Banding pattern observed in human chromosomes by the modified BSG technique

1976 ◽  
Vol 31 (3) ◽  
pp. 283-292 ◽  
Author(s):  
N. Kanda
1973 ◽  
Vol 12 (2) ◽  
pp. 81-86 ◽  
Author(s):  
J. Cervenka ◽  
Hattie L. Thorn ◽  
R.J. Gorlin

2008 ◽  
Vol 28 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jörn Bullerdiek ◽  
Jürgen Dittmer ◽  
Angelika Faehre ◽  
Sabine Bartnitzke ◽  
Volker Kasche ◽  
...  

1972 ◽  
Vol 14 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Jeffery P. Frey ◽  
Richard L. Neu ◽  
Harold O. Powers ◽  
Lytt I. Gardner

A simple technique is described for differential staining of human chromosomes with Giemsa. The procedure involves DNA denaturation with a methanol-acetic acid fixative, and subsequent annealing using a saline solution. This technique has a number of advantages over quinacrine fluorescence, and gives a more distinct banding pattern. It is a rapid, inexpensive procedure and can be done with existing equipment and material in most cytogenetic laboratories.


1972 ◽  
Vol 21 (4) ◽  
pp. 319-326
Author(s):  
G.L. Castoldi ◽  
G.D. Grusovin ◽  
G.L. Scapoli ◽  
R. Spanedda

SummaryThe acridine orange staining of metaphases previously treated with hot salt solutions, exhibits a differential banding pattern of the chromosomes. According to the physicochemical interpretation of the stained structures, the green and red fluorescent segments of the chromosomes should be considered as constituted respectively by double-stranded DNA and single-stranded DNA. The banding pattern is relatively consistent in different metaphases, although some occasional variations of the bands may be referred to the interference of chromosomal acid proteins. In general, the sequence of the bands is compatible with the picture of the reverse banding.


1971 ◽  
Vol 233 (37) ◽  
pp. 93-94 ◽  
Author(s):  
W. SCHNEDL

1984 ◽  
Vol 26 (3) ◽  
pp. 354-359 ◽  
Author(s):  
T. S. Zhan ◽  
S. Pathak ◽  
J. C. Liang

Pretreatment of embryos of a nondiapause strain of the migratory grasshopper (Melanoplus sanguinipes) with actinomycin D, followed by posttreatment of chromosome preparations obtained from them with NaOH and Sörenson's buffer, induced longitudinal differentiation of each chromosome after Giemsa staining. We consider such crossbands to be G-bands because of the following: (i) they were present along the entire length of each chromosome; (ii) the centromeric region of each chromosome was unstained; (iii) each pair of chromosomes showed its own distinctive banding pattern; (iv) the banding pattern was consistent from cell to cell for the same pair of chromosomes; and (v) a similar procedure is known to induce G-bands in human chromosomes. C-bands induced by the Ba(OH)2 technique showed constitutive heterochromatin to be confined to the centromeric region of each chromosome.Key words: Melanoplus, G-bands.


2012 ◽  
Vol 2 (12) ◽  
pp. 154-156 ◽  
Author(s):  
Priya Chaku ◽  
◽  
Pooja Shah
Keyword(s):  

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