Subcutaneous connective tissue reactions to iRoot SP, mineral trioxide aggregate (MTA) Fillapex, DiaRoot BioAggregate and MTA

2013 ◽  
Vol 47 (7) ◽  
pp. 667-674 ◽  
Author(s):  
C. C. Bósio ◽  
G. S. Felippe ◽  
E. A. Bortoluzzi ◽  
M. C. S. Felippe ◽  
W. T. Felippe ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Mineral Trioxide Aggregate ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions

scholarly journals Reactions of Subcutaneous Connective Tissue to Mineral Trioxide Aggregate, Biodentine®, and a Newly Developed BioACTIVE Base/Liner

Scanning ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Barış Karabulut ◽  
Nazmiye Dönmez ◽  
Ceren Canbey Göret ◽  
Cafer Ataş ◽  
Özlem Kuzu
Keyword(s):  
Connective Tissue ◽  
Mineral Trioxide Aggregate ◽  
Fibrous Capsule ◽  
Dystrophic Calcification ◽  
Bioactive Materials ◽  
Liner Material ◽  
Pulp Capping ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions

Aim. There is an increasing interest in the application of BioACTIVE materials to achieve hard tissue formation and maintain pulp vitality. Mineral trioxide aggregate (MTA) and Biodentine® are BioACTIVE materials used for pulp capping. Recently, dental researchers have produced BioACTIVE glass-incorporated light-curable pulp capping material. The study is aimed at evaluating the subcutaneous connective tissue reactions to MTA, Biodentine®, ACTIVA BioACTIVE Base/Liner. These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Sprague Dawley rats. The presence of inflammation, predominant cell type, calcification, and thickness of fibrous connective tissue was recorded by histological examination 7, 30, and 60 days after the implantation procedure. Scores were defined as follows: 0 = none or few inflammatory cells, no reaction; 1 = <25 cells, mild reaction; 2 = 25 to 125 cells, moderate reaction; and 3 = ≥125 cells, severe reaction. Fibrous capsule thickness, necrosis, and formation of calcification were recorded. ANOVA and post hoc Dunnett’s tests were used for statistically analyses (p<0.05). Results. In terms of oedema, inflammation, fibrous capsule, and necrosis, no significant differences were found in any time period for any material. MTA and Biodentine® showed higher calcification than in the ACTIVA BioACTIVE on day 30, and the difference was statistically significant (p<0.05). After 60 days, while calcification was not seen in the control group, it was observed in the test groups. There was a statistically significant difference between the control and the others. Conclusion. All materials were well tolerated by the tissues in the 60-day evaluation period. One notable finding is the presence of dystrophic calcification in the connective tissue adjacent to the newly developed BioACTIVE Base/Liner material. Therefore, this new BioACTIVE Base/Liner material may be safely recommended to clinicians as a pulp capping material.

Subcutaneous Connective Tissue Reactions to Three Types of Bioactive Glass Nanopowders

2011 ◽  
Vol 7 (3) ◽  
pp. 460-465 ◽  
Author(s):  
M. Mehdikhani-Nahrkhalaji ◽  
M. H. Fathi ◽  
V. Mortazavi ◽  
S. B. Mousavi ◽  
S. M. Razavi
Keyword(s):  
Connective Tissue ◽  
Bioactive Glass ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions

scholarly journals Tissue toxicity of root canal sealers

2004 ◽  
Vol 51 (3) ◽  
pp. 136-141 ◽  
Author(s):  
Mirjana Vujaskovic ◽  
Dragoljub Bacetic
Keyword(s):  
Connective Tissue ◽  
Root Canal ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Control Material ◽  
Tissue Sections ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions ◽  
Root Canal Sealers ◽  
Observation Period

The aim of this study was to evaluate tissue response to root canal sealers Tubuliseal and Sealapex. The sealers were freshly mixed and injected in the dorsal subcutaneous connective tissue of 12 Wistar rats.The observation periods were 7 days, 21days and 60 days. Four operative areas were formed ( 2 for test sealers, Tubliseal or Sealapex and 2 for control material) on each animal. Tissue sections were taken from selected sites. Each section included skin, subcutaneous connective tissue and underlying muscle tissue.All blocks were processed with the use of standardized histological procedures.The tissue reactions were studied under light microscopy. Different grades of tissue reaction to the tested materials were recorded as mild, moderate or severe inflammation. After seven days both root canal sealers showed severe inflammatory reaction of connective tissue in experimental animals.Tubuliseal caused prolonged moderate and mild inflammation. Sealapex caused mild inflammation which diminished at the end of the observation period. The results of this study demonstrated that Sealapex was better tolerated by tissue than Tubliseal.

Reaction of Rat Subcutaneous Connective Tissue to a Mineral Trioxide Aggregate–based and a Zinc Oxide and Eugenol Sealer

2012 ◽  
Vol 38 (9) ◽  
pp. 1233-1238 ◽  
Author(s):  
Osvaldo Zmener ◽  
Ricardo Martinez Lalis ◽  
Cornelis H. Pameijer ◽  
Carolina Chaves ◽  
Gabriel Kokubu ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Connective Tissue ◽  
Mineral Trioxide Aggregate ◽  
Subcutaneous Connective Tissue

scholarly journals Comparison of the biocompatibility of grey mineral trioxide aggregate and sealapex plus zinc oxide in rat subcutaneous tissue

2015 ◽  
Vol 21 (3) ◽  
pp. 136
Author(s):  
Waldécio Vita ◽  
Mitermayer Galvão Reis ◽  
Theo Araujo Santos ◽  
Ana Maria Carvalho ◽  
Cristina Mota ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Connective Tissue ◽  
Granulation Tissue ◽  
Subcutaneous Tissue ◽  
Dental Materials ◽  
Mineral Trioxide Aggregate ◽  
Tissue Response ◽  
Tissue Samples ◽  
Biocompatibility Testing ◽  
Tissue Reactions

ABSTRACT   Objectives:The aim of this study was to compare the subcutaneous tissue response to grey mineral trioxide aggregate white Sealapex plus zinc oxide.   Methods: Polyethylene tubes filled with tested material were implanted in the connective tissue of rats. Control animals received empty tubes. Tissue samples were collected after 7, 60, and 90 days and stained with hematoxylin-eosin, picrosirius-fast green, and von Kossa stain for morphological analysis. The connective tissue response to the implanted materials was evaluated descriptively and semi-quantitatively by scoring the degree of inflammation, granulation tissue formation, fibrosis, and calcification. Results: Examinations of the grey mineral trioxide aggregate group over time revealed more intense inflammation at 7 days than at 60 days (p <0.05). In the Sealapex plus zinc oxide group, granulation tissue was more abundant at 7 days than at 60 days (p <0.05). Regarding calcification, von Kossa-positive granules were observed in the grey mineral trioxide aggregate and Sealapex plus zinc oxide  groups at all time points studied. In the Sealapex/ZnO group, calcification was more apparent at 60 days than at 7 days (p <0.05). Relevance: This study demonstrates that all tested materials promote similar tissue reactions. Descriptors: Biocompatibility Testing, Endodontics, Dental Materials, Retrograde Obturation. 

scholarly journals Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

2008 ◽  
Vol 19 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Ana Teresa Sant'anna ◽  
Luis Carlos Spolidório ◽  
Lizeti Toledo Oliveira Ramalho
Keyword(s):  
Connective Tissue ◽  
Chronic Inflammation ◽  
Histological Analysis ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Connective Tissues ◽  
Plastic Tube ◽  
Tissue Samples ◽  
Subcutaneous Connective Tissue ◽  
Original Formula

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

Sign in / Sign up

Export Citation Format

Share Document