scholarly journals Reactions of Subcutaneous Connective Tissue to Mineral Trioxide Aggregate, Biodentine®, and a Newly Developed BioACTIVE Base/Liner

Scanning ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Barış Karabulut ◽  
Nazmiye Dönmez ◽  
Ceren Canbey Göret ◽  
Cafer Ataş ◽  
Özlem Kuzu
Keyword(s):  
Connective Tissue ◽  
Mineral Trioxide Aggregate ◽  
Fibrous Capsule ◽  
Dystrophic Calcification ◽  
Bioactive Materials ◽  
Liner Material ◽  
Pulp Capping ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions

Aim. There is an increasing interest in the application of BioACTIVE materials to achieve hard tissue formation and maintain pulp vitality. Mineral trioxide aggregate (MTA) and Biodentine® are BioACTIVE materials used for pulp capping. Recently, dental researchers have produced BioACTIVE glass-incorporated light-curable pulp capping material. The study is aimed at evaluating the subcutaneous connective tissue reactions to MTA, Biodentine®, ACTIVA BioACTIVE Base/Liner. These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Sprague Dawley rats. The presence of inflammation, predominant cell type, calcification, and thickness of fibrous connective tissue was recorded by histological examination 7, 30, and 60 days after the implantation procedure. Scores were defined as follows: 0 = none or few inflammatory cells, no reaction; 1 = <25 cells, mild reaction; 2 = 25 to 125 cells, moderate reaction; and 3 = ≥125 cells, severe reaction. Fibrous capsule thickness, necrosis, and formation of calcification were recorded. ANOVA and post hoc Dunnett’s tests were used for statistically analyses (p<0.05). Results. In terms of oedema, inflammation, fibrous capsule, and necrosis, no significant differences were found in any time period for any material. MTA and Biodentine® showed higher calcification than in the ACTIVA BioACTIVE on day 30, and the difference was statistically significant (p<0.05). After 60 days, while calcification was not seen in the control group, it was observed in the test groups. There was a statistically significant difference between the control and the others. Conclusion. All materials were well tolerated by the tissues in the 60-day evaluation period. One notable finding is the presence of dystrophic calcification in the connective tissue adjacent to the newly developed BioACTIVE Base/Liner material. Therefore, this new BioACTIVE Base/Liner material may be safely recommended to clinicians as a pulp capping material.

Subcutaneous connective tissue reactions to iRoot SP, mineral trioxide aggregate (MTA) Fillapex, DiaRoot BioAggregate and MTA

2013 ◽  
Vol 47 (7) ◽  
pp. 667-674 ◽  
Author(s):  
C. C. Bósio ◽  
G. S. Felippe ◽  
E. A. Bortoluzzi ◽  
M. C. S. Felippe ◽  
W. T. Felippe ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Mineral Trioxide Aggregate ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions

Subcutaneous Connective Tissue Reactions to Three Types of Bioactive Glass Nanopowders

2011 ◽  
Vol 7 (3) ◽  
pp. 460-465 ◽  
Author(s):  
M. Mehdikhani-Nahrkhalaji ◽  
M. H. Fathi ◽  
V. Mortazavi ◽  
S. B. Mousavi ◽  
S. M. Razavi
Keyword(s):  
Connective Tissue ◽  
Bioactive Glass ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions

Antibacterial Effect of New Bioceramic Pulp Capping Material on the Main Cariogenic Bacteria

2016 ◽  
Vol 17 (5) ◽  
pp. 349-353 ◽  
Author(s):  
Fahiem MM Elshamy ◽  
Husham Elraih ◽  
Iti Gupta ◽  
Faisal AI Idris
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Mineral Trioxide Aggregate ◽  
Mutans Streptococci ◽  
Repair Material ◽  
Cariogenic Bacteria ◽  
Pulp Capping ◽  
Root Repair ◽  
Significant Difference

ABSTRACT Background The purpose of this research was to assess the antibacterial activity of a new bioceramic pulp capping material (endosequence root repair material [ERRM]) against the main cariogenic bacteria: Salivary mutans streptococci (MS) and lactobacilli and compare the results with mineral trioxide aggregate (MTA) and calcium hydroxide (Dycal). Materials and methods The isolation of MS group bacteria and Lactobacillus (LB) spp. from stimulated saliva was performed with in-office caries risk test bacteria dip slide test. Endosequence root repair material, MTA (ProRoot MTA), and Dycal were used as pulp capping materials. Mutans Streptococci and LB were scattered on the agar dishes with a swab. The pulp capping materials under study were placed in the wells and prepared in the agar, immediately after mixing. The dishes were incubated for 24 hours at 37°C. The growth inhibition zones were recorded and compared for every material and bacterial strain. One-way analysis of variance test was done to compare the development of growth inhibition of selected bacteria against testing materials. Post hoc Tukey honest significant difference was conducted to compare each material group. Results All the three selected pulp capping materials were found to inhibit the bacteria LB and MS. The antibacterial activity of ERRM and ProRoot MTA was significantly better than the Dycal. Against MS, ERRM and MTA showed no statistically significant difference. Mineral trioxide aggregate showed significantly better inhibitory activity against LB. Conclusion Endosequence root repair material and MTA had superior antibacterial properties against the main cariogenic bacteria: MS and LB compared with Dycal. Clinical significance A pulp-capping agent having good antibacterial properties can have better success rate in maintaining the vitality of the tooth while treating deep carious lesions in patients. How to cite this article Elshamy FMM, Singh G, Elraih H, Gupta I, AI Idris F. Antibacterial Effect of New Bioceramic Pulp Capping Material on the Main Cariogenic Bacteria. J Contemp Dent Pract 2016;17(5):349-353.

scholarly journals EFFECTS OF ODONTOCEM AND MINERAL TRIOXIDE AGGREGATE-ANGELUS TOXICITY ON FIBROBLAST VIABILITY

2019 ◽  
pp. 32-34
Author(s):  
DEWI SRI KESUMAWATI ◽  
ANGGRAINI MARGONO ◽  
ENDANG SUPRASTIWI
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
Pulp Capping ◽  
Diphenyltetrazolium Bromide ◽  
Significant Difference ◽  
Low Toxicity ◽  
Dental Applications ◽  
Chicken Eggs ◽  
Embryonated Chicken

Objective: Mineral trioxide aggregate (MTA) is proven to be biocompatible and is used for pulp capping treatment. Odontocem, calcium silicate-basedcement similar to MTA with the addition of a steroid, has recently been developed and to compare the effects of Odontocem and MTA-Angelus toxicityon fibroblast cell viability.Methods: Fibroblast cells from embryonated chicken eggs were immersed in Odontocem or MTA-Angelus solution for 24 and 72 h. Cell viability wasanalyzed by Microculture Tetrazolium (MTT) 3-(4,5-dimethylthiazol-2- yl)2,5-diphenyltetrazolium bromide assay.Results: After 24 h, the Odontocem and MTA-Angelus groups showed significantly lower viability (p<0.05) compared with controls. After 72 h, cellviability was significantly higher in the Odontocem and MTA-Angelus groups. However, there was no significant difference between the Odontocemand MTA-Angelus groups.Conclusions: Odontocem and MTA-Angelus have low toxicity for dental applications.

scholarly journals Tissue toxicity of root canal sealers

2004 ◽  
Vol 51 (3) ◽  
pp. 136-141 ◽  
Author(s):  
Mirjana Vujaskovic ◽  
Dragoljub Bacetic
Keyword(s):  
Connective Tissue ◽  
Root Canal ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Control Material ◽  
Tissue Sections ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions ◽  
Root Canal Sealers ◽  
Observation Period

The aim of this study was to evaluate tissue response to root canal sealers Tubuliseal and Sealapex. The sealers were freshly mixed and injected in the dorsal subcutaneous connective tissue of 12 Wistar rats.The observation periods were 7 days, 21days and 60 days. Four operative areas were formed ( 2 for test sealers, Tubliseal or Sealapex and 2 for control material) on each animal. Tissue sections were taken from selected sites. Each section included skin, subcutaneous connective tissue and underlying muscle tissue.All blocks were processed with the use of standardized histological procedures.The tissue reactions were studied under light microscopy. Different grades of tissue reaction to the tested materials were recorded as mild, moderate or severe inflammation. After seven days both root canal sealers showed severe inflammatory reaction of connective tissue in experimental animals.Tubuliseal caused prolonged moderate and mild inflammation. Sealapex caused mild inflammation which diminished at the end of the observation period. The results of this study demonstrated that Sealapex was better tolerated by tissue than Tubliseal.

scholarly journals Immediate response of human dental pulp capped with mineral trioxide aggregate, Portland cement and biodentin

2019 ◽  
Vol 45 (2) ◽  
pp. 108-116
Author(s):  
AKM Bashar ◽  
A.K.M Nurul Kabir ◽  
Rozina Akhter Rizdina ◽  
Ranjit Ghosh ◽  
Ashis Kumar Biswas ◽  
...  
Keyword(s):  
Portland Cement ◽  
Inflammatory Reaction ◽  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
Tissue Reaction ◽  
Control Group ◽  
Pulp Capping ◽  
Pulp Inflammation ◽  
Significant Difference ◽  
Human Dental Pulp

Background: The initial inflammatory reaction of pulp capping materials on the dental pulp has an intimate relation in promoting the future cellular differentiation and biomaterial mineralisation. So, analysis of immediate pulpal tissue reaction in vivo, is also important for evaluation of ultimate efficacy any pulp capping agent. To observe immediate inflammatory response of Human Dental Pulp capped with Mineral Trioxide Aggregate (MTA), Biodentin and Portland Cement (PC). Methods: A total of 70 permanent premolars teeth planned to be extracted for orthodontic alignment of occlusion were used as study sample. The teeth were divided into 3 experimental groups, MTA (n=20), Biodentin (n=20) Portland cement (n=20) and control group (n=10). After having an occlusal exposure of approximately 1.5 mm in diameter; in group A, pulp of teeth was capped with 2-mm-thick layer of ProRoot White MTA (Dentsply) and in group B, with sterile Biodentin (Septodont) according to the manufacturer’s recommendations. Whereas in group C, pulp of teeth was capped with sterile Portland Cement (PC). After placing the experimental material in each group, all teeth restored with glass i‹xiomer cement. After 24 hours the teeth were extracted, fixed in 10% buffered formalin solution, then decalcified by 10% nitric acid and embedded in paraffin. Finally, sectioned into 2 to 3-micron-thick serial sections in the linguo-buccal plane and stained with hematoxylin-eosin. After then the amount of pulp inflammation (type, intensity, and extension) were determined by using a predetermined evaluation criterion under an optical microscope at 40a magnification. Ten intact teeth, which received no exposure and pulp capping but extracted due to orthodontic purpose were also collected and treated as the control group (group D); undergone same histologic preparation and evaluation. Significantstatistical differences among the experimental groups were to be found (p<0.05). Results: Histologically, all the three tested materials produced immediate pulpal tissue reaction. ‘Biodentin’ found to be most immediate pulpal tissue reactive (reactive in 100% cases) and ‘Portland Cement’ showed least immediate tissue reaction (only in 30.0% cases). whereas, MTA produced immediate tissue reaction only in 50.0% cases. Immediate pulpal inflammatory reaction in response to tested material found to be statistically significant different between ‘Biodentin’ and ‘Portland cmient’ (p=0.01), also between ‘Biodentin’ and ‘MTA’ and (p=0.001); but there was no statistically significant difference between ‘MTA’ and ‘Portland cement’ (p =0.197). Conclusion: Considering the maximum immediate pulpal tissue reaction (Inflammation), Biodentin is expected to produce most favorable ultimate bioactivity (biomaterial mineralization) after pulp capping. Bangladesh Med Res Counc Bull 2019; 45: 108-116

scholarly journals Comparison of Rat Connective Tissue Response to BioMTA, Angelus MTA, and Root MTA

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Marzieh Alimohammadi ◽  
Sina Mirzaee-Rad ◽  
Farideh Feizi ◽  
Hadi Shirzad Juybari ◽  
Akam Saeidi ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Wistar Rats ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Control Group ◽  
Capsule Formation ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Difference ◽  
Fibrous Capsule Formation

Due to the widespread use of MTA in dentistry and various brands of this product, we decided to compare the three brands available in the country market by their biocompatibility. We divided 20 male Wistar rats into four groups. After local anesthesia and washing, we made two incisions on both sides (4 incisions in total). The experimental groups were Angelus MTA (Angelus, Brazil), BioMTA (CERKAMED, Poland), Root MTA (Dr. Lotfi, Tabriz, Iran), and the control group. The resulting paste was placed in a tube and implanted subcutaneously into male Wistar rats. Wistar rats were sacrificed 7, 15, 30, and 60 days later, with high anesthetic doses. The sample implanted in 10% formalin was stabilized after tissue processing and H&E staining under a microscope. The inflammatory reaction in the tissues received different scores at the beginning of the tube opening. BioMTA had the highest inflammatory response among the groups, but the difference was not statistically significant ( p  > 0.05). Also, there was no significant difference between the groups’ granulation and calcification ( p  < 0.05). There was a significant difference between BioMTA, Angelus MTA, Root MTA, and control groups in fibrous capsule formation ( p  < 0.05). Angelus MTA showed the lowest mean fibrous capsule formation in all periods. The effects of Angelus MTA, Root MTA, and BioMTA on connective tissue were investigated and compared. According to this study, these materials have good biocompatibility. According to the findings and statistical analysis, Angelus MTA has the most biocompatibility.

scholarly journals A Confocal Laser Scanning Microscopic Study Evaluating the Sealing Ability of Mineral Trioxide Aggregate, Biodentine and Anew Pulp Capping Agent-Theracal

2015 ◽  
Vol 03 (01) ◽  
pp. 020-025 ◽  
Author(s):  
Sameer Makkar ◽  
Harpreet Kaur ◽  
Anurag Aggarwal ◽  
Ruchi Vashisht
Keyword(s):  
Laser Scanning ◽  
Mineral Trioxide Aggregate ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Capping Agent ◽  
Capping Agents ◽  
Pulp Capping ◽  
Sealing Ability ◽  
Group I ◽  
Significant Difference

Abstract Aims and Objectives: Viability and health of the pulpal tissue after an exposure can be stimulated with biocompatible pulp capping materials. Mineral trioxide aggregate(MTA), though biocompatible with excellent sealing ability, has clinical disadvantages like poor handling properties and long setting time. New pulp capping agents- Biodentine and Theracal have proven to overcome MTA’s shortcomings. This study aimed to comparatively evaluate the sealing ability of Mineral Trioxide Aggregate(Angelus), Biodentine (Septodont) and Theracal(Bisco) when used as pulp capping agents. Materials and Methods: Occlusal cavities were prepared in extracted human third molars. The cavities were divided into 3 groups containing 12 samples each. Pulp capping in samples of group I, II and III was done with MTA, Biodentine and Theracal respectively. All samples were kept in a damp environment for 24 hrs. In each tooth, the root was cut just below the cementum-enamel junction. An aqueous 0.25% solution of Rhodamine-B was put in the open pulp chambers of all the samples and placed upside-down in a damp environment. The dye was left for 3 hrs to permeate toward the interface. After being stained, all samples were rinsed with distilled water and sectioned vertically. A Confocal Laser Scanning Microscope was used to image the samples. Results: No significant difference was found in interfacial microleakage between MTA and Biodentine. Theracal exhibited less interfacial microleakage than the two. Conclusion: Biodentine and MTA exhibit an efficient and durable protection of pulp. Theracal exhibits better sealing ability as a pulp capping agent than MTA and Biodentine.

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