A replica-plating method for the identification of Micrococcaceae

1975 ◽  
Vol 21 (11) ◽  
pp. 1676-1680 ◽  
Author(s):  
David J. Bibel ◽  
Roko J. Smiljanic ◽  
Joseph R. LeBrun

A procedure of replica plating is described whereby all isolated colonies of Micrococcaceae can be identified with relative ease and rapidity. The method is as accurate as the recommended procedure, but permits a more complete and economical analysis of cutaneous flora in large-scale surveys. In this system, Baird-Parker carbohydrate medium was found somewhat superior to standard medium as was incubation at 35 °C instead of the customary 30 °C. Baird-Parker's broth medium for acetoin production yielded more positive results than did commercial medium, although the reactions were less distinct. However, an agar acetoin test medium was found as good or perhaps even better than Baird-Parker's medium. The classification schemes of Baird-Parker and Bergey's Manual were contrasted in the analysis of data.

2018 ◽  
Vol 16 (1) ◽  
pp. 67-76
Author(s):  
Disyacitta Neolia Firdana ◽  
Trimurtini Trimurtini

This research aimed to determine the properness and effectiveness of the big book media on learning equivalent fractions of fourth grade students. The method of research is Research and Development  (R&D). This study was conducted in fourth grade of SDN Karanganyar 02 Kota Semarang. Data sources from media validation, material validation, learning outcomes, and teacher and students responses on developed media. Pre-experimental research design with one group pretest-posttest design. Big book developed consist of equivalent fractions material, students learning activities sheets with rectangle and circle shape pictures, and questions about equivalent fractions. Big book was developed based on students and teacher needs. This big book fulfill the media validity of 3,75 with very good criteria and scored 3 by material experts with good criteria. In large-scale trial, the result of students posttest have learning outcomes completness 82,14%. The result of N-gain calculation with result 0,55 indicates the criterion “medium”. The t-test result 9,6320 > 2,0484 which means the average of posttest outcomes is better than the average of pretest outcomes. Based on that data, this study has produced big book media which proper and effective as a media of learning equivalent fractions of fourth grade elementary school.


2021 ◽  
Vol 9 (3) ◽  
pp. 264
Author(s):  
Shanti Bhushan ◽  
Oumnia El Fajri ◽  
Graham Hubbard ◽  
Bradley Chambers ◽  
Christopher Kees

This study evaluates the capability of Navier–Stokes solvers in predicting forward and backward plunging breaking, including assessment of the effect of grid resolution, turbulence model, and VoF, CLSVoF interface models on predictions. For this purpose, 2D simulations are performed for four test cases: dam break, solitary wave run up on a slope, flow over a submerged bump, and solitary wave over a submerged rectangular obstacle. Plunging wave breaking involves high wave crest, plunger formation, and splash up, followed by second plunger, and chaotic water motions. Coarser grids reasonably predict the wave breaking features, but finer grids are required for accurate prediction of the splash up events. However, instabilities are triggered at the air–water interface (primarily for the air flow) on very fine grids, which induces surface peel-off or kinks and roll-up of the plunger tips. Reynolds averaged Navier–Stokes (RANS) turbulence models result in high eddy-viscosity in the air–water region which decays the fluid momentum and adversely affects the predictions. Both VoF and CLSVoF methods predict the large-scale plunging breaking characteristics well; however, they vary in the prediction of the finer details. The CLSVoF solver predicts the splash-up event and secondary plunger better than the VoF solver; however, the latter predicts the plunger shape better than the former for the solitary wave run-up on a slope case.


1965 ◽  
Vol 11 (4) ◽  
pp. 629-636 ◽  
Author(s):  
G. Stotzky

A replica plating method was developed to study ecology of microorganisms in soil. Precise placement of inocula and amendments at desired loci in sterile soil contained in petri plates were accomplished with a template. Subsequent growth and distribution of individual species, even when part of a mixed population, was measured by periodic transfer with an easily constructed replicator to agar plates of differing nutritional composition or containing selective inhibitors. The method is rapid and reproducible, and permits the study of many variables and interactions in a single soil plate; it can also be used with non-sterile soil and other suitable microbial habitats.


2012 ◽  
Vol 8 (S291) ◽  
pp. 375-377 ◽  
Author(s):  
Gregory Desvignes ◽  
Ismaël Cognard ◽  
David Champion ◽  
Patrick Lazarus ◽  
Patrice Lespagnol ◽  
...  

AbstractWe present an ongoing survey with the Nançay Radio Telescope at L-band. The targeted area is 74° ≲ l < 150° and 3.5° < |b| < 5°. This survey is characterized by a long integration time (18 min), large bandwidth (512 MHz) and high time and frequency resolution (64 μs and 0.5 MHz) giving a nominal sensitivity limit of 0.055 mJy for long period pulsars. This is about 2 times better than the mid-latitude HTRU survey, and is designed to be complementary with current large scale surveys. This survey will be more sensitive to transients (RRATs, intermittent pulsars), distant and faint millisecond pulsars as well as scintillating sources (or any other kind of radio faint sources) than all previous short-integration surveys.


2018 ◽  
Vol 5 (3) ◽  
pp. 172265 ◽  
Author(s):  
Alexis R. Hernández ◽  
Carlos Gracia-Lázaro ◽  
Edgardo Brigatti ◽  
Yamir Moreno

We introduce a general framework for exploring the problem of selecting a committee of representatives with the aim of studying a networked voting rule based on a decentralized large-scale platform, which can assure a strong accountability of the elected. The results of our simulations suggest that this algorithm-based approach is able to obtain a high representativeness for relatively small committees, performing even better than a classical voting rule based on a closed list of candidates. We show that a general relation between committee size and representatives exists in the form of an inverse square root law and that the normalized committee size approximately scales with the inverse of the community size, allowing the scalability to very large populations. These findings are not strongly influenced by the different networks used to describe the individuals’ interactions, except for the presence of few individuals with very high connectivity which can have a marginal negative effect in the committee selection process.


Energies ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 7422
Author(s):  
Min-Kyu Son

Upscaling of photoelectrode for a practical photoelectrochemical (PEC) water splitting system is still challenging because the PEC performance of large-scale photoelectrode is significantly low, compared to the lab scale photoelectrode. In an effort to overcome this challenge, sputtered gold (Au) and copper (Cu) grid lines were introduced to improve the PEC performance of large-scale cuprous oxide (Cu2O) photocathode in this work. It was demonstrated that Cu grid lines are more effective than Au grid lines to improve the PEC performance of large-scale Cu2O photocathode because its intrinsic conductivity and quality of grid lines are better than ones containing Au grid lines. As a result, the PEC performance of a 25-cm2 scaled Cu2O photocathode with Cu grid lines was almost double than one without grid lines, resulting in an improved charge transport in the large area substrate by Cu grid lines. Finally, a 50-cm2 scaled Cu2O photocathode with Cu grid lines was tested in an outdoor condition under natural sun. This is the first outdoor PEC demonstration of large-scale Cu2O photocathode with Cu grid lines, which gives insight into the development of efficient upscaled PEC photoelectrode.


2016 ◽  
Author(s):  
Dominik Paprotny ◽  
Oswaldo Morales Nápoles

Abstract. Large-scale hydrological modelling of flood hazard requires adequate extreme discharge data. Models based on physics are applied alongside those utilizing only statistical analysis. The former requires enormous computation power, while the latter are most limited in accuracy and spatial coverage. In this paper we introduce an alternate, statistical approach based on Bayesian Networks (BN), a graphical model for dependent random variables. We use a non-parametric BN to describe the joint distribution of extreme discharges in European rivers and variables describing the geographical characteristics of their catchments. Data on annual maxima of daily discharges from more than 1800 river gauge stations were collected, together with information on terrain, land use and climate of catchments that drain to those locations. The (conditional) correlations between the variables are modelled through copulas, with the dependency structure defined in the network. The results show that using this method, mean annual maxima and return periods of discharges could be estimated with an accuracy similar to existing studies using physical models for Europe, and better than a comparable global statistical method. Performance of the model varies slightly between regions of Europe, but is consistent between different time periods, and is not affected by a split-sample validation. The BN was applied to a large domain covering all sizes of rivers in the continent, both for present and future climate, showing large variation in influence of climate change on river discharges, as well as large differences between emission scenarios. The method could be used to provide quick estimates of extreme discharges at any location for the purpose of obtaining input information for hydraulic modelling.


2018 ◽  
Vol 7 (3) ◽  
pp. 298-304
Author(s):  
Reefa Qudsiya ◽  
Lisdiana Lisdiana ◽  
Nugrahaningsih WH

Nervous system teaching materials available in schools do not contain scientific-related content as mandated by the 2013 curriculum. In addition, teaching materials also do not contain student character reinforcement content. The purpose of this study is to develop “Nervous Problem Based Module with a  Conservation Character” as a nervous system learning supplement. This type of research is Research and Development (R&D) using ten steps, namely identification of potential and problems, data collection, product design, product design validation, product design revisions, small scale trials, product revision I, large scale trials, product revision II, and final products. The research subjects were students of class XI of SMA N 1 Bae Kudus. Nervous Problem Based Module with a  Conservation Character  tested the feasibility, readability, and effectiveness. Feasibility tests obtained very valid results. Readability test results were very valid. Test effectiveness by applying  Nervous Problem Based Module with a  Conservation Character in learning, obtained effective results and can be used in learning. The insertion of the conservation character reinforcement in the module gives positive results on the character of students. It can be concluded that “Nervous Problem Based Module with a Conservation Character” is decent and effectively applied in the nervous system learning.


2006 ◽  
Vol 3 (4) ◽  
pp. 777-803
Author(s):  
W. Connolley ◽  
A. Keen ◽  
A. McLaren

Abstract. We present results of an implementation of the Elastic Viscous Plastic (EVP) sea ice dynamics scheme into the Hadley Centre coupled ocean-atmosphere climate model HadCM3. Although the large-scale simulation of sea ice in HadCM3 is quite good with this model, the lack of a full dynamical model leads to errors in the detailed representation of sea ice and limits our confidence in its future predictions. We find that introducing the EVP scheme results in a worse initial simulation of the sea ice. This paper documents various improvements made to improve the simulation, resulting in a sea ice simulation that is better than the original HadCM3 scheme overall. Importantly, it is more physically based and provides a more solid foundation for future improvement. We then consider the interannual variability of the sea ice in the new model and demonstrate improvements over the HadCM3 simulation.


2021 ◽  
Vol 331 ◽  
pp. 06006
Author(s):  
Yossyafra ◽  
Anyta Ramadhani ◽  
Vina Gusman ◽  
Monica Herimarni

The COVID-19 pandemic has changed the world in various sectors and human activities. Limiting human activity and mobility also has an impact on transportation and traffic. This study aims to calculate the capacity and performance of roads under normal pandemic conditions before PSBB (Large-Scale Social Restrictions) in April 2020 and New Normal in July 2002, as well as predict traffic conditions if the Tsunami disaster hits the city during both periods. Tsunami Evacuation roads in Padang City were selected for analysis. The Indonesian Road Capacity Manual 1997 on urban roads is used as a reference for analyzing road performance indicators. The results showed that; road performance during the PSBB period was better than the New Normal period. The effect of volume and side traffic disturbance factors in the New Normal period makes a significant decrease in performance. Through prediction simulations, if a Tsunami occurs in the two study periods, the analyzed roads can relatively serve evacuation movements. However, the capacity needs to be increased for normal conditions.


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