Eco-Pap: The Ecological Modification of the Papanicolaou Stain for Sustainable Cervical Cancer Diagnosis

2018 ◽  
Vol 63 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Jeel Moya-Salazar ◽  
Víctor Rojas-Zumaran
Keyword(s):  
Cervical Cancer ◽  
Staining Method ◽  
Nuclear Staining ◽  
Mercury Oxide ◽  
Carcinogenic Potential ◽  
Mother And Child ◽  
Orange G ◽  
Bethesda System ◽  
A Prospective Study ◽  
The Bethesda System

Objectives: To validate the ecological modification of the Papanicolaou stain (Eco-Pap) for the diagnosis of cervical cancer. Study Design: A prospective study was performed at the Mother and Child “San Bartolomé” Hospital, Lima, Peru. Reagent handling strategies were divided into three phases: we used (1) Harris progressive hematoxylin (for nuclear staining), (2) a polychromatic solution (a mix of EA-36/Orange G-6 to suppress the use of several alcohol baths), and (3) direct mounting (with Entellan® solution). The cellular details were analyzed by the staining quality index, an external quality control, and the Bethesda System 2014. Results: We evaluated 72,901 cervical smears stained with the Eco-Pap. The validation of the Eco-Pap against the conventional Pap stain was optimal (κ = 0.89; 95% CI: 0.87–0.92), showing a sensibility and specificity of 88.3% (95% CI: 85.1–90.0) and 98.7% (95% CI: 98–99.2), respectively. The Eco-Pap dramatically reduced the environmental pollution caused by 72 L of xylene, hydrochloric acid, and ammonia (6 L each) and mercury oxide. Conclusion: The Eco-Pap is an innovative and efficient staining method reducing the use of toxic reagents with carcinogenic potential during cervical cancer screening by exfoliative cytology.

Validation of the Modification of the Prolonged Papanicolaou Stain for the Diagnosis of Cervical Cancer

2016 ◽  
Vol 60 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Jeel Moya-Salazar ◽  
Victor Rojas-Zumaran
Keyword(s):  
Quality Control ◽  
Cost Benefit ◽  
Quality Criteria ◽  
Pap Smears ◽  
Orange G ◽  
Cost Benefit Ratio ◽  
Bethesda System ◽  
Cytological Characteristics ◽  
A Prospective Study ◽  
The Bethesda System

Objectives: To validate the modification of the prolonged Papanicolaou stain based on the quality control of time, units and staining stages (micro- and macroscopic), and on the stain reactivation based on the control. Study Design: A prospective study was performed on 125,358 cervical smears in Lima (Peru). The microscopic quality control included evaluating the cytological characteristics through the staining quality index (SQI) and the quality criteria for samples described by the Bethesda System (TBS). The macroscopic quality control is based on the interslide staining as a set corresponding to a poststaining vertical angle. The stains were reactivated with the addition of 0.15 and 2 g of phosphotungstic acid for Orange G and EA-50, respectively. Results: Both the nonneoplastic and the neoplastic findings comply with the quality criteria of the TBS (SQI value = 0.94). The yield per battery of staining fluctuated between 5,616 and 17,954 Pap smears, and the reactivation of stains produced a stain yield of up to four times the initial amount of the smears, saving USD 5,598.88 from 2013 to 2014. Conclusion: The modification of prolonged Papanicolaou staining is an excellent alternative, with a cost-benefit ratio that generates savings and a higher yield per battery of staining.

The cytologic conclusion in accordance a terminology system The Bethesda system 2014: what’s new?

2017 ◽  
pp. 82-84
Author(s):  
O.V. Rykovа ◽  
◽  
T.P. Bogomaz ◽  
Keyword(s):  
Cervical Cancer ◽  
Key Words ◽  
Cervical Screening ◽  
Pap Test ◽  
Bethesda System ◽  
Liquid Based Cytology ◽  
The Bethesda System ◽  
Terminology System

The paper presents the principles of the conclusions Pap test in accordance with the terminology system Bethesda, 2014, and their correlation with other classifications cytological findings. Key words: cervical cancer, cervical screening, traditional cytology, liquid-based cytology, PAP-test, The Bethesda system.

scholarly journals Adequacy of cervical cytology by The Bethesda System cell number in specimens in cervical cancer mass screening

2012 ◽  
Vol 51 (2) ◽  
pp. 110-115 ◽  
Author(s):  
Yutaka MORIMURA ◽  
Seiko CHIBA ◽  
Yukari ARAKI ◽  
Yoshinori SOETA ◽  
Takashi TSUKAHARA ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Mass Screening ◽  
Cell Number ◽  
Cervical Cytology ◽  
System Cell ◽  
Bethesda System ◽  
The Bethesda System

scholarly journals The Bethesda System for Reporting Cervical Cytology: A Historical Perspective

2017 ◽  
Vol 61 (4-5) ◽  
pp. 359-372 ◽  
Author(s):  
Ritu Nayar ◽  
David C. Wilbur
Keyword(s):  
Cervical Cancer ◽  
Cancer Screening ◽  
Cervical Cancer Screening ◽  
Research Management ◽  
Cervical Cytology ◽  
Cervical Disease ◽  
Standardized Terminology ◽  
Bethesda System ◽  
The Bethesda System ◽  
Further Development

The aims of The Bethesda System (TBS) were to provide effective communication from the laboratory to the clinical provider; facilitate cytologic-histologic correlation; facilitate research into the epidemiology, biology, and pathology of cervical disease; and provide reproducible and reliable data for national and international statistical analysis comparisons. Dr. Diane Solomon and colleagues' contribution to cervical cancer screening, diagnosis, and management began with the inception and dissemination of TBS for reporting cervical cytology in 1988, as detailed in the accompanying article [Solomon et al.: Acta Cytol 1989;33:567-574]. The significance of TBS for the further development and implementation of standardized terminology in pathology, and the research/management of cervical cancer have continued to evolve over the past three decades. TBS has always been a multidisciplinary effort and acknowledgement needs to be given to several stakeholders who, over the years, have contributed to its success. It has been our privilege and honor to have carried on the legacy of this seminal work, even as molecular methods are being closely integrated into cervical cancer screening, triage, and prevention.

The cytologic conclusion in accordance a terminology system The Bethesda system 2014: what’s new?

2017 ◽  
pp. 62-64
Author(s):  
O.V. Rykovа ◽  
◽  
T.P. Bogomaz ◽  
Keyword(s):  
Cervical Cancer ◽  
Key Words ◽  
Cervical Screening ◽  
Pap Test ◽  
Bethesda System ◽  
Liquid Based Cytology ◽  
The Bethesda System ◽  
Terminology System

The paper presents the principles of the conclusions Pap test in accordance with the terminology system Bethesda, 2014, and their correlation with other classifications cytological findings. Key words: cervical cancer, cervical screening, traditional cytology, liquid-based cytology, PAP-test, The Bethesda system.

A current applicability of The Bethesda System 2001 in the uterine cervical cancer screening

2011 ◽  
Vol 50 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Takefumi SHIRAYAMA ◽  
Shigeo SEINO ◽  
Noriko KOBAYASHI ◽  
Kouji BANNO ◽  
Takuma FUJII ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Screening ◽  
Cervical Cancer Screening ◽  
Uterine Cervical Cancer ◽  
Bethesda System ◽  
The Bethesda System ◽  
A Current

A PROSPECTIVE STUDY FOR REPORTING THYROID FINE NEEDLE ASPIRATES BY THE BETHESDA SYSTEM WITH HISTOLOGICAL CORRELATION

2021 ◽  
pp. 67-70
Author(s):  
Sankha Chatterjee ◽  
Santosh Kumar Mondal
Keyword(s):  
Prospective Study ◽  
Histopathological Examination ◽  
Needle Aspiration ◽  
Follicular Neoplasm ◽  
Fine Needle Aspirates ◽  
Bethesda System ◽  
Needle Aspiration Cytology ◽  
A Prospective Study ◽  
Thyroid Cytopathology ◽  
The Bethesda System

BACKGROUND: To reduce ambiguity in diagnostic terminology in thyroid ne needle aspiration cytology (FNAC),The Bethesda System for Reporting Thyroid cytopathology (TBSRTC) system is a universal reporting system for standardization and could help in assessing the prognosis by estimating the malignant potential of each category . AIMS:The aim of the study is cytodiagnosis of thyroid ne needle aspirates and uniformity in the reporting as per Bethesda system. Histological examination was also conducted so that a correlation between cytodiagnosis and histopathological examination could be made. MATERIALS & METHODS: This prospective study done on patients (125) presenting with thyroid swelling and advised for FNAC by clinicians, diagnosed according to the Bethesda system of thyroid cytopathology reporting . Then, correlation with histopathological report done in cases(55) in which surgery were done. RESULTS:The distribution of cases were as follows: 2 nondiagnostic(1.6%),89 cases(71.2%) were classied as benign, 6 cases (4.8%) were classied as atypia of undetermined signicance /follicular lesion of undetermined signicance/AFLUS, 7 cases(5.6%) were classied as follicular neoplasm/SFN, 3 cases( 2.4% ) were classied as suspicious, and 18 cases( 14.4%) were classied as malignant.In our study, rates of malignancy for each category are : nondiagnostic-0%, benign-4.76%, AFLUS- 16.67% ,SFN-28.57%, suspicious- 66.67% and malignant-94.12%.

The cytologic conclusion in accordance a terminology system The Bethesda system 2014: what's new?

2017 ◽  
pp. 132-136
Author(s):  
O.V. Rykovа ◽  
◽  
T.P. Bogomaz ◽  
Keyword(s):  
Cervical Cancer ◽  
Key Words ◽  
Cervical Screening ◽  
Pap Test ◽  
Bethesda System ◽  
Liquid Based Cytology ◽  
The Bethesda System ◽  
Terminology System

The paper presents the principles of the conclusions Pap test in accordance with the terminology system Bethesda, 2014, and their correlation with other classifications cytological findings. Key words: cervical cancer, cervical screening, traditional cytology, liquid-based cytology, PAP-test, The Bethesda system.

scholarly journals Discrimination of cervical cancer cells via cognition-based features

2019 ◽  
Vol 13 (01) ◽  
pp. 2050001
Author(s):  
Yue Liu ◽  
Jiabo Ma ◽  
Xu Li ◽  
Xiuli Liu ◽  
Gong Rao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cervical Screening ◽  
Computer Assisted ◽  
Cervical Cancer Cells ◽  
Bethesda System ◽  
Cervical Cells ◽  
The Bethesda System ◽  
Nuclear Segmentation

Computer-assisted cervical screening is an effective method to save the doctors’ workload and improve their work efficiency. Usually, the correct classification of cervical cells depends on the nuclear segmentation effect and the extraction of nuclear features. However, the precise nucleus segmentation remains a huge challenge, especially for densely distributed nucleus. Moreover, previous cellular classification methods are mostly based on morphological features of nucleus size or color. Those individual features can make accurate classification for severe lesions, but not for mild lesions. In this paper, we propose an accurate instance segmentation algorithm and propose cognition-based features to identify cervical cancer cells. Different from previous individual nucleus features, we also propose population features and cognition-based features according to the Bethesda System (TBS) for reporting cervical cytology and the diagnostic experience of the cytologists. The results showed that the segmentation achieves better success in complex situations than that by traditional segmentation algorithms. Besides, the cell classification via cognition-based features also help us find out more about less severe lesions’ nuclei than that based on conventional features of individual nucleus, meaning an improvement of classification accuracy for cervical screening.

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