A replica plating method for CHO cells using nylon cloth

1975 ◽  
Vol 15 (6) ◽  
pp. 372-379 ◽  
Author(s):  
T.D. Stamato ◽  
Karig Hohmann
1965 ◽  
Vol 11 (4) ◽  
pp. 629-636 ◽  
Author(s):  
G. Stotzky

A replica plating method was developed to study ecology of microorganisms in soil. Precise placement of inocula and amendments at desired loci in sterile soil contained in petri plates were accomplished with a template. Subsequent growth and distribution of individual species, even when part of a mixed population, was measured by periodic transfer with an easily constructed replicator to agar plates of differing nutritional composition or containing selective inhibitors. The method is rapid and reproducible, and permits the study of many variables and interactions in a single soil plate; it can also be used with non-sterile soil and other suitable microbial habitats.


1977 ◽  
Vol 3 (4) ◽  
pp. 431-440 ◽  
Author(s):  
T. D. Stamato ◽  
Charles A. Waldren
Keyword(s):  

1977 ◽  
Vol 39 (2) ◽  
pp. 139-142 ◽  
Author(s):  
U. SCHULTE ◽  
M. H. ZENK

1967 ◽  
Vol 30 (6) ◽  
pp. 171-178 ◽  
Author(s):  
C. Vanderzant ◽  
G. D. Patel

Summary A replica plating method is described for a quantitative analysis of the microbial flora of certain milk and meat products. Species of Pseudomonas, Achromobacter, Alcaligenes, Flavobacterium or Cytophaga were present at the beginning of refrigerated storage. After holding for 10–20 days, species of Pseudomonas type I and II predominated in the milk samples. The microbial flora of the stored meats included species of greater number of genera.


1975 ◽  
Vol 21 (11) ◽  
pp. 1676-1680 ◽  
Author(s):  
David J. Bibel ◽  
Roko J. Smiljanic ◽  
Joseph R. LeBrun

A procedure of replica plating is described whereby all isolated colonies of Micrococcaceae can be identified with relative ease and rapidity. The method is as accurate as the recommended procedure, but permits a more complete and economical analysis of cutaneous flora in large-scale surveys. In this system, Baird-Parker carbohydrate medium was found somewhat superior to standard medium as was incubation at 35 °C instead of the customary 30 °C. Baird-Parker's broth medium for acetoin production yielded more positive results than did commercial medium, although the reactions were less distinct. However, an agar acetoin test medium was found as good or perhaps even better than Baird-Parker's medium. The classification schemes of Baird-Parker and Bergey's Manual were contrasted in the analysis of data.


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