GIEMSA STAINING OF HETEROCHROMATIC AREAS OF HUMAN CHROMOSOMES FOR IDENTIFICATION

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner
Keyword(s):  
Acetic Acid ◽  
Banding Pattern ◽  
Saline Solution ◽  
Simple Technique ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Dna Denaturation ◽  
Quinacrine Fluorescence ◽  
Inexpensive Procedure ◽  
Distinct Banding Pattern

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.

QUINACRINE FLUORESCENCE AND Q-BANDING PATTERNS OF HUMAN CHROMOSOMES.: I. Effects of Varying Factors

1975 ◽  
Vol 17 (1) ◽  
pp. 81-92 ◽  
Author(s):  
C. C. Lin ◽  
H. van de Sande ◽  
W. K. Smink ◽  
D. R. Newton
Keyword(s):  
Exposure Time ◽  
Uv Light ◽  
Human Chromosomes ◽  
Concave Mirror ◽  
Banding Patterns ◽  
Fluorescent Microscope ◽  
Sperm Dna ◽  
Quinacrine Fluorescence ◽  
Well Differentiated ◽  
Salmon Sperm Dna

Various factors involved in the production of "Q-bands" have been studied. It was found that a Zeiss standard WL fluorescent microscope required a shorter exposure time for photography as compared to a Zeiss photomicroscope. The minimal exposure time was obtained when the standard WL microscope was equipped with a UV light source containing a DC powered mercury burner and a concave mirror. Further, the pH and type of water used in the staining, washing and mounting of the slide were also important factors in producing clear and well differentiated "Q-bands". It also appears that the factors involved in the production of "Q-bands" effect the enhancement or quenching of fluorescence by poly d(A-T).poly d(A-T) and salmon sperm DNA or poly dG∙poly dC respectively. This preliminary report also suggests that DNA or polynucleotides with a specific base sequence may play an important role in Q-banding patterns on chromosomes.

scholarly journals Differential Staining of Human Chromosomes by Treatment with Urea

1971 ◽  
Vol 47 (9) ◽  
pp. 729-731 ◽  
Author(s):  
Yukimasa SHIRAISHI ◽  
Tosihide H. YOSIDA
Keyword(s):  
Differential Staining ◽  
Human Chromosomes

Structural basis of banding pattern of human chromosomes

1973 ◽  
Vol 12 (2) ◽  
pp. 81-86 ◽  
Author(s):  
J. Cervenka ◽  
Hattie L. Thorn ◽  
R.J. Gorlin
Keyword(s):  
Banding Pattern ◽  
Structural Basis ◽  
Human Chromosomes

Spatial relations of human chromosomes identified by quinacrine fluorescence at metaphase

1973 ◽  
Vol 18 (4) ◽  
pp. 297-306 ◽  
Author(s):  
D. Warburton ◽  
A. F. Naylor ◽  
F. E. Warburton
Keyword(s):  
Spatial Relations ◽  
Human Chromosomes ◽  
Quinacrine Fluorescence

ADDITIONAL OBSERVATIONS ON THE PREPARATION OF R BANDED HUMAN CHROMOSOMES WITH ACRIDINE ORANGE

1976 ◽  
Vol 18 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Ram S. Verma ◽  
Herbert A. Lubs
Keyword(s):  
Acetic Acid ◽  
Acridine Orange ◽  
Careful Attention ◽  
Human Chromosomes ◽  
Ethanol Acetic Acid ◽  
Technical Factors

A number of technical factors which affect acridine orange R banding (RFA banding) were studied. These variables included age of slide, timing of fixation, details of incubation, mounting and the use of sequential technics. Optimal RFA banding was obtained between 15 and 20 days but good or very good preparations were obtained between 7 days and 2 months. Improved results were obtained in slides that were 3–4 months old by refixing the slides in ethanol acetic acid. Intermittent movement of slides during incubation in buffer as well as the details of mounting and removal of cover slips were found to be important. The best sequential banding was obtained with the sequence of Q to R but good results were obtained with the sequence G to R using ASG banding. Satisfactory results with the sequence R to C were not obtained. With careful attention to these variables good RFA banding can be obtained over a period of several months.

INFLUENCE OF CYSTEAMINE ON DIFFERENTIAL STAINING OF BUdR-SUBSTITUTED HUMAN CHROMOSOMES

1979 ◽  
Vol 21 (1) ◽  
pp. 145-149 ◽  
Author(s):  
W. Scheid
Keyword(s):  
Visible Light ◽  
Differential Staining ◽  
Human Chromosomes ◽  
Strand Breaks ◽  
Light System

In 5-bromodeoxyuridine (BUdR)-substituted human chromosomes stained with 4′-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.

A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP

2008 ◽  
Vol 28 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jörn Bullerdiek ◽  
Jürgen Dittmer ◽  
Angelika Faehre ◽  
Sabine Bartnitzke ◽  
Volker Kasche ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Human Chromosomes ◽  
Nick Translation ◽  
In Situ Nick Translation ◽  
Eco Ri

scholarly journals Management of Anterior Chamber Dislocation of a Fluocinolone Acetonide Implant: A Case Report

2020 ◽  
pp. 1-4
Author(s):  
Clara Monferrer Adsuara ◽  
Clara Monferrer Adsuara ◽  
Lucía Mata-Moret ◽  
Verónica Castro-Navarro ◽  
Javier Montero-Hernández
Keyword(s):  
Anterior Chamber ◽  
Saline Solution ◽  
Simple Technique ◽  
Fluocinolone Acetonide ◽  
Vitreous Cavity ◽  
Topical Anaesthesia ◽  
Case Presentation ◽  
Fluocinolone Acetonide Implant ◽  
Side Port ◽  
Uncommon Complication

Background: Fluocinolone acetonide implant (ILUVIEN) is a non-biodegradable cylindrical polyimide tube that is injected into the vitreous cavity. Migration to the anterior chamber can potentially occur, especially in patients with posterior capsular defects and vitrectomized eyes, although it is considered an uncommon complication. The best surgical technique is still unknown. We describe a simple technique for reinserting the migrated ILUVIEN implant in the posterior cavity without compromising its integrity. Case Presentation: Under topical anaesthesia, a corneal clear beveled limbal incision is made with a 20G angled side port blade. Balanced saline solution is injected with a 27G anterior chamber cannula to mobilize the implant and a reverse sinskey hook is then used to push the implant to the vitreous cavity between the iris and the intraocular lens without the need of viscoelastic. Conclusion: We report a simple and quick technique for surgical repositioning an ILUVIEN implant that required minimal manipulation and resulted in minimal tissue disturbance without compromising implant integrity and effectiveness. It is important to be cautious while using ILUVIEN in patients with capsular defects, zonular weakness, and previous vitrectomy. We recommend using a reverse sinskey hook as a smaller entry incision can be made to maintain the sealing of the anterior chamber.

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