Isolation of a lactic dehydrogenase-A-deficient CHO-K1 mutant by nylon cloth replica plating

1977 ◽  
Vol 3 (6) ◽  
pp. 639-647 ◽  
Author(s):  
T. D. Stamato ◽  
Carol Jones
Keyword(s):  
Lactic Dehydrogenase ◽  
Replica Plating ◽  
Nylon Cloth

Isolation of UV-sensitive variants of CHO-K1 by nylon cloth replica plating

1977 ◽  
Vol 3 (4) ◽  
pp. 431-440 ◽  
Author(s):  
T. D. Stamato ◽  
Charles A. Waldren
Keyword(s):  
Replica Plating ◽  
Nylon Cloth

A replica plating method for CHO cells using nylon cloth

1975 ◽  
Vol 15 (6) ◽  
pp. 372-379 ◽  
Author(s):  
T.D. Stamato ◽  
Karig Hohmann
Keyword(s):  
Cho Cells ◽  
Replica Plating ◽  
Plating Method ◽  
Nylon Cloth

Irisin Ameliorates Hypoxia/Reoxygenation-Induced Inflammation and Apoptosis in PC12 Cells by Inhibiting TLR4/MYD88 Signaling Pathway

2019 ◽  
Vol 17 (3) ◽  
pp. 329-336
Author(s):  
Wang Jinli ◽  
Xu Fenfen ◽  
Zheng Yuan ◽  
Cheng Xu ◽  
Zhang Piaopiao ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Signaling Pathway ◽  
Pc12 Cells ◽  
Major Complication ◽  
Lactic Dehydrogenase ◽  
Dependent Manner ◽  
Cerebral Ischemic Stroke ◽  
Cerebral Ischemic ◽  
Myd88 Signaling ◽  
Hypoxia Reoxygenation

Cardiovascular disease including cerebral ischemic stroke is the major complication that increases the morbidity and mortality in patients with diabetes mellitus as much as four times. It has been well established that irisin, with its ability to regulate glucose and lipid homeostasis as well as anti-inflammatory and anti-apoptotic properties, has been widely examined for its therapeutic potentials in managing metabolic disorders. However, the mechanism of irisin in the regulation of cerebral ischemic stroke remains unclear. Using PC12 cells as a model, we have shown that hypoxia/reoxygenation inhibits cell viability and increases lactic dehydrogenase. Irisin, in a dose-dependent manner, reversed these changes. The increase in inflammatory mediators (IL-1β, IL-6, and TNF-α) by hypoxia/reoxygenation was reversed by irisin. Furthermore, the cell apoptosis promoted by hypoxia/reoxygenation was also inhibited by irisin. Irisin suppressed TLR4/MyD88 signaling pathway leading to amelioration of inflammation and apoptosis in PC12 cells. Thus, inhibition of TLR4/MyD88 signaling pathway via irisin could be an important mechanism in the regulation of hypoxia/reoxygenation-induced inflammation and apoptosis in PC12 cells.

A multifactorial analysis of prognostic factors in patients with liver metastases from colorectal carcinoma.

1983 ◽  
Vol 1 (11) ◽  
pp. 720-726 ◽  
Author(s):  
C J Lahr ◽  
S J Soong ◽  
G Cloud ◽  
J W Smith ◽  
M M Urist ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Colorectal Carcinoma ◽  
Survival Rates ◽  
Elevated Serum ◽  
Hepatic Metastases ◽  
Lactic Dehydrogenase ◽  
Serum Bilirubin Level ◽  
Elevated Alkaline Phosphatase ◽  
Multifactorial Analysis ◽  
Primary Colorectal Tumor

A multifactorial analysis was used to identify the dominant prognostic variables predicting survival rates of 175 patients with hepatic metastases from colorectal carcinoma. Seven of 22 parameters examined simultaneously were found to independently influence the median survival rate in these patients: (1) elevated alkaline phosphatase (p = 0.0004), (2) elevated serum bilirubin level (p = 0.0005), (3) location of hepatic metastases (unilateral or bilateral, p = 0.0022), (4) number of metastatic nodes involved (0, 1-5, greater than 5; p = 0.0148), (5) depressed serum albumin (p = 0.0217), (6) whether or not the primary colorectal tumor was resected (p = 0.0013), and (7) chemotherapy (given or withheld, p = 0.0439). The prothrombin time, serum lactic dehydrogenase, and the number of hepatic metastases also correlated with survival, but they did not independently predict survival rates after other more dominant factors were accounted for. A mathematical equation for predicting an individual patient's clinical course once they developed hepatic metastases was derived from this statistical analysis. In addition, a simple and clinically useful guide for predicting outcome was developed that integrated the two most important risk factors, alkaline phosphatase and bilirubin.

Immunofluorescent antibody response to lactic dehydrogenase virus in different strains of mice

1992 ◽  
Vol 107 (2) ◽  
pp. 179-183 ◽  
Author(s):  
T. Hayashi ◽  
I. Mori ◽  
Y. Noguchi ◽  
T. Itoh ◽  
M. Saitoh
Keyword(s):  
Antibody Response ◽  
Lactic Dehydrogenase ◽  
Immunofluorescent Antibody ◽  
Different Strains

scholarly journals Lactic Dehydrogenase

1963 ◽  
Vol 238 (10) ◽  
pp. 3249-3255 ◽  
Author(s):  
David B.S. Millar ◽  
George W. Schwert
Keyword(s):  
Lactic Dehydrogenase
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