enzymatic Activity ◽  
Fluorescence Assay ◽  
Molecular Beacons ◽  
Ultrasensitive Detection ◽  
Exonuclease Activity

An ultrasensitive and rapid fluorescence assay was developed for the detection of 3′-5′ exonuclease activity using molecular beacons.

scholarly journals Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays

2020 ◽  
Vol 142 (35) ◽  
pp. 15098-15106
Author(s):  
Xu Wang ◽  
Alana F. Ogata ◽  
David R. Walt
Keyword(s):  
Enzymatic Activity ◽  
Single Molecule ◽  
Ultrasensitive Detection

Activation of palindromes on a degradable modular grafting probe enables ultrasensitive detection of microRNAs

2021 ◽  
Author(s):  
Jianguo Xu ◽  
Zhi Li ◽  
Yumei Li ◽  
Yusheng Lu ◽  
Jie Wang
Keyword(s):  
Sybr Green ◽  
Sybr Green I ◽  
Ultrasensitive Detection ◽  
Exonuclease Activity

This work describes a single-stranded degradable modular grafting probe for analyzing microRNA-21. In the system, the exonuclease activity of phi 29 polymerase restrains the Sybr Green I/ssDNA induced background. The...

Graphene oxide enhanced specificity at aptamer and its application to multiplexed enzymatic activity sensing

RSC Advances ◽  
2016 ◽  
Vol 6 (14) ◽  
pp. 11815-11821 ◽  
Author(s):  
Xiaojing Xing ◽  
Xueguo Liu ◽  
Ying Zhou ◽  
Dangdang Xu ◽  
Daiwen Pang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Fluorescence Assay ◽  
Activity Sensing

Based on the finding that sufficient GO could enhance the specificity of an ATP aptamer, we proposed a fluorescence assay for alkaline phosphatase and creatine kinase.

scholarly journals Simple and Convenient G-Quadruplex-Based Turn-On Fluorescence Assay for 3′ → 5′ Exonuclease Activity

2011 ◽  
Vol 83 (2) ◽  
pp. 463-466 ◽  
Author(s):  
Chung-Hang Leung ◽  
Daniel Shiu-Hin Chan ◽  
Bradley Yat-Wah Man ◽  
Chuan-Jen Wang ◽  
Wing Lam ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Exonuclease Activity ◽  
Turn On ◽  
G Quadruplex

Branched RCA coupled with a NESA-based fluorescence assay for ultrasensitive detection of miRNA

2017 ◽  
Vol 41 (13) ◽  
pp. 5355-5361 ◽  
Author(s):  
Chenggong Xu ◽  
Xu Wang ◽  
Hui Li ◽  
Cong Han ◽  
Jingfeng Wang ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Ultrasensitive Detection ◽  
First Report

This work is the first report that branched-RCA coupled with NESA has been used for fluorescence assay of miRNA.

A graphene oxide-based fluorescence assay for the sensitive detection of DNA exonuclease enzymatic activity

The Analyst ◽  
2019 ◽  
Vol 144 (21) ◽  
pp. 6231-6239 ◽  
Author(s):  
Xiao Liu ◽  
Yingfen Wu ◽  
Xu Wu ◽  
Julia Xiaojun Zhao
Keyword(s):  
Graphene Oxide ◽  
Enzymatic Activity ◽  
Limit Of Detection ◽  
Fluorescent Sensor ◽  
Fluorescence Assay ◽  
Sensitive Detection ◽  
Exo Iii

A simple and ultra-sensitive fluorescent sensor was developed to detect the enzymatic activity of Exo III with a limit of detection of 0.001 U mL−1.

Ultrasensitive detection of glutathione based on a switch-on fluorescent probe of AIE-type red-emitting copper nanoclusters

2019 ◽  
Vol 11 (27) ◽  
pp. 3446-3451 ◽  
Author(s):  
Qianqian Du ◽  
Xue Hu ◽  
Xiaodan Zhang ◽  
Haiyan Cao ◽  
Yuming Huang
Keyword(s):  
Fluorescent Probe ◽  
High Stability ◽  
Stokes Shift ◽  
Fluorescence Assay ◽  
Fluorescence Recovery ◽  
Ultrasensitive Detection ◽  
Copper Nanoclusters ◽  
Large Stokes Shift

AIE-type red-emitting Cu NCs (with a large stokes shift and high stability) are quenched with MnO2nanosheetsviaIFE, while further introduction of GSH leads to fluorescence recovery, and this finding results in a new switch-on FL fluorescence assay for ultrasensitive detection of GSH.

Ultrasensitive Detection of Enzymatic Activity with Nanowire Electrodes

2007 ◽  
Vol 129 (37) ◽  
pp. 11356-11357 ◽  
Author(s):  
Marcel A. Roberts ◽  
Shana O. Kelley
Keyword(s):  
Enzymatic Activity ◽  
Ultrasensitive Detection
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