Adjuvant Activity of the Histamine-Sensitizing Factor of Bordetella pertussis in Different Strains of Mice

1975 ◽  
Vol 49 (6) ◽  
pp. 796-813 ◽  
Author(s):  
Samuel B. Lehrer ◽  
John H. Vaughan ◽  
Eng M. Tan
Keyword(s):  
Bordetella Pertussis ◽  
Adjuvant Activity ◽  
Different Strains

Co-infection with two different strains of Bordetella pertussis in an infant

2008 ◽  
Vol 57 (3) ◽  
pp. 388-391 ◽  
Author(s):  
Pamela K. Cassiday ◽  
Melissa Tobin-D'Angelo ◽  
J. Renee Watson ◽  
Kai-Hui Wu ◽  
Mahin M. Park ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Gene Sequence ◽  
Profile Analysis ◽  
23S Rrna ◽  
Rrna Gene ◽  
Pcr Rflp ◽  
23S Rrna Gene ◽  
History Of ◽  
Simultaneous Infection ◽  
Different Strains

We report co-infection with two phenotypically and genotypically distinct strains of Bordetella pertussis in an infant male hospitalized with a 2-week history of cough, paroxysms and vomiting. Colonies from the two B. pertussis phenotypes were isolated and evaluated by PFGE profile analysis, gene sequence typing and PCR-RFLP of a portion of the 23S rRNA gene. These results demonstrated simultaneous infection with two different strains of B. pertussis.

Variable Adjuvant Activity of Bordetella pertussis with Respect to the Primary and Secondary Immunization of Mice

1970 ◽  
Vol 1 (3) ◽  
pp. 251-258
Author(s):  
H. Finger ◽  
P. Emmerling ◽  
E. Brüss
Keyword(s):  
Immune Responses ◽  
Bordetella Pertussis ◽  
Serum Antibody ◽  
Secondary Response ◽  
Primary Immunization ◽  
Spleen Cells ◽  
Adjuvant Effect ◽  
Adjuvant Activity ◽  
Sheep Erythrocytes ◽  
X Cells

We carried out a study on the adjuvant effect of Bordetella pertussis vaccine on the primary and secondary immune responses of the mouse to sheep erythrocytes, quantitating antibody-producing spleen cells and serum antibody. The simultaneous injection of sheep erythrocytes and B. pertussis , when compared to immunization with sheep red blood cells alone, resulted in an increased and prolonged multiplication of antibody-forming spleen cells. The adjuvant effect was also documented by increased production of serum hemolysins and agglutinins. Further, B. pertussis enhanced the priming effect of the antigen for the secondary response. However, when the bacterial adjuvant was given together with a second antigenic stimulus 41 days after the primary immunization, the peak values of direct and indirect plaque-forming spleen cells did not differ from the corresponding control animals further inoculated with sheep erythrocytes alone. Nonetheless, the influence of the bacterial adjuvant was still expressed by the delayed decrease of the numbers of plaque-forming spleen cells. On the basis of the X-Y-Z scheme it is suggested that B. pertussis cells as adjuvant enhance the multiplication of antigen-sensitive X cells or affect the initial stages of differentiation of these cells. This effect of the pertussis vaccine can be distinguished from a general proliferative action on other cells.

scholarly journals Adjuvant Activity of Free Bordetella Pertussis Filamentous Haemagglutinin Delivered by Mucosal Routes

2003 ◽  
Vol 58 (5) ◽  
pp. 503-510 ◽  
Author(s):  
O. Poulain-Godefroy ◽  
F. D. Menozzi ◽  
S. Alonso ◽  
C. Vendeville ◽  
A. Capron ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Adjuvant Activity

Antigen-sensitive spleen cells and the adjuvant activity of Bordetella pertussis

1970 ◽  
Vol 16 (7) ◽  
pp. 623-627 ◽  
Author(s):  
H. Finger ◽  
P. Emmerling ◽  
M. Büsse
Keyword(s):  
Immune Response ◽  
Bordetella Pertussis ◽  
Primary Immune Response ◽  
Target Cells ◽  
Primary Immunization ◽  
Spleen Cells ◽  
Adjuvant Effect ◽  
Adjuvant Activity ◽  
Simultaneous Injection ◽  
Sheep Erythrocytes

In this study we determined at both the cellular and humoral level whether or not the primary immune response of mice can be significantly enhanced by administration of a bacterial adjuvant after the primary immunization with sheep erythrocytes. As compared to the immunization of mice with 8 × 106 sheep erythrocytes alone, the simultaneous injection of 3 × 109Bordetella pertussis cells and 8 × 106 sheep erythrocytes resulted in an accelerated and prolonged multiplication of hemolysin-forming spleen cells. The adjuvant effect was also documented by increased production of serum hemolysins. When the bacterial adjuvant was given 6, 12, or 24 h after the primary antigenic stimulus, however, neither increased plaque counts nor enhanced serum hemolysin titers were detectable. These findings agree with the concept that B. pertussis cells cause multiplication of antigen-sensitive target cells or affect the initial stages of differentiation of these cells.

Immuno-electron microscopy of surface antigens of Bordetella pertussis

1988 ◽  
Vol 46 ◽  
pp. 314-315
Author(s):  
M. E. Bisher ◽  
M. J. Brennan ◽  
Z. M. Li ◽  
A. C. Steven
Keyword(s):  
Electron Microscopy ◽  
Bordetella Pertussis ◽  
Molecular Level ◽  
Whooping Cough ◽  
Surface Antigens ◽  
Negative Bacterium ◽  
Convenient Means ◽  
Immuno Electron Microscopy ◽  
Serotype 1 ◽  
Different Strains

Bordetella pertussis is the Gram-negative bacterium responsible for whooping-cough. To develop a better understanding of the interactions between B. pertussis and the cells of colonized tissues, as well as for purposes of vaccine design, much effort has been devoted to characterizing the antigenic molecules exposed at its outer surface. A set of six U.S. Reference Factor Antisera raised by Eldering recognize agglutinogens that are specific to B. pertussis. Since each strain exhibits a particular subset of these antigens, the antisera provide a convenient means of classifying different strains of this bacterium. Several of these antigens have been identified at the molecular level. Serotype 1 agglutinogen is associated with a lipo-oligosaccharide, and those of serotypes 2 and 6 are fimbriae of two morphologically similar but antigenically distinct kinds. Recently, it has been demonstrated serotype 3 antiserum recognizes a 69kDa protein. To characterize the distribution of this antigen in and around B. pertussis cells, we have performed immuno-gold electron microscopy, using monoclonal antibodies raised against the 69kDa protein.

scholarly journals CULTIVATION OF BORDETELLA PERTUSSIS BIOFILMS ON ABIOTIC SUBSTRATE

2019 ◽  
Vol 1 (1) ◽  
pp. 49-53 ◽  
Author(s):  
E. M. Zaytsev ◽  
M. V. Britsina ◽  
M. N. Ozeretskovskaya ◽  
N. U. Mertsalova ◽  
I. G. Bazhanova
Keyword(s):  
Bordetella Pertussis ◽  
Nutrient Medium ◽  
Gentian Violet ◽  
Comparative Assessment ◽  
Original Strain ◽  
High Ability ◽  
Optimal Conditions ◽  
High Virulence ◽  
Different Strains ◽  
Selection Of

Aim. Selection of optimal conditions for the cultivation of Bordetella pertussis biofilms and comparative assessment of the ability of different strains to form biofilms. Materials and methods. Used a vaccine strain of B. pertussis № 475 and selected from this strain strain № 475а, characterized by high virulence. Cultures of strains grown on dense nutrient medium (DNM) and liquid nutrient medium (LNM) were used as inoculates for biofilms production. The intensity of biofilms formation in round-bottom 96-well polystyrene plates was estimated by staining with 0,1% gentian-violet solution. Results. Daily cultures of the strain № 475 with LNM formed moderate biofilms in the range of doses 5 — 1,25 IOU (international optical units) /ml, in the absence of growth at lower doses. The daily cultures of this strain with the DNM formed a dense biofilms when planting a doses in the range from 10 to 1,25 IOU/ml, moderate from 0,625 to 0,157 IOU/ml and weak biofilms at a dose of 0,079 IOU/ml. Strain № 475а with the DNM formed dense biofilms in the doses range of 10 to 0,04 IOU/ml and only at the dose of 0,02 IOU/ml were formed moderate biofilms. Conclusion. A simple and informative method has been developed to evaluate the ability of B. pertussis strains to form biofilms in polystyrene plates. Cultures obtained with the DNM formed a more significant biofilms than cultures with LNM. Identified high ability to biofilms formation by a selected strain №. 475a, compared to the original strain № 475.

scholarly journals Antibody Response from Whole-Cell Pertussis Vaccine Immunized Brazilian Children against Different Strains of Bordetella pertussis

2010 ◽  
Vol 82 (4) ◽  
pp. 678-682 ◽  
Author(s):  
Alexandre Pereira ◽  
Osvaldo A. Sant'Anna ◽  
Célio Lopes Silva ◽  
Aparecida S. Pietro Pereira ◽  
Ivo Lebrun ◽  
...  
Keyword(s):  
Antibody Response ◽  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Whole Cell ◽  
Different Strains ◽  
Brazilian Children

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

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