GIEMSA-BANDING PATTERN OF MUNTJAC CHROMOSOMES

1973 ◽  
Vol 15 (2) ◽  
pp. 375-377 ◽  
Author(s):  
T. Sharma ◽  
G. S. Garg
Keyword(s):  
Banding Pattern ◽  
Giemsa Banding ◽  
Fluorescent Staining ◽  
Banding Patterns ◽  
Indian Muntjac

The Giemsa-banding patterns of Indian muntjac chromosomes stained after denaturation and renaturation of DNA were similar to the fluorescent staining patterns reported by others using quinacrine mustard.

THE GIEMSA BANDING PATTERN OF THE AUSTRALIAN SWAMP BUFFALO (BUBALUS BUBALIS): CHROMOSOME HOMOLOGY WITH OTHER BOVIDAE

1976 ◽  
Vol 18 (2) ◽  
pp. 303-310 ◽  
Author(s):  
G. L. Toll ◽  
C. R. E. Halnan
Keyword(s):  
Y Chromosome ◽  
X Chromosome ◽  
Banding Pattern ◽  
Bubalus Bubalis ◽  
The Other ◽  
Giemsa Banding ◽  
Apparent Absence ◽  
Banding Patterns ◽  
Chromosome Homology ◽  
Swamp Buffalo

A Giemsa banding method was used to obtain preparations from which a G-band idiogram for the chromosomes of the Australian Swamp Buffalo (Bubalus bubalis) was constructed. Comparison with the G-banding patterns for goat, sheep, and ox chromosomes showed a remarkably close similarity between individual pairs, banding pattern homologies for the buffalo metacentric autosomes being identifiable among the acrocentric autosomes of the other species. However, the goat and sheep lacked a comparable autosome to the buffalo 10, the buffalo lacked an autosome comparable to the ox 12, the acrocentric X chromosome of the buffalo banded most closely to the goat X and was least like the ox. The buffalo Y chromosome was unlike its counterpart in the other species. The results are in keeping with the previously expressed view of evolution within the Bovidae by a Robertsonian mechanism modified by the apparent absence of one pair of autosomes from the buffalo and of a different pair from sheep and goats.

Giemsa banding patterns in the chromosomes of twelve species of cats (Felidae)

1973 ◽  
Vol 12 (6) ◽  
pp. 377-397 ◽  
Author(s):  
Doris H. Wurster-Hill ◽  
C.W. Gray
Keyword(s):  
Giemsa Banding ◽  
Banding Patterns

scholarly journals Involement of an acrosinlike proteinase in the sulfhydryl-induced degradation of rabbit sperm nuclear protamine

1980 ◽  
Vol 85 (1) ◽  
pp. 116-121 ◽  
Author(s):  
BR Zirkin ◽  
TSK Chang ◽  
J Heaps
Keyword(s):  
Proteolytic Activity ◽  
Banding Pattern ◽  
Basic Protein ◽  
Polyacrylamide Gels ◽  
Banding Patterns ◽  
Pattern Characteristic ◽  
Sperm Nuclei ◽  
Triton X ◽  
Esterase Inhibitor

Previous studies demonstrated that proteolytic activity is associated with isolated rabbit sperm nuclei and is responsible for the degradation of nuclear protamine that occurs during thiol-induced in vitro decondensation of the nuclei (Zirkin and Chang, 1977; Chang and Zirkin, 1978). In this study, we present the results of experiments designed to characterize this proteolytic activity. Basic protein isolated from rabbit sperm nuclei incubated with 5 mM dithiothreitol (DTT) and 1 percent Triton X-100 for increasing periods of time exhibited progressively faster migrating bands on acid-urea polyacrylamide gels, reflection the progressive degradation of protamine. Ultimately, a specific and characteristic peptide banding pattern resulted. When sperm nuclei were treated with the esterase inhibitor nitrophenyl-p-guanidino benzoate (NPGB) to inhibit the nuclear-associated proteolytic activity and then incubated with one of several exogenous proteinases in addition to DTT and Triton X-100, characteristic peptide banding patterns were seen for each exogenous proteinase employed. For trypsin, chymotrypsin, pronase, and papain, the peptide banding patterns differed from one another and from the pattern characteristic of protamine degradation by the nuclear-associated proteinase. By contrast, when rabbit acrosin served as the exogenous proteinase, the peptide banding pattern seen was identical to the pattern characteristic of the nuclear-associated proteinase. These results demonstrate directly that the proteinase associated with rabbit sperm nuclei and involved in sperm nuclear decondensation in vitro is acrosinlike.

Identical Giemsa banding patterns of two Macaca species: Macaca mulatta and M. fascicularis

1975 ◽  
Vol 14 (1) ◽  
pp. 26-33 ◽  
Author(s):  
G.F. de Vries ◽  
H.F. de France ◽  
J.A.M. Schevers
Keyword(s):  
Macaca Mulatta ◽  
Giemsa Banding ◽  
Banding Patterns

Comparison of chromosome BrdU-Hoechst-Giemsa banding patterns of the A1 and (AD)2 genomes of cotton

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 616-625 ◽  
Author(s):  
Olga V. Muravenko ◽  
Alexander R. Fedotov ◽  
Elizabeth O. Punina ◽  
Ludmila I. Fedorova ◽  
Valerii G. Grif ◽  
...  
Keyword(s):  
Giemsa Banding ◽  
Banding Patterns

A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 215-219 ◽  
Author(s):  
A. Zacharopoulou ◽  
K. Bourtzis ◽  
Ph. Kerremans
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Banding Pattern ◽  
Ceratitis Capitata ◽  
Polytene Chromosomes ◽  
Biological Significance ◽  
Fruit Fly ◽  
Banding Patterns ◽  
Homologous Chromosomes ◽  
Different Tissues

The banding patterns of polytene chromosomes in different tissues of the Mediterranean fruit fly, Ceratitis capitata, vary to such an extent that homologous chromosomes cannot be recognised. However, analyses of autosomal breakpoints in several translocation strains allowed chromosomes from the two tissues to be aligned despite their difference in banding pattern. These results were discussed, considering the different hypotheses of the origin and biological significance of polytene chromosome bands.Key words: polytene chromosomes, salivary gland chromosomes, orbital bristle trichogen cell chromosomes, Ceratitis capitata.

scholarly journals Purification of the chromosomes of the Indian muntjac by flow sorting.

1976 ◽  
Vol 24 (1) ◽  
pp. 348-354 ◽  
Author(s):  
A V Carrano ◽  
J W Gray ◽  
D H Moore ◽  
J L Minkler ◽  
B H Mayall ◽  
...  
Keyword(s):  
Large Fraction ◽  
Centromere Region ◽  
Chromosomal Dna ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Indian Muntjac ◽  
Flow Sorting ◽  
Flow Microfluorometry ◽  
Mechanical Shearing ◽  
High Degree

Metaphase chromosomes were isolated from a male Indian muntjac cell line, were stained with ethidium bromide and were analyzed by flow microfluorometry to establish a deoxyribonucleic acid (DNA)-based karyotype. Five major peaks were evident on the chromosomal DNA distribution corresponding to the five chromosome types in this species. The amount of DNA in each chromosome was confirmed by cytophotometric measurements of intact metaphase spreads. The five chromosome types were separated by flow sorting at rates up to several hundred chromosomes per second. The sorted chromosomes were identified by morphology and by Giemsa banding patterns. The automsomes, Numbers 1, 2 and 3, and the X + 3 composite chromosome were separated with a high degree of purity (90%). The centromere region of the X + 3 chromosome was fragile to mechanical shearing, and during isolation a small proportion of these chromosomes broke into four segiments: the long arm, the short arm, the short arm plus centromere and the centromere region. A large fraction of the constitutive heterochromatin of this species is present in the centromere region of the X + 3 chromosome and in the Y chromosome; these two regions possess similar amounts of DNA and therefore sort together. Chromosome flow sorting is rapid, reproducible and precise; it allows the collection of microgram quantities of purified chromosomes.

scholarly journals Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

2018 ◽  
Vol 19 (5) ◽  
pp. 1683-1689 ◽  
Author(s):  
NUR RAHMAH HIDAYATI ◽  
SURANTO SURANTO ◽  
SAJIDAN SAJIDAN
Keyword(s):  
Banding Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Retardation Factor ◽  
Cucurbita Moschata ◽  
Esterase Isozyme ◽  
Banding Patterns ◽  
Different Altitudes

Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

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