Scanning Electron Microscope Studies of Hepatocytes under Different Experimental Conditions*

2015 ◽  
pp. 42-47
Author(s):  
Juan D. Vial ◽  
Francis R. Simon ◽  
A. Malcolm Mackinnon
2019 ◽  
Vol 295 ◽  
pp. 93-97
Author(s):  
Chang Zhao ◽  
Man Zhao ◽  
Su Ye Lv ◽  
Qing Jun Liu ◽  
Guang Jian Xing

This study prepared an SiC thin film by using the ratio frequency magnetron sputtering method, investigated the effects of different sputtering powers on the SiC material and analysed the changes in crystal morphology and photoluminescence characteristics caused by changes in the growth conditions used. It was considered that there was 6H-SiC crystal morphologies in the SiC thin film under the experimental conditions prevailing in this study. The SiC morphologies with small grain sizes intermingled and therefore formed anSiC thin film. The analyses of the photoluminescence spectra and Scanning Electron Microscope indicated that the SiC thin film materials with preferable crystal compositions could be prepared under appropriate power inputs.


2012 ◽  
Vol 472-475 ◽  
pp. 2756-2759
Author(s):  
Wen Cui ◽  
Shao Jun Qi

To understand the relationship between surface finish and zinc whisker growth, this study investigated the growth of whiskers on two mild steel substrates of different surface finish by Field Emission Gun Scanning Electron Microscope (FEG SEM). Results show that, under the same experimental conditions, deposits on substrates with a mirror finish grew less whiskers and nodules than substrates with a rough surface finish.


2007 ◽  
Vol 33 (2) ◽  
pp. 51-58 ◽  
Author(s):  
Manal M. Shalabi ◽  
Johannes G. C. Wolke ◽  
Anja J. E. de Ruijter ◽  
John A. Jansen

Abstract The aim of the study was to assess the effects of surgical technique and implant surface roughness on implant fixation. A total of 48 screw implants with machined or etched surface topographies were placed into the femoral condyles of goats. The implant sites were prepared by a conventional technique, by undersized preparation, or by the osteotome technique. Bone tissue responses were evaluated after 12 weeks of healing by removal torque testing and histologic analysis using scanning electron microscope. The cumulative removal torque value of the etched implants placed with the undersized technique (98 ± 29 Ncm) was higher (50 ± 35 Ncm) to a level of statistical significance than machined surface implants placed by the osteotome technique. Scanning electron microscope evaluation indicated that all implants showed interfacial bone contact. The torque test resulted in fracture at the bone-implant interface for all experimental conditions. Installation of etched implants using an undersized preparation of the implant bed resulted in superior bonding strength with the surrounding bone at 12 weeks after surgery. Evidently, the undersized preparation technique improved the early fixation of oral implants in this study.


1997 ◽  
Vol 506 ◽  
Author(s):  
J. Giménez ◽  
M. Molera ◽  
I. Casas ◽  
M.E. Torrero ◽  
J. de Pablo

ABSTRACTExperiments of spent fuel dissolution performed in presence of iron show, for uranium as well as for some radionuclides, lower final concentrations than measured in similar experiments but in absence of iron. So far, only the final concentrations obtained in these experiments have been published. In this work, we have focused our study on the reduction of uranium (VI) due to the presence of iron and the subsequent precipitation of Uranium (IV) due to its lower solubility. This behavior has been confirmed using a Scanning Electron Microscope by observing an uranium phase precipitated on the iron surface and assuming, under the experimental conditions, that it most likely corresponds to U02(s). We have modeled this process assuming a first-order kinetic.


2011 ◽  
Vol 17 (5) ◽  
pp. 772-778 ◽  
Author(s):  
Clifford S. Todd ◽  
Valentina Kuznetsova

AbstractClosed cell polymer foam skin thickness can be assessed by taking backscatter electron (BSE) images in a scanning electron microscope (SEM) at a series of accelerating voltages. Under a given set of experimental conditions, the electron beam mostly passes through thin polymer skin cell walls. That cell appears dark compared to adjacent thicker-skinned cells. Higher accelerating voltages lead to a thicker skin being penetrated. Monte Carlo modeling of beam-sample interactions indicates that at 5 keV, skin less than ∼0.5 μm in thickness will appear dark, whereas imaging at 30 keV allows skin thicknesses up to ∼4 μm to be identified. The distribution of skin thickness can be assessed over square millimeters of foam surface in this manner. Qualitative comparisons of the skin thicknesses of samples can be made with a simple visual inspection of the images. A semiquantitative comparison is possible by applying image analysis. The proposed method is applied to two example foams. Characterizing foam skin thickness by this method is possible using any SEM that is capable of collecting useful BSE images over a range of accelerating voltages. Imaging in low vacuum, where an electrically conductive metal coating is not required, leads to more sensitivity in skin thickness characterization.


CONVERTER ◽  
2021 ◽  
pp. 176-189
Author(s):  
Jiangchun Li, Et al.

Objectives: Paper documents are playing an increasingly important role in people's daily work with the development of economy, society and culture. In the practice of judicial appraisal, the sequence of the intersections of ink and seal on suspicious documents can often provide critical information for the detection of criminal cases. The examination of sequence of intersecting seal and ink lines is to judge the sequence of seal and ink mark formation by certain technical means.Methods: A representative black signature pen, ink, and specific paper are selected to prepare experimental samples. Under the given experimental conditions, the field emission scanning electron microscope is used to perform micro-morphology on the cross-sectional characteristics of the samples and all the characterization results obtained are systematically analyzed to summarize the specificity of the sample. Results: The results showed that the proposed method can efficiently discriminate the Permeation Characterization of the sequence of intersecting seal and ink lines.Conclusions: This research is expected to be applied to forensic investigation for counterfeiting documents and bring new developments in the field of document inspection.


Author(s):  
R. E. Ferrell ◽  
G. G. Paulson

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.


Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.


Author(s):  
K. Shibatomi ◽  
T. Yamanoto ◽  
H. Koike

In the observation of a thick specimen by means of a transmission electron microscope, the intensity of electrons passing through the objective lens aperture is greatly reduced. So that the image is almost invisible. In addition to this fact, it have been reported that a chromatic aberration causes the deterioration of the image contrast rather than that of the resolution. The scanning electron microscope is, however, capable of electrically amplifying the signal of the decreasing intensity, and also free from a chromatic aberration so that the deterioration of the image contrast due to the aberration can be prevented. The electrical improvement of the image quality can be carried out by using the fascionating features of the SEM, that is, the amplification of a weak in-put signal forming the image and the descriminating action of the heigh level signal of the background. This paper reports some of the experimental results about the thickness dependence of the observability and quality of the image in the case of the transmission SEM.


Author(s):  
S. Takashima ◽  
H. Hashimoto ◽  
S. Kimoto

The resolution of a conventional transmission electron microscope (TEM) deteriorates as the specimen thickness increases, because chromatic aberration of the objective lens is caused by the energy loss of electrons). In the case of a scanning electron microscope (SEM), chromatic aberration does not exist as the restrictive factor for the resolution of the transmitted electron image, for the SEM has no imageforming lens. It is not sure, however, that the equal resolution to the probe diameter can be obtained in the case of a thick specimen. To study the relation between the specimen thickness and the resolution of the trans-mitted electron image obtained by the SEM, the following experiment was carried out.


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