Giemsa banding patterns in the chromosomes of twelve species of cats (Felidae)

1973 ◽  
Vol 12 (6) ◽  
pp. 377-397 ◽  
Author(s):  
Doris H. Wurster-Hill ◽  
C.W. Gray
Keyword(s):  
Giemsa Banding ◽  
Banding Patterns

Identical Giemsa banding patterns of two Macaca species: Macaca mulatta and M. fascicularis

1975 ◽  
Vol 14 (1) ◽  
pp. 26-33 ◽  
Author(s):  
G.F. de Vries ◽  
H.F. de France ◽  
J.A.M. Schevers
Keyword(s):  
Macaca Mulatta ◽  
Giemsa Banding ◽  
Banding Patterns

Comparison of chromosome BrdU-Hoechst-Giemsa banding patterns of the A1 and (AD)2 genomes of cotton

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 616-625 ◽  
Author(s):  
Olga V. Muravenko ◽  
Alexander R. Fedotov ◽  
Elizabeth O. Punina ◽  
Ludmila I. Fedorova ◽  
Valerii G. Grif ◽  
...  
Keyword(s):  
Giemsa Banding ◽  
Banding Patterns

G and/or C-bands in plant chromosomes?

1984 ◽  
Vol 71 (1) ◽  
pp. 111-120
Author(s):  
I. Schubert ◽  
R. Rieger ◽  
P. Dobel
Keyword(s):  
Constitutive Heterochromatin ◽  
Giemsa Staining ◽  
Giemsa Banding ◽  
Base Pairs ◽  
Banding Patterns ◽  
C Banding ◽  
Plant Chromosomes ◽  
Nucleolus Organizing Region ◽  
Similarities And Differences ◽  
Simple Sequence

Similarities and differences become evident from comparisons of centromeric and non-centromeric banding patterns in plant and animal chromosomes. Similar to C and G-banding in animals (at least most of the reptiles, birds and mammals), centromeric and nucleolus-organizing region bands as well as interstitially and/or terminally located non-centromeric bands may occur in plants, depending on the kind and strength of pretreatment procedures. The last group of bands may sometimes be subdivided into broad regularly occurring ‘marker’ bands and thinner bands of more variable appearance. Non-centromeric bands in plants often correspond to blocks of constitutive heterochromatin that are rich in simple sequence DNA and sometimes show polymorphism; they thus resemble C-bands. However, most of these bands contain late-replicating DNA. Also they are sometimes rich A X T base-pairs, closely adjacent to each other and positionally identical to Feulgen+ and Q+ bands, thus being comparable to mammalian G-bands. Although banding that is reverse to the non-centromeric bands after Giemsa staining is still uncertain in plants, reverse banding patterns can be obtained with Feulgen or with pairs of A X T versus G X C-specific fluorochromes. It is therefore concluded that not all of the plant Giemsa banding patterns correspond to C-banding of mammalian chromosomes. Before the degree of homology between different Giemsa banding patterns in plants and G and/or C-bands in mammals is finally elucidated, the use of the neutral term ‘Giemsa band’, specified by position (e.g. centromeric, proximal, interstitial, terminal), is suggested to avoid confusion.

Comparison of chromosome BrdU-Hoechst-Giemsa banding patterns of the A1 and (AD)2 genomes of cotton

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 616-625 ◽  
Author(s):  
Olga V Muravenko ◽  
Alexander R Fedotov ◽  
Elizabeth O Punina ◽  
Ludmila I Fedorova ◽  
Valerii G Grif ◽  
...  
Keyword(s):  
Image Analysis ◽  
Complete Classification ◽  
Small Chromosome ◽  
Tetraploid Cotton ◽  
Image Analysis System ◽  
Giemsa Banding ◽  
Genome Chromosome ◽  
Banding Patterns ◽  
S Period ◽  
Analysis System

The karyotypes of diploid cotton, Gossypium herbaceum L. var. africanum (Watt) Mauer, and tetraploid cotton, Gossypium barbadense L., were studied by BrdU-Hoechst-Giemsa banding, using a specially developed image-analysis system. The patterns obtained are represented by the slightly and intensively stained bands that correspond, respectively, to the early replicating DNA and the DNA replicating in the mid and late S period. The number of main Giemsa-positive bands varies from 2 to 9 per chromosome. The banding patterns of all homologous pairs are specific in both the A1 and (AD)2 genomes. This made possible the complete classification of the chromosomes. Based on the similarity of the BrdU-Hoechst-Giemsa banding patterns and the sizes of the chromosomes in the A1 and (AD)2 genomes, we divided the (AD)2 genome into Ab and Db subgenomes and classified their chromosomes according to the A1 genome chromosome classification. The BrdU-Hoechst-Giemsa banding pattern of the Db subgenome is basically similar to that of the A1 genome and Ab subgenome, but the differences between it and the banding patterns of the A1 genome and Ab subgenome are more significant than the differences between the latter two genomes. The similarity of the intragenomic banding patterns between nonhomologous chromosomes a and b, c and g, d and e, f and j, h and i, and l and m was revealed. Based on our results, we suggest that the ancestral cotton genome contained 7 homologous pairs of chromosomes. The results prove the feasibility of image-analysis techniques for identification and quantitative analysis of chromosomes, especially with regard to small-chromosome species.Key words: cotton, A1 and (AD)2 genomes, chromosome identification, BrdU-Hoechst-Giemsa banding, image analysis.

GIEMSA-BANDING PATTERN OF MUNTJAC CHROMOSOMES

1973 ◽  
Vol 15 (2) ◽  
pp. 375-377 ◽  
Author(s):  
T. Sharma ◽  
G. S. Garg
Keyword(s):  
Banding Pattern ◽  
Giemsa Banding ◽  
Fluorescent Staining ◽  
Banding Patterns ◽  
Indian Muntjac

The Giemsa-banding patterns of Indian muntjac chromosomes stained after denaturation and renaturation of DNA were similar to the fluorescent staining patterns reported by others using quinacrine mustard.

CHROMOSOME STRUCTURE IN CHILOCORUS (COLEOPTERA: COCCINELLIDAE). I. FLUORESCENT AND GIEMSA BANDING PATTERNS

1974 ◽  
Vol 16 (3) ◽  
pp. 651-661 ◽  
Author(s):  
T. J. Ennis
Keyword(s):  
Chromosome Structure ◽  
Pericentric Heterochromatin ◽  
Giemsa Banding ◽  
Banding Patterns ◽  
Per Se ◽  
The Relationship ◽  
Karyotype Stability

Chromosomes of six species of Chilocorus have been examined for their reaction to Quinacrine (Q), and to Giemsa (G) after a variety of 'Denaturation-Renaturation' schedules and digestion with trypsin. Four types of chromatin can be distinguished with these techniques: 1, moderately stained with both Q and G; 2, brightly stained with Q and darkly stained with G; 3, unstained with Q but darkly stained with G; 4, unstained with Q but moderately stained with G. The last three of these types are restricted in chromosomally monomorphic species to the pericentric heterochromatin, but are variably distributed in the heterochromatic arms of C. stigma. Euchromatin per se does not react differentially. The relationship between karyotype stability and uniformity of banding patterns is discussed.

DIFFERENTIAL GIEMSA STAINING IN PLANTS. V. TWO TYPES OF CONSTITUTIVE HETEROCHROMATIN IN SPECIES OF AVENA

1977 ◽  
Vol 19 (4) ◽  
pp. 739-743 ◽  
Author(s):  
Sheng-Tian Yen ◽  
W. Gary Filion
Keyword(s):  
Room Temperature ◽  
Chromosome Banding ◽  
Constitutive Heterochromatin ◽  
Diploid Species ◽  
The Other ◽  
Giemsa Staining ◽  
Barium Hydroxide ◽  
Giemsa Banding ◽  
Banding Patterns ◽  
Hydrolysis Temperature

Modified ASG (Acetic/Saline/Giemsa) and BSG (Barium hydroxide/Saline/Giemsa) chromosome banding techniques applied to several diploid species of oats produced two distinct types of C-banding patterns. One pattern consisted mainly of centromeric bands with occasional telomeric and/or intercalary bands while the other was comprised only of prominent telomeric and intercalary bands. These two banding patterns which probably reflect two distinct types of constitutive heterochromatin resulted from a change in the HCl hydrolysis temperature prior to the application of the ASG or BSG technique; hydrolysis at 60 °C yielded the centromeric bands and hydrolysis at room temperature produced telomeric and intercalary bands. Since all species examined reacted in a similar manner, precise Giemsa banding patterns should now be possible for all or most species of oats.

Chromosome morphology in Gilbert's potoroo, Potorous gilbertii (Marsupialia : Potoroidae)

2000 ◽  
Vol 48 (3) ◽  
pp. 281 ◽  
Author(s):  
E. A. Sinclair ◽  
A. R. Murch ◽  
M. Di Renzo ◽  
M. Palermo
Keyword(s):  
Phylogenetic Analysis ◽  
Sex Chromosomes ◽  
Karyotype Evolution ◽  
Chromosome Morphology ◽  
Evolutionary Relationships ◽  
Giemsa Banding ◽  
Banding Patterns ◽  
Multiple Gene ◽  
Male And Female ◽  
Evolution Of Karyotypes

Chromosome morphology was examined for male and female Gilbert’s potoroo, Potorous gilbertii, to infer taxonomic and evolutionary relationships among the extant taxa within the genus Potorous. P. gilbertii has the same number of chromosomes as P. tridactylus, 2n = 12,13. Giemsa-banding patterns were very similar in P. gilbertii and P. tridactylus; however, differences were noted between the sex chromosomes. Given that the relationships among extant Potorous are unresolved, we mapped karyotypes onto two alternative phylogenies to suggest methods of karyotype evolution within this group. Karyotypes and molecular-based information from the now ‘presumed extinct’ P. platyops or sequencing of multiple gene regions for phylogenetic analysis within the Potoroidae would provide valuable information for resolving the issue of rooting, and hence drawing conclusions on the evolution of karyotypes within this group.

On the relevance of non-histone proteins to the production of Giemsa banding patterns on chromosomes

1974 ◽  
Vol 21 (3) ◽  
pp. 227-236 ◽  
Author(s):  
W. Vogel ◽  
J. Faust ◽  
M. Schmid ◽  
J.-W. Siebers
Keyword(s):  
Giemsa Banding ◽  
Banding Patterns ◽  
Histone Proteins
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