scholarly journals Synthesis and Characterization of a New Collagen-Alginate Aerogel for Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Abraham Muñoz-Ruíz ◽  
Diana M. Escobar-García ◽  
Mildred Quintana ◽  
Amaury Pozos-Guillén ◽  
Héctor Flores
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Supercritical Drying ◽  
Drying Process ◽  
Sem Images ◽  
Promote Cell Migration ◽  
Carbon Based Nanomaterials ◽  
Highly Porous ◽  
Alginate Scaffold

Scaffolds have been used as extracellular matrix analogs to promote cell migration, cell attachment, and cell proliferation. The use of aerogels and carbon-based nanomaterials has recently been proposed for tissue engineering due to their properties. The aim of this study is to develop a highly porous collagen-alginate(-graphene oxide) aerogel-based scaffold. The GO synthesis was performed by Hummers method; a collagen-alginate and collagen-alginate-GO hydrogel were synthetized; then, they were treated by a supercritical drying process. The aerogels obtained were evaluated by SEM and FTIR. Osteoblasts were seeded over the scaffolds and evaluated by SEM. According to the characterization, the aerogels showed a highly porous interconnected network covered by a nonporous external wall. According to the FTIR, the chemical functional groups of collagen and GO were maintained after the supercritical process. The SEM images after cell culture showed that a collagen-alginate scaffold promotes cell attachment and proliferation. The alginate-collagen aerogel-based scaffold could be a platform for tissue engineering since it shows adequate properties. Further studies are needed to determine the cell interactions with GO.

Characterization of novel calcium hydroxide‐mediated highly porous chitosan‐calcium scaffolds for potential application in dentin tissue engineering

2020 ◽  
Vol 108 (6) ◽  
pp. 2546-2559 ◽  
Author(s):  
Diana Gabriela Soares ◽  
Ester Alves Ferreira Bordini ◽  
Fernanda Balestrero Cassiano ◽  
Erika Soares Bronze‐Uhle ◽  
Leandro Edgar Pacheco ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Hydroxide ◽  
Potential Application ◽  
Porous Chitosan ◽  
Highly Porous

scholarly journals Fabrication and Biological Analysis of Highly Porous PEEK Bionanocomposites Incorporated with Carbon and Hydroxyapatite Nanoparticles for Biological Applications

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3572
Author(s):  
P. D. Swaminathan ◽  
Md. Nizam Uddin ◽  
P. Wooley ◽  
Ramazan Asmatulu
Keyword(s):  
Bone Marrow ◽  
Cell Viability ◽  
Bone Marrow Cells ◽  
Cell Attachment ◽  
Marrow Cell ◽  
Biological Properties ◽  
Carbon Particles ◽  
Biological Tests ◽  
Carbon Based Nanomaterials ◽  
Highly Porous

Bone regeneration for replacing and repairing damaged and defective bones in the human body has attracted much attention over the last decade. In this research, highly porous polyetheretherketone (PEEK)/hydroxyapatite (HA) bionanocomposite scaffolds reinforced with carbon fiber (CF) and carbon nanotubes (CNTs) were fabricated, and their structural, mechanical, and biological properties were studied in detail. Salt porogen (200–500 µm size) leaching methods were adapted to produce porous PEEK structures with controlled pore size and distribution, facilitating greater cellular infiltration and biological integration of PEEK composites within patient tissue. In biological tests, nanocomposites proved to be non-toxic and have very good cell viability. In addition, bone marrow cell growth was observed, and PEEK/HA biocomposites with carbon particles showed increased cell attachment over the neat PEEK/HA composites. In cell viability tests, bionanocomposites with 0.5 wt% CNTs established good attachment of cells on disks compared to neat PEEK/HA biocomposites. A similar performance was seen in culture tests of bone marrow cells (osteoblasts and osteoclasts). The 0.5 wt% CF for osteoblasts and 1 wt% CNTs for osteoclasts showed higher cell attachment. The addition of carbon-based nanomaterials into PEEK/HA has been identified as an effective approach to improve cell attachment as well as mechanical and biological properties. With confirmed cell attachment and sustained viability and proliferation of the fabricated PEEK/HA/CNTs, CF bionanocomposites were confirmed to possess excellent biocompatibility and will have potential uses in bone scaffolding and other biomedical applications.

Effect of the drying on morphology and texture of aerogels and zirconia cryogels

MRS Advances ◽  
2019 ◽  
Vol 4 (64) ◽  
pp. 3513-3521
Author(s):  
Tzipatly A. Esquivel-Castro ◽  
Antonia Martínez-Luévanos ◽  
Luis Alfonso García-Cerda ◽  
Juan C. Contreras-Esquivel ◽  
Pascual Bartolo Pérez ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cetyltrimethylammonium Bromide ◽  
Sol Gel ◽  
Three Dimensional ◽  
Supercritical Drying ◽  
Sem Images ◽  
Texture Properties ◽  
Dimensional Network ◽  
Zirconia Aerogel

ABSTRACTDue to their excellent properties, aerogel has attracted the attention of the scientific community to use it in the biomedical area as a drug delivery system. This work reports on the synthesis and characterization of ZrO2 aerogels and cryogels obtained by the sol-gel method. The influence of different cetyltrimethylammonium bromide (CTAB) and the type of drying on structural, morphological and texture properties of ZrO2 aerogels and cryogels was investigated. SEM images reveal that a porous interconnected three-dimensional network was formed into aerogels due to supercritical drying. Zirconia aerogel sample has a specific surface area (SBET) larger than zirconia cryogels. Therefore, our results indicate that zirconia aerogel is an adequate material for applications in drug delivery systems.

scholarly journals Porous Lactose-Modified Chitosan Scaffold for Liver Tissue Engineering: Influence of Galactose Moieties on Cell Attachment and Mechanical Stability

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Birong Wang ◽  
Qinggang Hu ◽  
Tao Wan ◽  
Fengxiao Yang ◽  
Le Cui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Sessile Drop ◽  
Mechanical Stability ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Sem Images ◽  
Hemolysis Assay ◽  
Modified Chitosan ◽  
Liver Tissue Engineering

Galactosylated chitosan (CTS) has been widely applied in liver tissue engineering as scaffold. However, the influence of degree of substitution (DS) of galactose moieties on cell attachment and mechanical stability is not clear. In this study, we synthesized the lactose-modified chitosan (Lact-CTS) with various DS of galactose moieties by Schiff base reaction and reducing action of NaBH4, characterized by FTIR. The DS of Lact-CTS-1, Lact-CTS-2, and Lact-CTS-3 was 19.66%, 48.62%, and 66.21% through the method of potentiometric titration. The cell attachment of hepatocytes on the CTS and Lact-CTS films was enhanced accompanied with the increase of galactose moieties on CTS chain because of the galactose ligand-receptor recognition; however, the mechanical stability of Lact-CTS-3 was reduced contributing to the extravagant hydrophilicity, which was proved using the sessile drop method. Then, the three-dimensional Lact-CTS scaffolds were fabricated by freezing-drying technique. The SEM images revealed the homogeneous pore bearing the favorable connectivity and the pore sizes of scaffolds with majority of 100 μm; however, the extract solution of Lact-CTS-3 scaffold significantly damaged red blood cells by hemolysis assay, indicating that exorbitant DS of Lact-CTS-3 decreased the mechanical stability and increased the toxicity. To sum up, the Lact-CTS-2 with 48.62% of galactose moieties could facilitate the cell attachment and possess great biocompatibility and mechanical stability, indicating that Lact-CTS-2 was a promising material for liver tissue engineering.

scholarly journals Characterization of biodegradable poly(lactic acid) porous scaffolds prepared using selective enzymatic degradation for tissue engineering

RSC Advances ◽  
2017 ◽  
Vol 7 (54) ◽  
pp. 34063-34070 ◽  
Author(s):  
Ziqi Guo ◽  
Cheng Yang ◽  
Zuping Zhou ◽  
Shan Chen ◽  
Fan Li
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Enzymatic Degradation ◽  
Poly Lactic Acid ◽  
Porous Scaffolds ◽  
Sem Images ◽  
Biodegradable Poly

SEM images of MEF cells on PLA scaffolds prepared by selective enzymatic degradation after 7 days of culture. The results demonstrated that MEF cells attached more easily to the surface than in the interior of the PLA scaffolds.

Synthesis and characterization of an enzyme-degradable zwitterionic dextran hydrogel

RSC Advances ◽  
2016 ◽  
Vol 6 (37) ◽  
pp. 30862-30866 ◽  
Author(s):  
Haiyan Wu ◽  
Huifeng Wang ◽  
Fang Cheng ◽  
Fujian Xu ◽  
Gang Cheng
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Matrix Metalloproteinase ◽  
Protein Adsorption ◽  
Cell Attachment ◽  
Synthesis And Characterization ◽  
Engineering Applications ◽  
Soft Tissue Engineering ◽  
Nonspecific Protein Adsorption

A matrix metalloproteinase peptide cross-linked dextran hydrogel was synthesized. Dextran was modified with carboxybetaine to resist nonspecific protein adsorption and cell attachment. The degradable hydrogel is a good candidate for soft tissue engineering applications.

Fabrication and Characterization of Hydrophilized Polydioxanone Scaffolds for Tissue Engineering Applications

2007 ◽  
Vol 342-343 ◽  
pp. 289-292 ◽  
Author(s):  
Kwang Joon Cho ◽  
Dae Keun Song ◽  
Se Heang Oh ◽  
Young Joo Koh ◽  
Sahng Hoon Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Engineering Applications ◽  
Tissue Compatibility ◽  
Cell Tissue ◽  
Particulate Leaching ◽  
Fabrication And Characterization ◽  
Highly Porous

Porous polydioxanone (PDO)/polyvinyl alcohol (PVA) scaffolds were fabricated by blending PDO with a small amount of PVA to improve the hydrophilicity and cell/tissue compatibility of the scaffolds for tissue engineering applications. PDO/PVA scaffolds with different PVA compositions up to 10 wt% were fabricated by a melt-molding particulate-leaching method (non-solvent method). The prepared scaffolds exhibited highly porous, uniform open-cellular pore structures. The PDO/PVA scaffolds with PVA compositions more than 5 % were easily wetted in cell culture medium. The hydrophilized PDO/PVA (5 wt%) scaffold showed better cell adhesion and growth than the control hydrophobic PDO scaffold. The PDO/PVA (5 wt%) scaffold also showed faster tissue infiltration into the scaffold than the PDO scaffold. It seems that 5 wt% addition of PVA to PDO to fabricate PDO/PVA scaffolds is enough for improving the hydrophilicity and cell/tissue compatibility of the scaffolds.

scholarly journals Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds

2016 ◽  
Vol 2016 ◽  
pp. 1-16 ◽  
Author(s):  
Nergis Abay ◽  
Gorke Gurel Pekozer ◽  
Mustafa Ramazanoglu ◽  
Gamze Torun Kose
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Attachment ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Polybutylene Succinate ◽  
Tooth Germs ◽  
Surface Modified ◽  
First Time

Designing and providing a scaffold are very important for the cells in tissue engineering. Polybutylene succinate (PBS) has high potential as a scaffold for bone regeneration due to its capacity in cell proliferation and differentiation. Also, stem cells from 3rd molar tooth germs were favoured in this study due to their developmentally and replicatively immature nature. In this study, porcine dental germ stem cells (pDGSCs) seeded PBS scaffolds were used to investigate the effects of surface modification with fibronectin or laminin on these scaffolds to improve cell attachment, proliferation, and osteogenic differentiation for tissue engineering applications. The osteogenic potentials of pDGSCs on these modified and unmodified foams were examined to heal bone defects and the effects of fibronectin or laminin modified PBS scaffolds on pDGSC differentiation into bone were compared for the first time. For this study, MTS assay was used to assess the cytotoxic effects of modified and unmodified surfaces. For the characterization of pDGSCs, flow cytometry analysis was carried out. Besides, alkaline phosphatase (ALP) assay, von Kossa staining, real-time PCR, CM-Dil, and immunostaining were applied to analyze osteogenic potentials of pDGSCs. The results of these studies demonstrated that pDGSCs were differentiated into osteogenic cells on fibronectin modified PBS foams better than those on unmodified and laminin modified PBS foams.

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