Highly sensitive fluorescence assay of DNA methyltransferase activity via methylation-sensitive cleavage coupled with nicking enzyme-assisted signalamplification

2013 ◽  
Vol 42 ◽  
pp. 56-61 ◽  
Author(s):  
Yongxi Zhao ◽  
Feng Chen ◽  
Yayan Wu ◽  
Yanhua Dong ◽  
Chunhai Fan
Keyword(s):  
Dna Methyltransferase ◽  
Fluorescence Assay ◽  
Methyltransferase Activity ◽  
Highly Sensitive ◽  
Nicking Enzyme

Viral cDNA-based extension for highly sensitive fluorescence detection of DNA methyltransferase activity

2018 ◽  
Vol 255 ◽  
pp. 3488-3494 ◽  
Author(s):  
Ying Wang ◽  
Binxiao Li ◽  
Ningbo Zhang ◽  
Dongmei Xi ◽  
Jing Liu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Dna Methyltransferase ◽  
Methyltransferase Activity ◽  
Highly Sensitive ◽  
Viral Cdna

A highly sensitive fluorescence assay for methyltransferase activity by exonuclease-aided signal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4636-4641 ◽  
Author(s):  
Feng Tang ◽  
Xi-Wen Xing ◽  
Jie-Mei Chu ◽  
Quan Yuan ◽  
Xiang Zhou ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Fluorescence Assay ◽  
Methyltransferase Activity ◽  
Highly Sensitive ◽  
Dna Adenine Methyltransferase

A highly sensitive fluorescence assay for DNA adenine methyltransferase activity was developed using exonuclease-aided signal amplification.

Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

2014 ◽  
Vol 50 (73) ◽  
pp. 10691-10694 ◽  
Author(s):  
Lijuan Ji ◽  
Zhewei Cai ◽  
Yingdan Qian ◽  
Ping Wu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Restriction Endonuclease ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Site Specific ◽  
Inhibitor Screening ◽  
Selective Approach ◽  
Highly Sensitive

A sensitive and selective approach for the DNA methyltransferase activity assay and MTase inhibitor screening is reported.

Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation spectroscopy with polystyrene polymer dots

The Analyst ◽  
2021 ◽  
Author(s):  
Yuyang Huang ◽  
Liyun Deng ◽  
Di Su ◽  
Xiangyi Huang ◽  
Jicun Ren
Keyword(s):  
Dna Methyltransferase ◽  
Cell Function ◽  
Vital Role ◽  
Correlation Spectroscopy ◽  
Methyltransferase Activity ◽  
Fluorescence Correlation ◽  
Genetic Imprinting ◽  
Highly Sensitive ◽  
Polymer Dots ◽  
Highly Sensitive Detection

DNA methylation is a critical part of epigenetics and plays a vital role in maintaining normal cell function, genetic imprinting, and human tumorigenesis. Thus, it is important to develop a...

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