Validation of the Modification of the Prolonged Papanicolaou Stain for the Diagnosis of Cervical Cancer

2016 ◽  
Vol 60 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Jeel Moya-Salazar ◽  
Victor Rojas-Zumaran
Keyword(s):  
Quality Control ◽  
Cost Benefit ◽  
Quality Criteria ◽  
Pap Smears ◽  
Orange G ◽  
Cost Benefit Ratio ◽  
Bethesda System ◽  
Cytological Characteristics ◽  
A Prospective Study ◽  
The Bethesda System

Objectives: To validate the modification of the prolonged Papanicolaou stain based on the quality control of time, units and staining stages (micro- and macroscopic), and on the stain reactivation based on the control. Study Design: A prospective study was performed on 125,358 cervical smears in Lima (Peru). The microscopic quality control included evaluating the cytological characteristics through the staining quality index (SQI) and the quality criteria for samples described by the Bethesda System (TBS). The macroscopic quality control is based on the interslide staining as a set corresponding to a poststaining vertical angle. The stains were reactivated with the addition of 0.15 and 2 g of phosphotungstic acid for Orange G and EA-50, respectively. Results: Both the nonneoplastic and the neoplastic findings comply with the quality criteria of the TBS (SQI value = 0.94). The yield per battery of staining fluctuated between 5,616 and 17,954 Pap smears, and the reactivation of stains produced a stain yield of up to four times the initial amount of the smears, saving USD 5,598.88 from 2013 to 2014. Conclusion: The modification of prolonged Papanicolaou staining is an excellent alternative, with a cost-benefit ratio that generates savings and a higher yield per battery of staining.

Eco-Pap: The Ecological Modification of the Papanicolaou Stain for Sustainable Cervical Cancer Diagnosis

2018 ◽  
Vol 63 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Jeel Moya-Salazar ◽  
Víctor Rojas-Zumaran
Keyword(s):  
Cervical Cancer ◽  
Staining Method ◽  
Nuclear Staining ◽  
Mercury Oxide ◽  
Carcinogenic Potential ◽  
Mother And Child ◽  
Orange G ◽  
Bethesda System ◽  
A Prospective Study ◽  
The Bethesda System

Objectives: To validate the ecological modification of the Papanicolaou stain (Eco-Pap) for the diagnosis of cervical cancer. Study Design: A prospective study was performed at the Mother and Child “San Bartolomé” Hospital, Lima, Peru. Reagent handling strategies were divided into three phases: we used (1) Harris progressive hematoxylin (for nuclear staining), (2) a polychromatic solution (a mix of EA-36/Orange G-6 to suppress the use of several alcohol baths), and (3) direct mounting (with Entellan® solution). The cellular details were analyzed by the staining quality index, an external quality control, and the Bethesda System 2014. Results: We evaluated 72,901 cervical smears stained with the Eco-Pap. The validation of the Eco-Pap against the conventional Pap stain was optimal (κ = 0.89; 95% CI: 0.87–0.92), showing a sensibility and specificity of 88.3% (95% CI: 85.1–90.0) and 98.7% (95% CI: 98–99.2), respectively. The Eco-Pap dramatically reduced the environmental pollution caused by 72 L of xylene, hydrochloric acid, and ammonia (6 L each) and mercury oxide. Conclusion: The Eco-Pap is an innovative and efficient staining method reducing the use of toxic reagents with carcinogenic potential during cervical cancer screening by exfoliative cytology.

Pap Test Reporting Rates for Conventional Smear and Liquid-Based Cervical Cytology from the Largest Academic Women's Hospital in China: Analysis of 1,248,785 Pap Test Reports

2015 ◽  
Vol 59 (6) ◽  
pp. 445-451 ◽  
Author(s):  
Xiang Tao ◽  
R. Marshall Austin ◽  
Hao Zhang ◽  
Lihong Zhang ◽  
Jianan Xiao ◽  
...  
Keyword(s):  
Cervical Screening ◽  
Pap Test ◽  
Major Change ◽  
Cervical Cytology ◽  
Pap Smears ◽  
Pap Tests ◽  
Bethesda System ◽  
Liquid Based Cytology ◽  
The Bethesda System ◽  
Reporting Rates

Objective: The Obstetrics and Gynecology Hospital of Fudan University (OGHFU) in Shanghai is the largest academic women's hospital in China. Between 2009 and 2014, the use of liquid-based cytology (LBC) significantly increased while gradually adopting the Bethesda System (TBS), and in 2012 local regulations mandated that pathologists replace technicians to sign out Pap tests. Design: A retrospective OGHFU database search documented all Pap test reports between 2009 and 2014 by specimen type, either LBC or conventional Pap smears (CPS), and final reporting category. A total of 1,224,785 Pap reports were analyzed to document variations in Pap test reporting during a period of major change in cervical screening in China. Results: LBC gradually replaced CPS, which declined from over 65% of Pap tests in 2010 to 6.4% in 2014. Of 514,811 Pap reports using the traditional class system, class I (negative) reports accounted for 98.3% of results. With the introduction of TBS reporting, pathologist reviews and substantial replacement of CPS by LBC, the laboratory abnormal Pap test rate increased significantly to almost 5%. Conclusions: Changes in cervical cytology reporting between 2009 and 2014 in China's largest academic women's hospital reflected both increased use of LBC and the introduction of pathologist TBS reporting. Abnormality rates increased significantly and fell within CAP benchmark ranges.

Comparison of the diagnosis of uterine cervical (Pap.) smears from Japan and the US-Modified Papanicolaou's classification vs The Bethesda System 2001-

2005 ◽  
Vol 44 (3) ◽  
pp. 111-117 ◽  
Author(s):  
Hidehiro TAKEI ◽  
Bernardo RUIZI ◽  
Maki TAKAHASHI ◽  
Hiroyoshi SUZUKI ◽  
Fumiaki TEZUKA
Keyword(s):  
Pap Smears ◽  
The Us ◽  
Bethesda System ◽  
The Bethesda System

scholarly journals Quality Control for Biomarker Determination in Oncology: The Experience of the Italian Network for Quality Assessment of Tumor Biomarkers (INQAT)

2002 ◽  
Vol 17 (3) ◽  
pp. 201-214 ◽  
Author(s):  
A. Paradiso ◽  
S. Volpe ◽  
A. Iacobacci ◽  
E. Marubini ◽  
P. Verderio ◽  
...  
Keyword(s):  
Quality Control ◽  
Quality Assessment ◽  
National Research Council ◽  
Cost Benefit ◽  
Biomarker Analysis ◽  
Cost Benefit Ratio ◽  
Analytical Phase ◽  
Definition Of ◽  
New Biomarkers ◽  
The Cost

Biomarker analysis and evaluation in oncology is the product of a number of processes (including managerial, technical and interpretation steps) which need to be monitored and controlled to prevent and correct errors and guarantee a satisfactory level of quality. Several biomarkers have recently moved to clinical validation studies and successively to clinical practice without any definition of standard procedures and/or quality control (QC) schemes necessary to guarantee the reproducibility of the laboratory information. In Italy several national scientific societies and single researchers have activated – often on a pilot level – specific external quality assessment protocols, thereby potentially jeopardizing the clinical reality even further. In view of the seriousness of the problem, in 1998 the Italian Ministry of Health sponsored a National Survey Project to coordinate and standardize the procedures and to develop QC programs for the analysis of cancer biomarkers of potential clinical relevance. Twelve QC programs focused on biomarkers and concerning morphological, immunohistochemical, biochemical, molecular, and immunoenzymatic assays were coordinated and implemented. Specifically, external QC programs for the analytical phase of immunohistochemical p53, Bcl-2, c-erb-2/neu/HER2, and microvessel density determination, of morphological evaluation of tumor differentiation grade, and of molecular p53 analysis were activated for the first time within the project. Several hundreds of Italian laboratories took part in these QC programs, the results of which are available on the web site of the Network ( www.cqlaboncologico.it ). Financial support from the Italian Government and the National Research Council (CNR) will guarantee the pursuit of activities that will be extended to new biomarkers, to preanalytical phases of the assays, and to revision of the criteria of clinical usefulness for evaluating the cost/benefit ratio.

Effects of the Bethesda System on the Rate of Unsatisfactory Pap Smears in Spontaneous Cervical Screening

1996 ◽  
Vol 82 (5) ◽  
pp. 437-440 ◽  
Author(s):  
Nori Morini ◽  
Lauro Bucchi ◽  
Carlo Naldoni ◽  
Patrizia Schincaglia ◽  
Nadia Capacci ◽  
...  
Keyword(s):  
Rate Ratio ◽  
Cervical Screening ◽  
Monitoring Program ◽  
Pap Smears ◽  
Foreign Material ◽  
Screening Practice ◽  
Bethesda System ◽  
The Bethesda System ◽  
Substantial Change ◽  
Cytology Report

Aims In 1990, The Bethesda System (TBS) was introduced into spontaneous cervical screening practice in Ravenna, Italy. Negative/benign reports with the recommendation for early repeat smears (RERS) due to some limitation in sample adequacy were considered no longer acceptable. A monitoring program for the rate of unsatisfactory smears (UNS) was implemented. The aim of the present study was to evaluate the effects of such changes in the screening procedure. Methods The frequency of UNS in 1990 was compared with that of UNS+RERS in 1988 (assumed as a baseline year) by the calculation of the standardized rate ratio with the 95% confidence interval (CI). The trend in the standardized rate of UNS from 1990 to 1994 was evaluated by the calculation of the average annual variation with the 95% CI. Results The immediate effect of TBS (1990:1988 comparison) was a significant increase in the rate of UNS attributable to scant cellularity, poor fixation and thick areas (rate ratio, 2.35; 95% CI, 2.18 to 2.53) and to the absence of endocervical component (1.45; 95% CI, 1.30 to 1.60). The rate of UNS attributable to the presence of cytolysis, inflammation, blood and foreign material decreased by about 6 times (0.16; 95% CI, 0.13 to 0.19). The midterm effect of TBS (trend from 1990 to 1994) was a decrease in the total rate of UNS by an average of 2.3% per year. The downward trend was significant for smears showing scant cellularity, poor fixation and thick areas (-1.5% per year) and the absence of endocervical component (-0.7% per year). UNS attributable to the presence of cytolysis, inflammation, blood and foreign material stabilized. Conclusions TBS led to a substantial change in the type of information provided by the cytology report (Immediate effect). The monitoring program according to TBS led to a reduction in UNS attributable to sample taker (midterm effect).

A PROSPECTIVE STUDY FOR REPORTING THYROID FINE NEEDLE ASPIRATES BY THE BETHESDA SYSTEM WITH HISTOLOGICAL CORRELATION

2021 ◽  
pp. 67-70
Author(s):  
Sankha Chatterjee ◽  
Santosh Kumar Mondal
Keyword(s):  
Prospective Study ◽  
Histopathological Examination ◽  
Needle Aspiration ◽  
Follicular Neoplasm ◽  
Fine Needle Aspirates ◽  
Bethesda System ◽  
Needle Aspiration Cytology ◽  
A Prospective Study ◽  
Thyroid Cytopathology ◽  
The Bethesda System

BACKGROUND: To reduce ambiguity in diagnostic terminology in thyroid ne needle aspiration cytology (FNAC),The Bethesda System for Reporting Thyroid cytopathology (TBSRTC) system is a universal reporting system for standardization and could help in assessing the prognosis by estimating the malignant potential of each category . AIMS:The aim of the study is cytodiagnosis of thyroid ne needle aspirates and uniformity in the reporting as per Bethesda system. Histological examination was also conducted so that a correlation between cytodiagnosis and histopathological examination could be made. MATERIALS & METHODS: This prospective study done on patients (125) presenting with thyroid swelling and advised for FNAC by clinicians, diagnosed according to the Bethesda system of thyroid cytopathology reporting . Then, correlation with histopathological report done in cases(55) in which surgery were done. RESULTS:The distribution of cases were as follows: 2 nondiagnostic(1.6%),89 cases(71.2%) were classied as benign, 6 cases (4.8%) were classied as atypia of undetermined signicance /follicular lesion of undetermined signicance/AFLUS, 7 cases(5.6%) were classied as follicular neoplasm/SFN, 3 cases( 2.4% ) were classied as suspicious, and 18 cases( 14.4%) were classied as malignant.In our study, rates of malignancy for each category are : nondiagnostic-0%, benign-4.76%, AFLUS- 16.67% ,SFN-28.57%, suspicious- 66.67% and malignant-94.12%.

scholarly journals Collection of Cervical Secretions Does Not Adversely Affect Pap Smears Taken Immediately Afterward

1998 ◽  
Vol 5 (4) ◽  
pp. 491-493 ◽  
Author(s):  
Allan Hildesheim ◽  
M. Concepcion Bratti ◽  
Robert P. Edwards ◽  
Mark Schiffman ◽  
Ana C. Rodriguez ◽  
...  
Keyword(s):  
Pap Smear ◽  
Inflammatory Cells ◽  
Study Groups ◽  
Pap Smears ◽  
Chi Square ◽  
Transformation Zone ◽  
Chi Square Test ◽  
Bethesda System ◽  
The Bethesda System

ABSTRACT Collection of cervical secretions for local immunological assessment requires that the secretions be collected prior to the Pap smear to avoid contamination with blood. The objective of the present study was to determine whether gentle collection of cervical secretions prior to a Pap smear collection influences the quality of the Pap smear. A total of 266 women were recruited. Half of the participants were assigned to collection of cervical secretions prior to Pap smear collection with Weck-cel sponges. The remaining half had only the Pap smear collection performed. Pap smear slides were reviewed and evaluated for quality by the Bethesda System adequacy criteria without knowledge of randomization. The proportions of limited or inadequate slides in the two study groups were compared by using the Pearson chi-square test. No significant differences were observed between the two study groups when overall Pap smear quality was evaluated (P = 0.29). Comparison of the two study groups with respect to individual adequacy criteria, including presence of air drying artifact, presence of obscuring blood, absence of metaplastic or endocervical cells from the transformation zone, scant cellularity, and presence of obscuring inflammatory cells, also revealed no significant differences between the two study groups. Results from the present study suggest that the collection of cervical secretions with Weck-cel sponges does not adversely impact the quality of subsequently obtained Pap smears.

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